FastQCFastQC Report
Fri 10 Feb 2017
ERR1632396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT140.4087591240875913TruSeq Adapter, Index 12 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA100.291970802919708No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC90.2627737226277372No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA80.23357664233576644No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA80.23357664233576644No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT80.23357664233576644No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT70.20437956204379565No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA70.20437956204379565No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT70.20437956204379565No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA70.20437956204379565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1751824817518248No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC60.1751824817518248No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT60.1751824817518248No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC60.1751824817518248No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC60.1751824817518248No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.1751824817518248No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA60.1751824817518248No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC50.145985401459854No Hit
AAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACG50.145985401459854No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA50.145985401459854No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC50.145985401459854No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA50.145985401459854No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC50.145985401459854RNA PCR Primer, Index 12 (95% over 23bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC50.145985401459854No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA50.145985401459854No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT50.145985401459854No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA50.145985401459854No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC50.145985401459854No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA50.145985401459854No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT40.11678832116788322No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA40.11678832116788322No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC40.11678832116788322No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG40.11678832116788322No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC40.11678832116788322No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG40.11678832116788322No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC40.11678832116788322No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA40.11678832116788322No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC40.11678832116788322No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT40.11678832116788322No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA40.11678832116788322No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT40.11678832116788322No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA40.11678832116788322No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG40.11678832116788322No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC40.11678832116788322No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC40.11678832116788322No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)