FastQCFastQC Report
Fri 10 Feb 2017
ERR1632394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34352
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT13103.813460642757336TruSeq Adapter, Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC5011.4584303679552866TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC4351.266301816488123No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC3410.9926641825803446RNA PCR Primer, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT3390.9868421052631579No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG2930.8529343269678622No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG1300.3784350256171402No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA850.24743828598043782No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA770.22414997671169073No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC700.20377270610153703No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA630.18339543549138332No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT620.18048439683278994No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA610.17757335817419656No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC580.1688402421984164No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA560.16301816488122964No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT540.15719608756404285No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT540.15719608756404285No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG520.15137401024685607No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA520.15137401024685607No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA520.15137401024685607No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC480.13972985561248255No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA460.13390777829529577No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT460.13390777829529577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT430.1251746623195156No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC420.12226362366092222No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA420.12226362366092222No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT410.11935258500232883No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT400.11644154634373545No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG400.11644154634373545No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA400.11644154634373545No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG390.11353050768514204No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC390.11353050768514204No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC390.11353050768514204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT380.11061946902654868No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT370.1077084303679553TruSeq Adapter, Index 11 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG370.1077084303679553No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC370.1077084303679553No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT360.10479739170936189No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC360.10479739170936189No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC350.10188635305076851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC401.5411024E-632.37537
GCCGTCT2100.030.83333236
GGTATCA551.7475031E-830.2727281
CCGTCTT2150.030.11628237
ATGCCGT2700.023.9814834
TGCCGTC2750.023.54545635
TATACAC802.5363988E-823.1253
TTATACA802.5363988E-823.1252
TGGACAG400.001893514923.1255
GTATCAA753.5273115E-722.2000032
TATGCCG2950.021.94915233
TACGGGA450.00375188220.55555716
TACACAT1001.1841621E-820.3499985
GTATGCC3250.019.92307732
CGTATGC3250.019.92307731
TATCAAC1002.6790076E-718.4999983
ATACACA1002.6790076E-718.4999984
TCGTATG3600.017.98611330
CTCGTAT3650.017.73972529
TCTCGTA3700.017.528
Produced by FastQC (version 0.11.5)