##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632397.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145542
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09595855491885	34.0	33.0	34.0	31.0	34.0
2	33.22879993403966	34.0	33.0	34.0	31.0	34.0
3	33.30861194706683	34.0	34.0	34.0	31.0	34.0
4	36.58436739910129	37.0	37.0	37.0	35.0	37.0
5	36.549538964697476	37.0	37.0	37.0	35.0	37.0
6	36.5883799865331	37.0	37.0	37.0	35.0	37.0
7	36.59099091671133	37.0	37.0	37.0	35.0	37.0
8	36.56285470860645	37.0	37.0	37.0	35.0	37.0
9	38.36016407634909	39.0	39.0	39.0	37.0	39.0
10	38.38445946874442	39.0	39.0	39.0	37.0	39.0
11	38.450701515713675	39.0	39.0	39.0	37.0	39.0
12	38.41625784996771	39.0	39.0	39.0	37.0	39.0
13	38.43954322463619	39.0	39.0	39.0	37.0	39.0
14	40.029132484093935	41.0	40.0	41.0	38.0	41.0
15	40.018482637314314	41.0	40.0	41.0	38.0	41.0
16	39.98546811229748	41.0	40.0	41.0	38.0	41.0
17	39.99072432699839	41.0	40.0	41.0	38.0	41.0
18	39.978844594687445	41.0	40.0	41.0	38.0	41.0
19	39.99070371439172	41.0	40.0	41.0	38.0	41.0
20	39.962828599304665	41.0	40.0	41.0	38.0	41.0
21	39.92909950392327	41.0	40.0	41.0	38.0	41.0
22	39.89855849170686	41.0	40.0	41.0	38.0	41.0
23	39.846387984224485	41.0	40.0	41.0	38.0	41.0
24	39.840417199159006	41.0	40.0	41.0	38.0	41.0
25	39.79165464264611	41.0	40.0	41.0	38.0	41.0
26	39.6978810240343	41.0	40.0	41.0	38.0	41.0
27	39.61849500487832	41.0	40.0	41.0	37.0	41.0
28	39.56837888719407	41.0	40.0	41.0	37.0	41.0
29	39.51640763490951	41.0	40.0	41.0	37.0	41.0
30	39.46873754655014	41.0	40.0	41.0	37.0	41.0
31	39.40329939124101	41.0	39.0	41.0	36.0	41.0
32	39.337778785505215	41.0	39.0	41.0	36.0	41.0
33	39.261202951725274	41.0	39.0	41.0	36.0	41.0
34	39.21936623105358	41.0	39.0	41.0	35.0	41.0
35	39.1228030396724	41.0	39.0	41.0	35.0	41.0
36	39.03910211485344	41.0	39.0	41.0	35.0	41.0
37	38.96800923444779	41.0	39.0	41.0	35.0	41.0
38	38.87814514023444	40.0	39.0	41.0	35.0	41.0
39	38.74086517981064	40.0	38.0	41.0	35.0	41.0
40	38.67486361325253	40.0	38.0	41.0	35.0	41.0
41	38.596480740954505	40.0	38.0	41.0	35.0	41.0
42	38.505290569045364	40.0	38.0	41.0	35.0	41.0
43	37.728270877135124	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	3.0
20	7.0
21	20.0
22	30.0
23	43.0
24	82.0
25	149.0
26	201.0
27	274.0
28	340.0
29	491.0
30	590.0
31	831.0
32	1076.0
33	1472.0
34	2057.0
35	3430.0
36	5665.0
37	10886.0
38	27941.0
39	89950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14092152093554	15.019032306825522	10.968655096123456	28.871391076115486
2	21.192508004562256	18.868780145937254	30.871501010017727	29.06721083948276
3	21.21518187189952	19.105825122645008	27.667614846573496	32.01137815888197
4	15.737725192727872	14.639760344093114	32.52188371741491	37.10063074576411
5	17.401162551016203	32.740377348119445	32.65243022632642	17.206029874537933
6	36.208104876942734	32.88191724725509	14.520894312294732	16.38908356350744
7	31.98939137843372	27.511646122768685	18.626925561006445	21.872036937791155
8	28.64946201096591	30.08409943521458	19.263168020227837	22.003270533591678
9	28.24889035467425	12.71866540242679	16.2736529661541	42.75879127674486
10	19.117505599758143	23.259265366698273	29.20943782550741	28.41379120803617
11	39.78851465556335	18.749914114138875	18.50805952920806	22.95351170108972
