##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632394.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34352
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0039299021891	34.0	33.0	34.0	31.0	34.0
2	33.15675943176525	34.0	33.0	34.0	31.0	34.0
3	33.24411970190964	34.0	34.0	34.0	31.0	34.0
4	36.532458081043316	37.0	37.0	37.0	35.0	37.0
5	36.484891709361904	37.0	37.0	37.0	35.0	37.0
6	36.53801816488123	37.0	37.0	37.0	35.0	37.0
7	36.511847927340476	37.0	37.0	37.0	35.0	37.0
8	36.5072484862599	37.0	37.0	37.0	35.0	37.0
9	38.309326967862134	39.0	39.0	39.0	37.0	39.0
10	38.34213437354448	39.0	39.0	39.0	37.0	39.0
11	38.396250582207735	39.0	39.0	39.0	37.0	39.0
12	38.31605146716348	39.0	39.0	39.0	37.0	39.0
13	38.38792501164416	39.0	39.0	39.0	37.0	39.0
14	39.876047973917096	41.0	40.0	41.0	38.0	41.0
15	39.87828947368421	41.0	40.0	41.0	38.0	41.0
16	39.88131695388915	41.0	40.0	41.0	38.0	41.0
17	39.817128551467164	41.0	40.0	41.0	38.0	41.0
18	39.88594550535631	41.0	40.0	41.0	38.0	41.0
19	39.85890195621798	41.0	40.0	41.0	38.0	41.0
20	39.81814741499767	41.0	40.0	41.0	38.0	41.0
21	39.762837680484395	41.0	40.0	41.0	38.0	41.0
22	39.67664182580344	41.0	40.0	41.0	38.0	41.0
23	39.696407778295296	41.0	40.0	41.0	38.0	41.0
24	39.66982999534234	41.0	40.0	41.0	38.0	41.0
25	39.64293199813694	41.0	40.0	41.0	37.0	41.0
26	39.590620633442015	41.0	40.0	41.0	37.0	41.0
27	39.46218560782487	41.0	39.0	41.0	37.0	41.0
28	39.47633325570563	41.0	39.0	41.0	37.0	41.0
29	39.42716581276199	41.0	39.0	41.0	37.0	41.0
30	39.41063111318118	41.0	39.0	41.0	37.0	41.0
31	39.32690964136004	41.0	39.0	41.0	36.0	41.0
32	39.23445505356311	41.0	39.0	41.0	36.0	41.0
33	39.14598858872846	41.0	39.0	41.0	35.0	41.0
34	39.11681998136935	41.0	39.0	41.0	35.0	41.0
35	39.057405682347465	40.0	39.0	41.0	35.0	41.0
36	39.00666627852818	40.0	39.0	41.0	35.0	41.0
37	38.97906963204471	40.0	39.0	41.0	35.0	41.0
38	38.89721122496507	40.0	39.0	41.0	35.0	41.0
39	38.76280857009781	40.0	38.0	41.0	35.0	41.0
40	38.618217279925474	40.0	38.0	41.0	35.0	41.0
41	38.6136178388449	40.0	38.0	41.0	35.0	41.0
42	38.55891942244993	40.0	38.0	41.0	35.0	41.0
43	37.85176991150443	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	2.0
21	5.0
22	9.0
23	12.0
24	25.0
25	29.0
26	37.0
27	66.0
28	98.0
29	115.0
30	143.0
31	223.0
32	263.0
33	409.0
34	577.0
35	849.0
36	1387.0
37	2862.0
38	7042.0
39	20197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.4387517466232	16.654052165812764	15.367373078714486	28.539823008849556
2	19.535980437820214	19.2332324173265	31.206334420121102	30.024452724732186
3	19.151723334885887	18.927573358174197	32.6589427107592	29.261760596180718
4	13.05891942244993	15.445971122496507	30.985095482068004	40.51001397298556
5	15.894271075919889	31.95738239403819	32.947135537959944	19.201210992081975
6	35.31089892873777	32.23684210526316	15.085002328830926	17.36725663716814
7	28.67081974848626	29.194806707033074	17.457498835584538	24.676874708896136
8	30.61539357242664	28.100256171401956	20.56357708430368	20.720773171867723
9	25.582207731718675	11.990568234746156	14.852119236143457	47.57510479739171
10	18.78493246390312	21.640661387983233	27.069748486259897	32.50465766185375
11	38.01234280391243	18.240568234746156	16.75593851886353	26.991150442477874
12	21.116674429436422	26.40020959478342	22.982650209594784	29.500465766185375
13	36.868304611085236	14.82009781089893	18.52585002328831	29.785747554727525
14	26.304145319049837	20.668374476013042	20.46169073125291	32.56578947368421
15	28.525267815556592	24.94177922682813	17.77771308802981	28.75523986958547
16	30.350489054494645	22.449930135072194	19.600023288309266	27.599557522123895
17	25.570563577084304	27.82079646017699	18.377387051700047	28.231252911038656
18	28.644620400558917	18.234746157428972	19.760130414531904	33.360503027480206
19	33.41290172333488	22.260712622263622	19.209944108057755	25.116441546343736
20	33.19748486259898	20.260829063809968	18.776199347927342	27.765486725663717
21	30.362133209129016	23.50663716814159	18.33663251047974	27.794597112249654
22	32.49301350721937	22.918607359105728	18.074639031206335	26.513740102468557
