FastQCFastQC Report
Fri 10 Feb 2017
ERR1632393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388395
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7050.1815162399104005No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5660.14572793161600947No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5370.13826130614451781No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5190.1336268489553161No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4980.12821998223458078No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4500.11586142973004288No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4120.10607757566395036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT200.001840435837.08
GGTATCA3550.032.8309861
ATACGGC702.055458E-1029.07142829
CATCGTA405.9301598E-527.759
TAAGGTA405.9301598E-527.755
TCGCCAT604.307367E-827.74999813
GCGGTAA2500.027.38000123
TACGGCT754.6020432E-1027.13333330
ACTAGAC358.8587805E-426.428573
ACGTACT358.8587805E-426.4285725
CGTGCCA2850.024.66666610
CGCGGTA3000.024.05000122
GTCGCCA701.9161052E-723.78571312
GGTAATA3000.023.43333425
AACTCCG3000.023.4333345
CGGTAAT3050.023.04918124
CTAACTC3150.022.9047623
TAACTCC3300.022.4242424
TCTTACC1005.311449E-1022.22
TATTAGA753.7275822E-722.22
Produced by FastQC (version 0.11.5)