##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3425
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76729927007299	34.0	31.0	34.0	31.0	34.0
2	32.91445255474453	34.0	33.0	34.0	31.0	34.0
3	32.97080291970803	34.0	33.0	34.0	31.0	34.0
4	36.36613138686131	37.0	37.0	37.0	35.0	37.0
5	36.26744525547445	37.0	37.0	37.0	35.0	37.0
6	36.25897810218978	37.0	37.0	37.0	35.0	37.0
7	36.28	37.0	37.0	37.0	35.0	37.0
8	36.284087591240876	37.0	37.0	37.0	35.0	37.0
9	38.06335766423358	39.0	39.0	39.0	35.0	39.0
10	38.03941605839416	39.0	38.0	39.0	35.0	39.0
11	38.175766423357665	39.0	39.0	39.0	37.0	39.0
12	38.03270072992701	39.0	38.0	39.0	35.0	39.0
13	38.121751824817515	39.0	39.0	39.0	37.0	39.0
14	39.57109489051095	41.0	39.0	41.0	37.0	41.0
15	39.56058394160584	41.0	40.0	41.0	37.0	41.0
16	39.56992700729927	41.0	39.0	41.0	37.0	41.0
17	39.48554744525548	41.0	39.0	41.0	37.0	41.0
18	39.52233576642336	41.0	40.0	41.0	37.0	41.0
19	39.46890510948905	41.0	40.0	41.0	37.0	41.0
20	39.49138686131387	41.0	40.0	41.0	37.0	41.0
21	39.50978102189781	41.0	39.0	41.0	37.0	41.0
22	39.43211678832117	41.0	39.0	41.0	37.0	41.0
23	39.33751824817518	41.0	39.0	41.0	36.0	41.0
24	39.32583941605839	41.0	39.0	41.0	36.0	41.0
25	39.23299270072993	41.0	39.0	41.0	35.0	41.0
26	39.08437956204379	41.0	39.0	41.0	35.0	41.0
27	39.0178102189781	41.0	39.0	41.0	35.0	41.0
28	38.9614598540146	40.0	39.0	41.0	35.0	41.0
29	38.9243795620438	40.0	39.0	41.0	35.0	41.0
30	38.88204379562044	40.0	39.0	41.0	35.0	41.0
31	38.871532846715326	40.0	38.0	41.0	35.0	41.0
32	38.785401459854015	40.0	38.0	41.0	35.0	41.0
33	38.72525547445255	40.0	38.0	41.0	35.0	41.0
34	38.68087591240876	40.0	38.0	41.0	35.0	41.0
35	38.614890510948904	40.0	38.0	41.0	35.0	41.0
36	38.558832116788324	40.0	38.0	41.0	35.0	41.0
37	38.50131386861314	40.0	38.0	41.0	35.0	41.0
38	38.31912408759124	40.0	38.0	41.0	34.0	41.0
39	38.31824817518248	40.0	38.0	41.0	34.0	41.0
40	38.21897810218978	40.0	38.0	41.0	34.0	41.0
41	38.25751824817518	40.0	37.0	41.0	34.0	41.0
42	38.13430656934307	40.0	37.0	41.0	34.0	41.0
43	37.377518248175186	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	4.0
24	4.0
25	8.0
26	8.0
27	12.0
28	27.0
29	23.0
30	19.0
31	38.0
32	44.0
33	61.0
34	75.0
35	122.0
36	157.0
37	307.0
38	621.0
39	1894.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.54014598540146	15.153284671532846	12.613138686131386	29.693430656934304
2	21.16788321167883	18.89051094890511	31.59124087591241	28.35036496350365
3	20.583941605839414	19.065693430656935	28.934306569343065	31.416058394160583
4	15.270072992700731	16.67153284671533	33.43065693430657	34.627737226277375
5	17.927007299270073	32.175182481751825	32.46715328467153	17.43065693430657
6	35.12408759124088	32.058394160583944	15.328467153284672	17.489051094890513
7	30.423357664233574	27.532846715328468	18.77372262773723	23.27007299270073
8	27.708029197080293	30.18978102189781	19.766423357664234	22.335766423357665
9	26.94890510948905	12.554744525547445	16.554744525547445	43.941605839416056
10	18.364963503649633	23.124087591240876	28.291970802919707	30.21897810218978
11	40.379562043795616	19.065693430656935	17.401459854014597	23.153284671532848
12	22.978102189781023	23.67883211678832	26.1021897810219	27.240875912408757
13	36.75912408759124	16.525547445255476	20.37956204379562	26.335766423357665
14	24.817518248175183	18.1021897810219	22.89051094890511	34.18978102189781
15	30.56934306569343	22.62773722627737	19.153284671532848	27.64963503649635
16	26.5985401459854	24.175182481751825	20.934306569343068	28.291970802919707
17	27.766423357664234	24.02919708029197	20.554744525547445	27.64963503649635
18	28.525547445255473	19.503649635036496	22.131386861313867	29.839416058394157
19	29.22627737226277	22.16058394160584	21.547445255474454	27.065693430656935
20	30.423357664233574	20.145985401459853	22.656934306569344	26.77372262773723
21	29.985401459854018	17.722627737226276	22.423357664233574	29.86861313868613
22	28.846715328467155	22.773722627737225	20.67153284671533	27.708029197080293
23	28.64233576642336	20.583941605839414	22.014598540145986	28.759124087591243
24	29.547445255474454	21.255474452554747	20.525547445255473	28.67153284671533
25	27.795620437956202	21.576642335766426	22.277372262773724	28.35036496350365
26	27.795620437956202	21.45985401459854	21.722627737226276	29.021897810218977
27	27.27007299270073	21.927007299270073	23.357664233576642	27.44525547445256
28	26.8029197080292	22.744525547445253	21.868613138686133	28.583941605839414
29	26.394160583941606	23.153284671532848	21.021897810218977	29.43065693430657
30	26.54014598540146	21.956204379562045	22.306569343065693	29.1970802919708
31	27.795620437956202	22.218978102189784	21.343065693430656	28.64233576642336
32	27.036496350364963	20.817518248175183	22.802919708029197	29.34306569343066
33	26.744525547445257	22.131386861313867	23.24087591240876	27.883211678832115
34	27.211678832116785	20.934306569343068	22.744525547445253	29.10948905109489
35	26.043795620437955	23.21167883211679	24.204379562043794	26.54014598540146
36	25.576642335766426	22.335766423357665	24.846715328467152	27.240875912408757
37	27.64963503649635	20.467153284671532	24.992700729927005	26.89051094890511
38	25.401459854014597	20.67153284671533	25.05109489051095	28.875912408759124
39	24.875912408759124	20.0	25.372262773722625	29.751824817518248
40	24.0	20.817518248175183	27.532846715328468	27.64963503649635
