##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28821
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00128378612817	34.0	33.0	34.0	31.0	34.0
2	33.13393012039832	34.0	33.0	34.0	31.0	34.0
3	33.213386072655354	34.0	33.0	34.0	31.0	34.0
4	36.518857777315155	37.0	37.0	37.0	35.0	37.0
5	36.49189826862357	37.0	37.0	37.0	35.0	37.0
6	36.522396863398214	37.0	37.0	37.0	35.0	37.0
7	36.537038964643834	37.0	37.0	37.0	35.0	37.0
8	36.47722147045557	37.0	37.0	37.0	35.0	37.0
9	38.28520870198813	39.0	39.0	39.0	37.0	39.0
10	38.31858714132056	39.0	39.0	39.0	37.0	39.0
11	38.38766871378509	39.0	39.0	39.0	37.0	39.0
12	38.347350889976056	39.0	39.0	39.0	37.0	39.0
13	38.390132195274276	39.0	39.0	39.0	37.0	39.0
14	39.9288366121925	41.0	40.0	41.0	38.0	41.0
15	39.92161965233684	41.0	40.0	41.0	38.0	41.0
16	39.88706151764338	41.0	40.0	41.0	38.0	41.0
17	39.88706151764338	41.0	40.0	41.0	38.0	41.0
18	39.89459074980049	41.0	40.0	41.0	38.0	41.0
19	39.88820651608202	41.0	40.0	41.0	38.0	41.0
20	39.869504874917595	41.0	40.0	41.0	38.0	41.0
21	39.82530099580167	41.0	40.0	41.0	38.0	41.0
22	39.80528087158669	41.0	40.0	41.0	38.0	41.0
23	39.77388015683009	41.0	40.0	41.0	38.0	41.0
24	39.73075188230804	41.0	40.0	41.0	38.0	41.0
25	39.67894937719024	41.0	40.0	41.0	37.0	41.0
26	39.588043440546826	41.0	40.0	41.0	37.0	41.0
27	39.50282779917421	41.0	40.0	41.0	37.0	41.0
28	39.4659449706811	41.0	39.0	41.0	37.0	41.0
29	39.43933243121335	41.0	39.0	41.0	37.0	41.0
30	39.39891051663717	41.0	39.0	41.0	36.0	41.0
31	39.31910759515631	41.0	39.0	41.0	36.0	41.0
32	39.28888657576073	41.0	39.0	41.0	36.0	41.0
33	39.20259532979425	41.0	39.0	41.0	35.0	41.0
34	39.172374310398666	41.0	39.0	41.0	35.0	41.0
35	39.107907428611085	41.0	39.0	41.0	35.0	41.0
36	39.0467020575275	41.0	39.0	41.0	35.0	41.0
37	38.99861212310468	41.0	39.0	41.0	35.0	41.0
38	38.9188438985462	41.0	39.0	41.0	35.0	41.0
39	38.8158634329135	40.0	38.0	41.0	35.0	41.0
40	38.72686582700115	40.0	38.0	41.0	35.0	41.0
41	38.66840151278581	40.0	38.0	41.0	35.0	41.0
42	38.57388709621456	40.0	38.0	41.0	35.0	41.0
43	37.87956698240866	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	1.0
20	2.0
21	3.0
22	5.0
23	18.0
24	21.0
25	31.0
26	40.0
27	47.0
28	74.0
29	89.0
30	146.0
31	188.0
32	228.0
33	330.0
34	439.0
35	731.0
36	1155.0
37	2105.0
38	5656.0
39	17510.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.37462267096909	16.019569064224005	12.785815898129835	27.819992366677077
2	22.10193955796121	19.68703376010548	30.90107907428611	27.3099476076472
3	21.55025849207175	19.874397140973596	28.729051733111273	29.84629263384338
4	16.27632628985809	15.048055237500435	32.899621803546026	35.77599666909545
5	17.3831581138753	33.10780333784393	32.47978904271191	17.029249505568856
6	35.03695222233788	32.986364109503484	15.131327851219597	16.845355816939037
7	30.22448908781791	27.33423545331529	19.593352069671422	22.84792338919538
8	28.2120675896048	29.783838173554006	20.266472363901322	21.73762187293987
9	27.507720065230213	12.59498282502342	17.40397626730509	42.49332084244127
10	19.496200686999064	22.95548384858263	29.232157107664552	28.316158356753757
11	39.54755213212588	18.97921654349259	18.472641476701014	23.000589847680512
12	23.149786613927343	23.604316297144443	25.741646715936294	27.50425037299192
13	35.86967835952951	17.1541584261476	19.943790985739565	27.032372228583323
14	25.002602269178727	19.600291454148017	22.330939245688906	33.06616703098435
15	29.714444328788037	24.24620936122966	19.16657992436071	26.872766385621595
