FastQCFastQC Report
Fri 10 Feb 2017
ERR1632391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4179
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA90.21536252692031585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.21536252692031585No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC80.191433357262503No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA80.191433357262503No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.191433357262503No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC70.16750418760469013No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT70.16750418760469013TruSeq Adapter, Index 1 (95% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG70.16750418760469013No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA70.16750418760469013No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG60.14357501794687724No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT60.14357501794687724No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.14357501794687724No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.14357501794687724No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC60.14357501794687724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60.14357501794687724No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.11964584828906438No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA50.11964584828906438No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA50.11964584828906438No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC50.11964584828906438No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA50.11964584828906438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11964584828906438No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG50.11964584828906438No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.11964584828906438No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC50.11964584828906438RNA PCR Primer, Index 1 (95% over 23bp)
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG50.11964584828906438No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG50.11964584828906438No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC50.11964584828906438No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT50.11964584828906438No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)