12	23.820615355017797	22.625084168143903	25.905924063157027	27.648376413681277
13	34.98028060628547	16.79377774113314	20.756894916931195	27.46904673565019
14	25.04569127811903	18.089623613802203	21.98197084003243	34.882714268046335
15	29.444421541548145	23.275068365145458	19.260419672671805	28.02009042063459
16	27.174973547154774	23.007104478432343	21.268774649242143	28.549147325170743
17	27.786480878371876	23.00160778332028	20.89087686028775	28.321034478020092
18	27.35361613829685	21.1100575778813	22.805788019953003	28.730538263868848
19	28.91811298456803	21.574528314850696	22.576988085913342	26.930370614667932
20	29.989968531420487	20.653831883579997	21.843866375341825	27.51233320965769
21	28.018716246856577	21.438485110827116	21.21243352434349	29.330365117972818
22	28.896813291008783	21.83149881133968	20.25051187973231	29.021176017919224
23	28.53884102183562	21.62468565774828	21.397946984375643	28.438526336040454
24	28.685190529194323	21.0757032334309	21.54567066551236	28.69343557186242
25	29.545423314232316	21.196630525896303	21.881656154237263	27.376290005634115
26	29.24928886506988	21.615066441302165	21.3168707314727	27.81877396215525
27	28.540215195613637	20.89706064228882	22.461557488560004	28.101166673537538
28	27.38453504830221	21.846614722897858	21.420620851712908	29.348229377087026
29	28.18293001332949	22.86075497107364	21.104560882769235	27.85175413282764
30	27.76655535859065	22.015638097593822	22.153742562284425	28.064063981531106
31	28.73603495898091	21.740803341990627	21.514064668617994	28.00909703041047
32	26.70912863640736	21.748361297769716	22.18260021162276	29.35990985420016
33	27.515081557213726	21.540861057289305	22.43888362122274	28.50517376427423
34	27.703343364801913	21.574528314850696	22.213519121628124	28.50860919871927
35	27.55836803122123	22.873809621964796	22.463618749227027	27.104203597586952
36	27.84076074260351	22.038999051820092	22.754943590166413	27.365296615409985
37	29.048659493479544	21.60269887730002	22.235505902076376	27.113135727144055
38	27.935578733286608	21.659727089087685	22.83808110373638	27.56661307388932
39	26.959915350895276	21.6349919610834	23.101922469115443	28.303170218905883
40	27.351554877629823	21.632930700416374	23.646782372098777	27.368732049855026
41	25.81522859380797	21.224114001456623	24.794904563631118	28.165752841104286
42	25.18516991658765	21.86241772134504	24.795591650520123	28.15682071154718
43	25.04637836500804	21.946242321804014	24.7591760454027	28.248203267785243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	5.0
7	8.0
8	6.0
9	4.0
10	13.0
11	22.0
12	22.0
13	33.0
14	44.0
15	87.0
16	130.0
17	122.5
18	115.0
19	115.0
20	133.0
21	151.0
22	139.0
23	127.0
24	163.5
25	200.0
26	200.0
27	249.0
28	298.0
29	430.5
30	563.0
31	680.0
32	797.0
33	797.0
34	1072.5
35	1348.0
36	1545.5
37	1743.0
38	2374.5
39	3006.0
40	3006.0
41	3666.0
42	4326.0
43	4870.5
44	5415.0
45	6936.5
46	8458.0
47	8458.0
48	9402.5
49	10347.0
50	12152.5
51	13958.0
52	15178.0
53	16398.0
54	16398.0
55	15270.0
56	14142.0
57	13532.0
58	12922.0
59	12365.0
60	11808.0
61	11808.0
62	11400.5
63	10993.0
64	9338.0
65	7683.0
66	6900.5
67	6118.0
68	6118.0
69	5386.0
70	4654.0
71	4112.5
72	3571.0
73	2978.0
74	2385.0
75	2385.0
