##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4179
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.623354869586024	34.0	31.0	34.0	31.0	34.0
2	32.696099545345774	34.0	31.0	34.0	31.0	34.0
3	32.79971284996411	34.0	31.0	34.0	31.0	34.0
4	36.24383823881311	37.0	37.0	37.0	35.0	37.0
5	36.22421631969371	37.0	35.0	37.0	35.0	37.0
6	36.25412778176597	37.0	37.0	37.0	35.0	37.0
7	36.20866235941613	37.0	37.0	37.0	35.0	37.0
8	36.13137114142139	37.0	37.0	37.0	35.0	37.0
9	37.87054319215123	39.0	38.0	39.0	35.0	39.0
10	37.88202919358698	39.0	38.0	39.0	35.0	39.0
11	38.01124670973917	39.0	38.0	39.0	35.0	39.0
12	38.03541517109356	39.0	38.0	39.0	35.0	39.0
13	37.99258195740608	39.0	38.0	39.0	35.0	39.0
14	39.38358458961474	41.0	39.0	41.0	36.0	41.0
15	39.42593921990907	41.0	39.0	41.0	37.0	41.0
16	39.35702321129457	41.0	39.0	41.0	36.0	41.0
17	39.41708542713568	41.0	39.0	41.0	36.0	41.0
18	39.39650634122996	41.0	39.0	41.0	36.0	41.0
19	39.37329504666188	41.0	39.0	41.0	36.0	41.0
20	39.321368748504426	41.0	39.0	41.0	36.0	41.0
21	39.321368748504426	41.0	39.0	41.0	36.0	41.0
22	39.29289303661163	41.0	39.0	41.0	36.0	41.0
23	39.25699928212491	41.0	39.0	41.0	36.0	41.0
24	39.22517348648002	41.0	39.0	41.0	36.0	41.0
25	39.109356305336206	41.0	39.0	41.0	35.0	41.0
26	38.99736779133764	40.0	39.0	41.0	35.0	41.0
27	38.912419239052404	40.0	39.0	41.0	35.0	41.0
28	38.91983728164632	40.0	39.0	41.0	35.0	41.0
29	38.84015314668581	40.0	39.0	41.0	35.0	41.0
30	38.78152668102417	40.0	38.0	41.0	35.0	41.0
31	38.72744675759751	40.0	38.0	41.0	35.0	41.0
32	38.726011007418045	40.0	38.0	41.0	35.0	41.0
33	38.6903565446279	40.0	38.0	41.0	35.0	41.0
34	38.57310361330462	40.0	38.0	41.0	35.0	41.0
35	38.45010768126346	40.0	38.0	41.0	34.0	41.0
36	38.43670734625508	40.0	38.0	41.0	34.0	41.0
37	38.37497008853793	40.0	38.0	41.0	34.0	41.0
38	38.19047619047619	40.0	38.0	41.0	34.0	41.0
39	38.13113184972482	40.0	37.0	41.0	34.0	41.0
40	38.04666188083274	40.0	37.0	41.0	33.0	41.0
41	37.981095955970325	40.0	37.0	41.0	33.0	41.0
42	37.955731036133045	40.0	37.0	41.0	33.0	41.0
43	37.17755443886097	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	3.0
24	4.0
25	8.0
26	9.0
27	10.0
28	31.0
29	32.0
30	42.0
31	54.0
32	55.0
33	95.0
34	110.0
35	168.0
36	212.0
37	401.0
38	758.0
39	2185.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.593921990906914	16.630772912179946	13.304618329743958	27.470686767169177
2	21.12945680784877	20.076573342905	32.85474994017707	25.939219909069156
3	21.32089016511127	19.23905240488155	29.81574539363484	29.624312036372334
4	15.697535295525245	15.721464465183058	33.07011246709739	35.5108877721943
5	16.678631251495574	33.74012921751615	32.40009571667863	17.181143814309642
6	34.96051687006461	33.285474994017704	14.118210098109596	17.635798037808087
7	30.725053840631727	27.3271117492223	20.268006700167504	21.679827709978465
8	27.44675759751137	31.538645608997367	19.47834410145968	21.53625269203159
9	28.57142857142857	13.137114142139266	17.49222301986121	40.79923426657095
10	19.74156496769562	23.618090452261306	29.52859535774109	27.111749222301984
11	39.00454654223498	19.358698253170616	18.281885618569035	23.354869586025366
12	22.541277817659726	24.04881550610194	26.034936587700408	27.374970088537925
13	33.979420914094284	18.664752333094043	20.38765254845657	26.968174204355112
14	24.98205312275664	19.909069155300312	23.953098827470686	31.155778894472363
15	29.026082794927017	24.216319693706627	19.861210815984684	26.89638669538167
16	26.106724096673844	22.708782005264418	22.660923665948793	28.523570232112945
17	27.016032543670736	24.264178033022255	22.110552763819097	26.609236659487916
18	25.41277817659727	22.90021536252692	24.455611390284755	27.231395070591052
19	28.66714525005982	23.713807130892558	23.13950705910505	24.47954055994257
20	29.050011964584826	20.842306771955013	24.216319693706627	25.891361569753528
21	27.99712849964106	22.325915290739413	22.42163196937066	27.255324240248864
22	29.26537449150515	21.871261067240965	20.985881789901892	27.877482651351997
23	27.70997846374731	21.631969370662837	24.288107202680067	26.369944962909784
24	27.039961713328548	23.426657094998802	22.613065326633166	26.920315865039484
25	27.80569514237856	21.919119406556593	23.64201962191912	26.633165829145728
26	27.829624312036373	22.87628619286911	23.929169657812874	25.364919837281647
27	26.89638669538167	23.187365398420674	24.47954055994257	25.436707346255083
28	27.63819095477387	21.033740129217517	23.21129456807849	28.11677434793013
29	27.829624312036373	23.665948791576934	23.091648719789422	25.41277817659727
30	25.364919837281647	22.660923665948793	25.4606365159129	26.513519980856664
31	27.207465900933236	21.79947355826753	23.450586264656618	27.54247427614262