23	26.732068001863063	20.9070796460177	20.310316721006057	32.05053563111318
24	29.53830926874709	20.930367955286446	23.413483931066605	26.11783884489986
25	27.3754075454122	24.100489054494645	20.40929203539823	28.114811364694926
26	28.04785747554728	22.479040521658128	20.56066604564509	28.912435957149512
27	27.093036795528647	25.180484396832792	21.9201210992082	25.806357708430365
28	24.155798789007918	21.582440614811365	23.139846297158826	31.121914299021892
29	27.785863996273868	22.391709361900325	22.088961341406613	27.73346530041919
30	26.394387517466235	20.173497904052166	25.168840242198414	28.263274336283185
31	25.983931066604566	20.65381928272007	21.084653004191896	32.27759664648347
32	28.114811364694926	21.75128085700978	23.13402421984164	26.999883558453657
33	23.93455985095482	20.129832324173265	26.935840707964605	28.99976711690731
34	26.053795994410805	24.077200745225895	23.524103400093153	26.344899860270143
35	24.694340940847695	21.649394503959012	28.90370284117373	24.75256171401956
36	27.058104331625525	21.2156497438286	22.155915230554264	29.570330693991615
37	24.88064741499767	20.83721471821146	24.839892873777362	29.442244993013507
38	25.657894736842106	19.364229156963205	24.720540288775034	30.257335817419655
39	27.1570796460177	19.760130414531904	25.206683744760127	27.876106194690266
40	22.930251513740103	18.86061946902655	31.84094084769446	26.368188169538893
41	23.308686539357243	19.15463437354448	26.48754075454122	31.049138332557057
42	19.955170004657663	18.86353050768514	33.974732184443404	27.206567303213784
43	19.917326502095946	18.80239869585468	30.824988355845367	30.455286446204006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	4.0
6	3.5
7	3.0
8	2.5
9	2.0
10	4.5
11	7.0
12	7.0
13	13.0
14	19.0
15	41.0
16	63.0
17	58.0
18	53.0
19	53.0
20	66.0
21	79.0
22	65.0
23	51.0
24	45.5
25	40.0
26	40.0
27	47.0
28	54.0
29	65.5
30	77.0
31	89.5
32	102.0
33	102.0
34	179.0
35	256.0
36	283.0
37	310.0
38	414.5
39	519.0
40	519.0
41	604.5
42	690.0
43	897.5
44	1105.0
45	1630.0
46	2155.0
47	2155.0
48	2406.5
49	2658.0
50	3003.0
51	3348.0
52	3820.0
53	4292.0
54	4292.0
55	3679.0
56	3066.0
57	3807.5
58	4549.0
59	4145.0
60	3741.0
61	3741.0
62	3020.5
63	2300.0
64	1813.0
65	1326.0
66	1214.5
67	1103.0
68	1103.0
69	964.5
70	826.0
71	811.0
72	796.0
73	629.0
74	462.0
75	462.0
76	298.0
77	134.0
78	101.5
79	69.0
80	55.0
81	41.0
82	41.0
83	35.0
84	29.0
85	19.0
86	9.0
87	8.0
88	7.0
89	7.0
90	4.5
91	2.0
92	2.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	34352.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.9981369352585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.70377936670072	48.279576152771305
2	7.068437180796732	8.057755006986493
3	2.635342185903984	4.506287843502562
4	1.4402451481103167	3.28365160689334
5	0.9397344228804902	2.6781555659059153
6	0.5873340143003064	2.0086166744294367
7	0.5362614913176711	2.139613414066139
8	0.39325842696629215	1.7931998136935259
9	0.2655771195097038	1.3623660922217047
>10	1.3278855975485189	13.82452258965999
>50	0.06639427987742595	2.3171867722403356
>100	0.02553626149131767	4.477177456916627
>500	0.005107252298263534	1.4584303679552866
>1k	0.005107252298263534	3.813460642757336
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	1310	3.813460642757336	TruSeq Adapter, Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	501	1.4584303679552866	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	435	1.266301816488123	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	341	0.9926641825803446	RNA PCR Primer, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	339	0.9868421052631579	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	293	0.8529343269678622	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	130	0.3784350256171402	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	85	0.24743828598043782	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	77	0.22414997671169073	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	70	0.20377270610153703	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	63	0.18339543549138332	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	62	0.18048439683278994	