16	26.380070087783213	23.718816141008293	21.81048540994414	28.090628361264358
17	27.160750841400365	24.12477013288921	21.099198501092953	27.615280524617464
18	26.782554387425833	21.022865271850385	22.969362617535825	29.225217723187953
19	28.55556712119635	22.254606016446342	22.61545400922938	26.574372853127926
20	30.509003851358386	20.662017279067346	22.129697095867595	26.699281773706673
21	27.92755282606433	21.616182644599423	21.650879566982407	28.80538496235384
22	28.878248499358104	22.244196939731445	21.10960757780785	27.767946983102597
23	28.933763575170886	21.59883418340793	21.418410187016413	28.048992054404774
24	28.139204052600537	21.977030637382462	22.129697095867595	27.754068214149406
25	28.48270358419208	22.036015405433538	22.011727559765447	27.46955345060893
26	28.718642656396376	22.670969085042156	21.723743103986674	26.88664515457479
27	27.22667499392804	22.3448180146421	23.302453072412476	27.126053919017384
28	26.76520592623434	22.969362617535825	21.612712952361125	28.65271850386871
29	27.3134172998855	23.420422608514624	21.37330418791853	27.892855903681347
30	27.008084382915236	21.942333714999478	23.74310398667638	27.306477915408905
31	27.78876513653239	22.68484785399535	21.942333714999478	27.58405329447278
32	26.286388397349153	22.157454633773984	22.372575552548486	29.18358141632837
33	26.442524548072587	21.6682280281739	24.339891051663717	27.549356372089797
34	26.720099927136467	21.525970646403664	23.510634606710383	28.243294819749487
35	26.643766697893895	22.66402970056556	24.530724124770135	26.16147947677041
36	26.879705770098195	21.57801602997814	24.544602893723326	26.997675306200343
37	26.97685715277055	21.643940182505812	23.99986121231047	27.37934145241317
38	26.32455501197044	21.17553173033552	24.912390270982964	27.58752298671108
39	25.83879809860865	20.256063287186425	26.057388709621453	27.847749904583463
40	24.98872350022553	20.578744665348182	27.636098678047254	26.79643315637903
41	23.58696783595295	19.936851601262966	28.402900662711218	28.073279900072862
42	22.112348634676103	20.870198813365253	29.43686894972416	27.580583602234483
43	21.650879566982407	21.324728496582352	28.87477880711981	28.149613129315433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	2.0
4	4.0
5	4.0
6	2.5
7	1.0
8	2.0
9	3.0
10	3.5
11	4.0
12	4.0
13	7.0
14	10.0
15	19.0
16	28.0
17	32.5
18	37.0
19	37.0
20	38.0
21	39.0
22	37.0
23	35.0
24	43.5
25	52.0
26	52.0
27	67.5
28	83.0
29	112.5
30	142.0
31	176.0
32	210.0
33	210.0
34	250.5
35	291.0
36	345.0
37	399.0
38	500.5
39	602.0
40	602.0
41	731.5
42	861.0
43	1035.5
44	1210.0
45	1545.5
46	1881.0
47	1881.0
48	2113.5
49	2346.0
50	2673.0
51	3000.0
52	3172.5
53	3345.0
54	3345.0
55	3166.5
56	2988.0
57	2835.0
58	2682.0
59	2537.5
60	2393.0
61	2393.0
62	2141.5
63	1890.0
64	1606.5
65	1323.0
66	1179.5
67	1036.0
68	1036.0
69	870.0
70	704.0
71	597.5
72	491.0
73	395.0
74	299.0
75	299.0
76	232.5
77	166.0
78	137.0
79	108.0
80	92.0
81	76.0
82	76.0
83	55.0
84	34.0
85	29.5
86	25.0
87	18.5
88	12.0
89	12.0
90	8.5
91	5.0
92	3.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	28821.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.6025814510253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82289162306132	65.37594115401963
2	5.873756658652713	8.646473057839769
3	2.045915240654316	4.517539294264599
4	0.9711026257483619	2.8590264043579334
5	0.5562626691179936	2.0471184205960933
6	0.3724131428840805	1.6446341209534716
7	0.19327770706642153	0.9958016723916588
8	0.20270588789892988	1.1935741299746714