32	25.843503230437904	22.06269442450347	23.833452979181622	28.260349365877
33	25.19741564967696	22.373773630055037	24.55132806891601	27.877482651351997
34	25.987078248384783	22.325915290739413	23.929169657812874	27.757836803062936
35	25.173486480019143	23.498444603972242	25.436707346255083	25.891361569753528
36	28.068916008614504	22.541277817659726	23.761665470208186	25.628140703517587
37	26.106724096673844	21.12945680784877	25.532424024886335	27.231395070591052
38	25.436707346255083	21.871261067240965	26.082794927016035	26.609236659487916
39	24.575257238573823	21.608040201005025	26.346015793251976	27.470686767169177
40	24.14453218473319	21.943048576214405	26.944245034697296	26.968174204355112
41	24.168461354391003	20.291935869825316	28.882507776980137	26.657094998803544
42	22.134481933476906	20.866235941612825	28.523570232112945	28.475711892797317
43	22.684852835606602	21.368748504426897	27.85355348169419	28.092845178272313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	0.5
9	0.0
10	0.5
11	1.0
12	1.0
13	4.5
14	8.0
15	10.5
16	13.0
17	11.0
18	9.0
19	9.0
20	11.0
21	13.0
22	12.5
23	12.0
24	10.5
25	9.0
26	9.0
27	13.5
28	18.0
29	26.0
30	34.0
31	37.5
32	41.0
33	41.0
34	48.0
35	55.0
36	62.5
37	70.0
38	89.0
39	108.0
40	108.0
41	123.5
42	139.0
43	164.5
44	190.0
45	224.5
46	259.0
47	259.0
48	327.0
49	395.0
50	409.5
51	424.0
52	442.5
53	461.0
54	461.0
55	440.0
56	419.0
57	404.0
58	389.0
59	345.5
60	302.0
61	302.0
62	277.5
63	253.0
64	212.5
65	172.0
66	147.0
67	122.0
68	122.0
69	105.0
70	88.0
71	77.0
72	66.0
73	56.5
74	47.0
75	47.0
76	36.0
77	25.0
78	20.5
79	16.0
80	11.0
81	6.0
82	6.0
83	5.0
84	4.0
85	4.0
86	4.0
87	3.5
88	3.0
89	3.0
90	1.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4179.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.13615697535295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.96644295302013	82.86671452500597
2	4.590604026845638	8.183776022972003
3	1.3154362416107381	3.5175879396984926
4	0.37583892617449666	1.340033500837521
5	0.348993288590604	1.555396027757837
6	0.1610738255033557	0.8614501076812634
7	0.10738255033557045	0.6700167504187605
8	0.08053691275167785	0.574300071787509
9	0.05369127516778523	0.4307250538406317
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	9	0.21536252692031585	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.21536252692031585	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	8	0.191433357262503	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	8	0.191433357262503	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	8	0.191433357262503	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	7	0.16750418760469013	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	7	0.16750418760469013	TruSeq Adapter, Index 1 (95% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	7	0.16750418760469013	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	7	0.16750418760469013	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	6	0.14357501794687724	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	6	0.14357501794687724	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	6	0.14357501794687724	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.14357501794687724	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	6	0.14357501794687724	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14357501794687724	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.11964584828906438	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	5	0.11964584828906438	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	5	0.11964584828906438	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	5	0.11964584828906438	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	5	0.11964584828906438	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.11964584828906438	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	5	0.11964584828906438	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	5	0.11964584828906438	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	5	0.11964584828906438	RNA PCR Primer, Index 1 (95% over 23bp)
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	5	0.11964584828906438	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	5	0.11964584828906438	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	5	0.11964584828906438	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	5	0.11964584828906438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.07178750897343862	0.0
28	0.0	0.0	0.0	0.191433357262503	0.0
29	0.0	0.0	0.0	0.3589375448671931	0.0
30	0.0	0.0	0.0	0.43072505384063176	0.0
31	0.0	0.0	0.0	0.9332376166547021	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE