FastQCFastQC Report
Fri 10 Feb 2017
ERR1632388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28821
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT1120.3886055306894278TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1080.374726761736234No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA560.1943027653447139No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA540.187363380868117No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA510.1769543041532216No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC500.17348461191492315No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT480.16654522743832623No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT460.1596058429617293No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG460.1596058429617293No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA430.14919676624683392No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA430.14919676624683392No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT420.14572707400853543No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT400.1387876895319385No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT390.13531799729364005No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC370.12837861281704313No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA360.12490892057874467No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA340.11796953610214773No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA340.11796953610214773No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC340.11796953610214773No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA340.11796953610214773No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC310.10756045938725237No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC310.10756045938725237No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC300.10409076714895389No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC290.10062107491065543No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA290.10062107491065543No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA290.10062107491065543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA400.001886094623.1251
TCAACGC908.700954E-820.5555553
TATCAAC908.700954E-820.5555551
ATCAACG908.700954E-820.5555552
TCTTATA656.608489E-519.92307737
CAACGCA951.539338E-719.4736844
AACGCAG1002.6414637E-718.4999985
CAGAGTA1107.1798604E-716.8181829
AGAGTAC1107.1798604E-716.81818210
GCAGAGT1107.1798604E-716.8181828
AGTACGG1151.1427492E-616.08695612
GAGTACG1151.1427492E-616.08695611
CGCAGAG1151.1427492E-616.0869567
ACGCAGA1201.7813418E-615.4166666
GTACGGG1252.7242313E-614.79999913
CTCTTAT1000.001758284312.94999936
GTCTCTT1704.822381E-611.97058937
TCTCTTA1200.00688060710.79166635
TGTCTCT2502.78289E-710.3637
CTGTCTC3251.0491149E-68.53846237
Produced by FastQC (version 0.11.5)