FastQCFastQC Report
Fri 10 Feb 2017
ERR1632397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145542
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5060.3476659658380399No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3470.23841915048577042No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3140.21574528314850694No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2220.15253328935977245No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2160.14841076802572453No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2120.1456624204696926No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1830.12573690068846108No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1810.12436272691044509No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1770.12161437935441315No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1680.1154305973533413No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1580.10855972846326145No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1530.10512429401822154No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1530.10512429401822154No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1460.10031468579516567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCTT200.001836657937.012
GGTATCA850.032.6470571
CTTAGGA303.584182E-430.8333342
ACTGCTT250.00548077529.5999988
TTAGCCA250.00548077529.5999984
ATAAGGC250.00548077529.5999983
CTTATAC856.730261E-1126.11764537
GGTAACG601.3240424E-624.66666825
TTAGGAC400.001923135223.1250023
AGGATAC400.001923135223.12500210
TTCTGCG652.656203E-622.7692318
GCGGTAA1500.022.19999923
TAGAACA502.6864174E-422.1999994
CAAGCCC603.6975747E-521.58333429
GAACAGA603.6975747E-521.5833346
CGCGGTA1550.021.48387122
CGGTAAT1550.021.48387124
GTATCAA2000.021.2749981
GGGTAAC705.0515755E-621.14285724
TGCGGGT705.0515755E-621.14285721
Produced by FastQC (version 0.11.5)