76	1937.0
77	1489.0
78	1253.0
79	1017.0
80	782.0
81	547.0
82	547.0
83	463.5
84	380.0
85	288.0
86	196.0
87	144.0
88	92.0
89	92.0
90	75.5
91	59.0
92	37.0
93	15.0
94	10.5
95	6.0
96	6.0
97	3.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	145542.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.57810116667354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.03307223672759	54.20909428206291
2	5.755283300975207	7.087988347006362
3	1.8856977081520163	3.483530527270479
4	0.9573542210617928	2.358082203075401
5	0.6214991854678539	1.9135369858872355
6	0.4228872375086474	1.5624355856041554
7	0.30684430162236953	1.322642261340369
8	0.2599808082836803	1.280729961110882
9	0.19303296065698153	1.069794286185431
>10	1.4170627747651245	17.228016654986188
>50	0.10488496128182813	4.415220348765305
>100	0.04128450603646426	3.7212625908672408
>500	0.00111579746044498	0.3476659658380399
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	506	0.3476659658380399	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	347	0.23841915048577042	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	314	0.21574528314850694	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	222	0.15253328935977245	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	216	0.14841076802572453	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	212	0.1456624204696926	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	183	0.12573690068846108	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	181	0.12436272691044509	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	177	0.12161437935441315	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	168	0.1154305973533413	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	158	0.10855972846326145	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	153	0.10512429401822154	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	153	0.10512429401822154	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	146	0.10031468579516567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0013741737780159679	0.0
21	0.0	0.0	0.0	0.0027483475560319357	0.0
22	0.0	0.0	0.0	0.004809608223055888	0.0
23	0.0	0.0	0.0	0.005496695112063871	0.0
24	0.0	0.0	0.0	0.006870868890079839	0.0
25	0.0	0.0	0.0	0.007557955779087824	0.0
26	0.0	0.0	0.0	0.009619216446111776	0.0
27	0.0	0.0	0.0	0.0171771722251996	0.0
28	0.0	0.0	0.0	0.0515315166755988	0.0
29	0.0	0.0	0.0	0.09756633823913372	0.0
30	0.0	0.0	0.0	0.14497533358068462	0.0
31	0.0	0.0	0.0	0.32430501161176845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCCTT	20	0.0018366579	37.0	12
GGTATCA	85	0.0	32.647057	1
CTTAGGA	30	3.584182E-4	30.833334	2
ACTGCTT	25	0.005480775	29.599998	8
TTAGCCA	25	0.005480775	29.599998	4
ATAAGGC	25	0.005480775	29.599998	3
CTTATAC	85	6.730261E-11	26.117645	37
GGTAACG	60	1.3240424E-6	24.666668	25
TTAGGAC	40	0.0019231352	23.125002	3
AGGATAC	40	0.0019231352	23.125002	10
TTCTGCG	65	2.656203E-6	22.76923	18
GCGGTAA	150	0.0	22.199999	23
TAGAACA	50	2.6864174E-4	22.199999	4
CAAGCCC	60	3.6975747E-5	21.583334	29
GAACAGA	60	3.6975747E-5	21.583334	6
CGCGGTA	155	0.0	21.483871	22
CGGTAAT	155	0.0	21.483871	24
GTATCAA	200	0.0	21.274998	1
GGGTAAC	70	5.0515755E-6	21.142857	24
TGCGGGT	70	5.0515755E-6	21.142857	21
>>END_MODULE