##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18156
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03888521700815	34.0	33.0	34.0	31.0	34.0
2	33.17938973342146	34.0	33.0	34.0	31.0	34.0
3	33.23479841374752	34.0	34.0	34.0	31.0	34.0
4	36.5408680326063	37.0	37.0	37.0	35.0	37.0
5	36.52197620621282	37.0	37.0	37.0	35.0	37.0
6	36.549019607843135	37.0	37.0	37.0	35.0	37.0
7	36.54610046265697	37.0	37.0	37.0	35.0	37.0
8	36.52759418374091	37.0	37.0	37.0	35.0	37.0
9	38.31444150693986	39.0	39.0	39.0	37.0	39.0
10	38.344404053756335	39.0	39.0	39.0	37.0	39.0
11	38.41468385106852	39.0	39.0	39.0	37.0	39.0
12	38.37568847763825	39.0	39.0	39.0	37.0	39.0
13	38.418759638686936	39.0	39.0	39.0	37.0	39.0
14	39.96552103987663	41.0	40.0	41.0	38.0	41.0
15	39.95670852610707	41.0	40.0	41.0	38.0	41.0
16	39.903227583168096	41.0	40.0	41.0	38.0	41.0
17	39.907854152897116	41.0	40.0	41.0	38.0	41.0
18	39.907854152897116	41.0	40.0	41.0	38.0	41.0
19	39.92355144304913	41.0	40.0	41.0	38.0	41.0
20	39.9035029742234	41.0	40.0	41.0	38.0	41.0
21	39.88863185723728	41.0	40.0	41.0	38.0	41.0
22	39.8396673276052	41.0	40.0	41.0	38.0	41.0
23	39.79841374752148	41.0	40.0	41.0	38.0	41.0
24	39.77864066975105	41.0	40.0	41.0	38.0	41.0
25	39.74399647499449	41.0	40.0	41.0	38.0	41.0
26	39.6571932143644	41.0	40.0	41.0	37.0	41.0
27	39.6010134390835	41.0	40.0	41.0	37.0	41.0
28	39.56730557391496	41.0	40.0	41.0	37.0	41.0
29	39.52357347433355	41.0	40.0	41.0	37.0	41.0
30	39.481493721083936	41.0	40.0	41.0	37.0	41.0
31	39.39353381802159	41.0	39.0	41.0	36.0	41.0
32	39.362965410883454	41.0	39.0	41.0	36.0	41.0
33	39.3093192333113	41.0	39.0	41.0	36.0	41.0
34	39.23347653668209	41.0	39.0	41.0	35.0	41.0
35	39.193104207975324	41.0	39.0	41.0	35.0	41.0
36	39.13389513108614	41.0	39.0	41.0	35.0	41.0
37	39.078045825071605	41.0	39.0	41.0	35.0	41.0
38	38.99977968715576	41.0	39.0	41.0	35.0	41.0
39	38.92360652126019	40.0	39.0	41.0	35.0	41.0
40	38.81124697069839	40.0	39.0	41.0	35.0	41.0
41	38.76140118968936	40.0	38.0	41.0	35.0	41.0
42	38.685283102004846	40.0	38.0	41.0	35.0	41.0
43	37.975545274289495	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	1.0
22	4.0
23	6.0
24	10.0
25	20.0
26	18.0
27	25.0
28	42.0
29	61.0
30	93.0
31	94.0
32	133.0
33	196.0
34	249.0
35	479.0
36	672.0
37	1353.0
38	3421.0
39	11276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75170742454285	16.402291253580085	12.574355584930602	28.271645736946464
2	21.5300727032386	19.25534258647279	32.04450319453624	27.17008151575237
3	20.6157743996475	19.877726371447455	29.081295439524123	30.425203789380923
4	15.432914738929279	15.846001321877065	32.71094954835867	36.010134390834985
5	16.831901299845782	33.04692663582287	33.239700374531836	16.881471689799515
6	35.36571932143644	32.887199823749725	14.84908570169641	16.897995153117424
7	30.138797091870455	28.359770874641992	19.073584489975765	22.427847543511785
8	27.91363736505838	31.11368142762723	19.39854593522802	21.574135272086362
9	27.836527869574795	12.712051112579864	17.26701916721745	42.18440185062789
10	19.34346772416832	23.887420136593963	29.571491517955494	27.19762062128222
11	39.00088125137696	19.11764705882353	18.710068296981714	23.1714033928178
12	23.254020709407357	23.50187265917603	25.82617316589557	27.41793346552104
13	34.93060145406478	17.59198061246971	21.11698612029081	26.360431813174706
14	24.790702797973122	19.32143643974444	22.54901960784314	33.3388411544393
15	29.04274069178233	23.83784974664023	19.778585591539986	27.340823970037455
16	26.756994932804584	23.29808327825512	21.706322978629654	28.238598810310638
17	27.379378717779247	23.689138576779026	21.260189469046047	27.671293236395684
18	26.233751927737387	21.706322978629654	22.973121833002864	29.086803260630095
19	29.7202026878167	22.191011235955056	21.65675258867592	26.432033487552324
20	29.588014981273407	21.072923551443047	22.168979951531174	27.17008151575237
21	27.952192112800176	22.191011235955056	21.012337519277374	28.844459131967394
22	28.756333994271866	22.951090548578982	20.665344789601235	27.62723066754792
23	27.709847984137475	22.45538664904164	22.014760960564	27.82000440625689
24	28.073364177131527	21.700815157523685	22.091870456047587	28.133950209297204
25	28.166997135933023	21.99823749724609	23.25952853051333	26.57523683630756
26	28.095395461555412	22.444371006829698	22.433355364617757	27.026878166997136
27	26.872659176029963	21.585150914298303	23.97554527428949	27.566644635382243