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	61	0.17757335817419656	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	58	0.1688402421984164	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	56	0.16301816488122964	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	54	0.15719608756404285	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	54	0.15719608756404285	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	52	0.15137401024685607	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	52	0.15137401024685607	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	52	0.15137401024685607	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	48	0.13972985561248255	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	46	0.13390777829529577	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	46	0.13390777829529577	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	43	0.1251746623195156	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	42	0.12226362366092222	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	42	0.12226362366092222	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	41	0.11935258500232883	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	40	0.11644154634373545	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	40	0.11644154634373545	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	40	0.11644154634373545	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	39	0.11353050768514204	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	39	0.11353050768514204	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	39	0.11353050768514204	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.11061946902654868	No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT	37	0.1077084303679553	TruSeq Adapter, Index 11 (95% over 22bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	37	0.1077084303679553	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	37	0.1077084303679553	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	36	0.10479739170936189	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	36	0.10479739170936189	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	35	0.10188635305076851	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0029110386585933863	0.0	0.0	0.0	0.0
9	0.0029110386585933863	0.0	0.0	0.0	0.0
10	0.0029110386585933863	0.0	0.0	0.0	0.0
11	0.0029110386585933863	0.0	0.0	0.0	0.0
12	0.0029110386585933863	0.0	0.0	0.0	0.0
13	0.0029110386585933863	0.0	0.0	0.0	0.0
14	0.0029110386585933863	0.0	0.0	0.0	0.0
15	0.0029110386585933863	0.0	0.0	0.0	0.0
16	0.0029110386585933863	0.0	0.0	0.0	0.0
17	0.0029110386585933863	0.0	0.0	0.0	0.0
18	0.0029110386585933863	0.0	0.0	0.0029110386585933863	0.0
19	0.0029110386585933863	0.0	0.0	0.005822077317186773	0.0
20	0.0029110386585933863	0.0	0.0	0.005822077317186773	0.0
21	0.0029110386585933863	0.0	0.0	0.014555193292966931	0.0
22	0.0029110386585933863	0.0	0.0	0.017466231951560315	0.0
23	0.0029110386585933863	0.0	0.0	0.02328830926874709	0.0
24	0.0029110386585933863	0.0	0.0	0.02328830926874709	0.0
25	0.0029110386585933863	0.0	0.0	0.02328830926874709	0.0
26	0.0029110386585933863	0.0	0.0	0.026199347927340477	0.0
27	0.0029110386585933863	0.0	0.0	0.05530973451327434	0.0
28	0.0029110386585933863	0.0	0.0	0.21832789939450395	0.0
29	0.0029110386585933863	0.0	0.0	0.4774103400093153	0.0
30	0.0029110386585933863	0.0	0.0	0.7248486259897532	0.0
31	0.0029110386585933863	0.0	0.0	1.3769212855146717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	40	1.5411024E-6	32.375	37
GCCGTCT	210	0.0	30.833332	36
GGTATCA	55	1.7475031E-8	30.272728	1
CCGTCTT	215	0.0	30.116282	37
ATGCCGT	270	0.0	23.98148	34
TGCCGTC	275	0.0	23.545456	35
TATACAC	80	2.5363988E-8	23.125	3
TTATACA	80	2.5363988E-8	23.125	2
TGGACAG	40	0.0018935149	23.125	5
GTATCAA	75	3.5273115E-7	22.200003	2
TATGCCG	295	0.0	21.949152	33
TACGGGA	45	0.003751882	20.555557	16
TACACAT	100	1.1841621E-8	20.349998	5
GTATGCC	325	0.0	19.923077	32
CGTATGC	325	0.0	19.923077	31
TATCAAC	100	2.6790076E-7	18.499998	3
ATACACA	100	2.6790076E-7	18.499998	4
TCGTATG	360	0.0	17.986113	30
CTCGTAT	365	0.0	17.739725	29
TCTCGTA	370	0.0	17.5	28
>>END_MODULE