FastQCFastQC Report
Fri 10 Feb 2017
ERR1632392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9235566
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239210.2590095723423989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227570.24640612172551202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188740.2043621365490756No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA157940.17101279986521672No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA148180.16044495811085102No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA123480.13370052252347067No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA123070.13325658654813358No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA108670.11766468887775801No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA105750.11450299851682073No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA104740.11340940013855134No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG102460.11094068300740853No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC96860.10487716724670693No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT94310.10211610203424457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147600.028.3892291
CTTATAC176150.019.21941637
ATACACA33450.018.74887837
GTATCAA228050.018.2444652
GCGGTAA63800.017.65909223
ATACGGC10450.017.17224929
GCAGTCG22800.016.7960539
TATACAC55050.016.5340637
CAGTCGG23100.016.49783510
CGTGCCA74300.015.91049810
AGTCGGT24250.015.79175211
CGCGGTA71950.015.78735322
CGGTAAT71400.015.72759124
AGCCGCG73200.015.64412619
CTCTATG33600.015.3616071
TAATACG69000.015.12173827
GGTAATA75900.015.03886725
TTCTGCG57650.014.79358218
TCTATGG34400.014.7892442
TCTTATA288300.014.64342837
Produced by FastQC (version 0.11.5)