41	22.773722627737225	18.97810218978102	28.905109489051096	29.34306569343066
42	21.81021897810219	19.73722627737226	29.05109489051095	29.4014598540146
43	20.087591240875913	20.35036496350365	30.1021897810219	29.45985401459854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.5
11	3.0
12	3.0
13	3.5
14	4.0
15	6.5
16	9.0
17	7.0
18	5.0
19	5.0
20	4.5
21	4.0
22	4.0
23	4.0
24	6.0
25	8.0
26	8.0
27	8.5
28	9.0
29	10.5
30	12.0
31	16.0
32	20.0
33	20.0
34	19.5
35	19.0
36	27.5
37	36.0
38	43.0
39	50.0
40	50.0
41	63.5
42	77.0
43	98.0
44	119.0
45	173.5
46	228.0
47	228.0
48	252.5
49	277.0
50	325.5
51	374.0
52	380.5
53	387.0
54	387.0
55	367.5
56	348.0
57	340.5
58	333.0
59	314.5
60	296.0
61	296.0
62	276.5
63	257.0
64	212.0
65	167.0
66	148.0
67	129.0
68	129.0
69	103.5
70	78.0
71	73.5
72	69.0
73	54.0
74	39.0
75	39.0
76	32.5
77	26.0
78	20.5
79	15.0
80	11.5
81	8.0
82	8.0
83	8.0
84	8.0
85	5.0
86	2.0
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	1.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3425.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.08759124087592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.77427908518395	81.72262773722628
2	4.772953264832616	8.40875912408759
3	0.9612197547232351	2.54014598540146
4	0.5303281405369572	1.8686131386861315
5	0.39774610540271793	1.7518248175182483
6	0.23201856148491878	1.2262773722627736
7	0.1325820351342393	0.8175182481751826
8	0.09943652635067948	0.7007299270072992
9	0.033145508783559825	0.2627737226277372
>10	0.06629101756711965	0.7007299270072992
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	14	0.4087591240875913	TruSeq Adapter, Index 12 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	10	0.291970802919708	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	9	0.2627737226277372	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	8	0.23357664233576644	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	8	0.23357664233576644	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	8	0.23357664233576644	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	7	0.20437956204379565	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	7	0.20437956204379565	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	7	0.20437956204379565	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	7	0.20437956204379565	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.1751824817518248	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	6	0.1751824817518248	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	6	0.1751824817518248	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	6	0.1751824817518248	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	6	0.1751824817518248	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	6	0.1751824817518248	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	6	0.1751824817518248	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	5	0.145985401459854	No Hit
AAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACG	5	0.145985401459854	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	5	0.145985401459854	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	5	0.145985401459854	No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA	5	0.145985401459854	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	5	0.145985401459854	RNA PCR Primer, Index 12 (95% over 23bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	5	0.145985401459854	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	5	0.145985401459854	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	5	0.145985401459854	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	5	0.145985401459854	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	5	0.145985401459854	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	5	0.145985401459854	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	4	0.11678832116788322	No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA	4	0.11678832116788322	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	4	0.11678832116788322	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	4	0.11678832116788322	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	4	0.11678832116788322	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	4	0.11678832116788322	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	4	0.11678832116788322	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	4	0.11678832116788322	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	4	0.11678832116788322	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	4	0.11678832116788322	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	4	0.11678832116788322	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	4	0.11678832116788322	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	4	0.11678832116788322	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	4	0.11678832116788322	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	4	0.11678832116788322	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	4	0.11678832116788322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.029197080291970802	0.0
27	0.0	0.0	0.0	0.029197080291970802	0.0
28	0.0	0.0	0.0	0.058394160583941604	0.0
29	0.0	0.0	0.0	0.23357664233576642	0.0
30	0.0	0.0	0.0	0.5547445255474452	0.0
31	0.0	0.0	0.0	0.9635036496350365	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE