FastQCFastQC Report
Fri 10 Feb 2017
ERR1632395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18156
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA340.18726591760299627No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC270.14871116986120292No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA240.13218770654329146No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA230.126679885437321No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC220.12117206433135053No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT200.11015642211940956No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.11015642211940956No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC190.10464860101343909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303.4630083E-430.8333321
TCTTATA658.374991E-825.61538537
GTATCAA400.001859321523.1252
AACGCAG500.00677797618.57
ACGCAGA500.00677797618.58
TCAACGC500.00677797618.55
CAGAGTA500.00677797618.511
AGAGTAC500.00677797618.512
GCAGAGT500.00677797618.510
TATCAAC500.00677797618.53
ATCAACG500.00677797618.54
CAACGCA500.00677797618.56
CTCTTAT1058.373749E-615.85714236
TCTCTTA1602.305831E-612.7187537
GCTGTCT1309.224991E-411.38461537
GTCTCTT2053.634392E-59.9268336
TGTCTCT2553.9220767E-47.98039235
CTGTCTC2800.00106854697.267857634
Produced by FastQC (version 0.11.5)