28	26.200705001101564	22.984137475214805	21.954174928398327	28.860982595285307
29	26.674377616215022	23.46331791143424	22.020268781669973	27.842035690680767
30	27.115003304692664	22.538003965631194	23.35316148931483	26.993831240361317
31	27.137034589116542	22.681207314386427	22.504957038995375	27.676801057501656
32	25.3359770874642	22.46089447014761	22.851949768671513	29.351178673716678
33	25.82617316589557	21.474994492178894	24.66953073364177	28.029301608283763
34	26.525666446353824	21.342806785635602	24.449217889402952	27.682308878607625
35	25.90328266137916	22.54901960784314	24.7576558713373	26.790041859440407
36	26.228244106631415	21.06190790923111	25.214805023132847	27.49504296100463
37	25.50121172064331	21.056400088125137	25.55628993170302	27.88609825952853
38	25.198281559814937	20.63229786296541	26.432033487552324	27.737387089667326
39	24.272967614011897	20.04296100462657	27.58867591980613	28.095395461555412
40	24.350077109495484	20.070500110156424	28.54152897113902	27.03789380920908
41	22.884996695307336	19.48116325181758	29.75324961445252	27.88059043842256
42	21.260189469046047	20.918704560475877	29.85239039435999	27.968715576118086
43	20.54417272526988	20.764485569508704	29.758757435558493	28.932584269662918
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.0
10	2.5
11	3.0
12	3.0
13	6.0
14	9.0
15	15.5
16	22.0
17	24.0
18	26.0
19	26.0
20	30.5
21	35.0
22	28.0
23	21.0
24	28.0
25	35.0
26	35.0
27	45.5
28	56.0
29	71.5
30	87.0
31	108.0
32	129.0
33	129.0
34	177.5
35	226.0
36	252.0
37	278.0
38	368.5
39	459.0
40	459.0
41	524.0
42	589.0
43	709.5
44	830.0
45	1014.0
46	1198.0
47	1198.0
48	1383.0
49	1568.0
50	1709.5
51	1851.0
52	1969.0
53	2087.0
54	2087.0
55	1968.5
56	1850.0
57	1750.5
58	1651.0
59	1542.5
60	1434.0
61	1434.0
62	1304.0
63	1174.0
64	978.5
65	783.0
66	685.5
67	588.0
68	588.0
69	493.5
70	399.0
71	346.5
72	294.0
73	237.0
74	180.0
75	180.0
76	146.5
77	113.0
78	94.0
79	75.0
80	60.0
81	45.0
82	45.0
83	36.5
84	28.0
85	20.0
86	12.0
87	11.0
88	10.0
89	10.0
90	7.0
91	4.0
92	3.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	18156.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.05001101564221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.25978384909713	74.05816259087905
2	5.658857488084849	9.286186384666225
3	1.8460092636101229	4.543952412425645
4	0.8055313150298717	2.6437541308658297
5	0.5370208766865812	2.2031284423881914
6	0.22152111163321475	1.0905485789821545
7	0.12754245821306304	0.7325402070940736
8	0.14096798013022757	0.9253139458030404
9	0.07384037054440491	0.5452742894910773
>10	0.328925286970531	3.9711390174047145
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	34	0.18726591760299627	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	27	0.14871116986120292	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	24	0.13218770654329146	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	23	0.126679885437321	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	22	0.12117206433135053	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	20	0.11015642211940956	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	20	0.11015642211940956	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	19	0.10464860101343909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0055078211059704785	0.0
19	0.0	0.0	0.0	0.0055078211059704785	0.0
20	0.0	0.0	0.0	0.0055078211059704785	0.0
21	0.0	0.0	0.0	0.0055078211059704785	0.0
22	0.0	0.0	0.0	0.0055078211059704785	0.0
23	0.0	0.0	0.0	0.0055078211059704785	0.0
24	0.0	0.0	0.0	0.0055078211059704785	0.0
25	0.0	0.0	0.0	0.0055078211059704785	0.0
26	0.0	0.0	0.0	0.03304692663582287	0.0
27	0.0	0.0	0.0	0.08812513769552766	0.0
28	0.0	0.0	0.0	0.253359770874642	0.0
29	0.0	0.0	0.0	0.4736726151134611	0.0
30	0.0	0.0	0.0	0.7876184181537783	0.0
31	0.0	0.0	0.0	1.6192994051553204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	3.4630083E-4	30.833332	1
TCTTATA	65	8.374991E-8	25.615385	37
GTATCAA	40	0.0018593215	23.125	2
AACGCAG	50	0.006777976	18.5	7
ACGCAGA	50	0.006777976	18.5	8
TCAACGC	50	0.006777976	18.5	5
CAGAGTA	50	0.006777976	18.5	11
AGAGTAC	50	0.006777976	18.5	12
GCAGAGT	50	0.006777976	18.5	10
TATCAAC	50	0.006777976	18.5	3
ATCAACG	50	0.006777976	18.5	4
CAACGCA	50	0.006777976	18.5	6
CTCTTAT	105	8.373749E-6	15.857142	36
TCTCTTA	160	2.305831E-6	12.71875	37
GCTGTCT	130	9.224991E-4	11.384615	37
GTCTCTT	205	3.634392E-5	9.92683	36
TGTCTCT	255	3.9220767E-4	7.980392	35
CTGTCTC	280	0.0010685469	7.2678576	34
>>END_MODULE