9	0.16499316456889646	1.0929530550640159
>10	0.7731108282656861	10.304985947746435
>50	0.01414227124876255	0.5586204503660526
>100	0.009428180832508367	0.7633322924256619
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	112	0.3886055306894278	TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	108	0.374726761736234	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	56	0.1943027653447139	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	54	0.187363380868117	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	51	0.1769543041532216	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	50	0.17348461191492315	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	48	0.16654522743832623	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	46	0.1596058429617293	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	46	0.1596058429617293	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	43	0.14919676624683392	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	43	0.14919676624683392	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	42	0.14572707400853543	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	40	0.1387876895319385	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	39	0.13531799729364005	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	37	0.12837861281704313	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	36	0.12490892057874467	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	34	0.11796953610214773	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	34	0.11796953610214773	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	34	0.11796953610214773	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	34	0.11796953610214773	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	31	0.10756045938725237	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	31	0.10756045938725237	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	30	0.10409076714895389	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	29	0.10062107491065543	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	29	0.10062107491065543	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	29	0.10062107491065543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.003469692238298463	0.0
21	0.0	0.0	0.0	0.006939384476596926	0.0
22	0.0	0.0	0.0	0.01040907671489539	0.0
23	0.0	0.0	0.0	0.01040907671489539	0.0
24	0.0	0.0	0.0	0.013878768953193852	0.0
25	0.0	0.0	0.0	0.013878768953193852	0.0
26	0.0	0.0	0.0	0.02081815342979078	0.0
27	0.0	0.0	0.0	0.052045383574476946	0.0
28	0.0	0.0	0.0	0.1318483050553416	0.0
29	0.0	0.0	0.0	0.29145414801707087	0.0
30	0.0	0.0	0.0	0.4996356823149787	0.0
31	0.0	0.0	0.0	0.9992713646299574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	40	0.0018860946	23.125	1
TCAACGC	90	8.700954E-8	20.555555	3
TATCAAC	90	8.700954E-8	20.555555	1
ATCAACG	90	8.700954E-8	20.555555	2
TCTTATA	65	6.608489E-5	19.923077	37
CAACGCA	95	1.539338E-7	19.473684	4
AACGCAG	100	2.6414637E-7	18.499998	5
CAGAGTA	110	7.1798604E-7	16.818182	9
AGAGTAC	110	7.1798604E-7	16.818182	10
GCAGAGT	110	7.1798604E-7	16.818182	8
AGTACGG	115	1.1427492E-6	16.086956	12
GAGTACG	115	1.1427492E-6	16.086956	11
CGCAGAG	115	1.1427492E-6	16.086956	7
ACGCAGA	120	1.7813418E-6	15.416666	6
GTACGGG	125	2.7242313E-6	14.799999	13
CTCTTAT	100	0.0017582843	12.949999	36
GTCTCTT	170	4.822381E-6	11.970589	37
TCTCTTA	120	0.006880607	10.791666	35
TGTCTCT	250	2.78289E-7	10.36	37
CTGTCTC	325	1.0491149E-6	8.538462	37
>>END_MODULE