FastQCFastQC Report
Fri 10 Feb 2017
ERR1632387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26695
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1480.5544109383779734No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1130.4233002434912905No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA810.3034276081663233No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC790.2959355684585129No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC780.2921895486046076No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT770.2884435287507024No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA720.26971342948117627No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT680.25472935006555536No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT670.2509833302116501No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA670.2509833302116501No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA670.2509833302116501No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC660.2472373103577449No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA600.22476119123431357No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT560.20977711181869263Illumina PCR Primer Index 10 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT550.2060310919647874No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA550.2060310919647874No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA540.2022850721108822No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC530.19853905225697696No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT530.19853905225697696No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC520.19479303240307175No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA520.19479303240307175No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA480.17980895298745084No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC470.17606293313354562No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA460.17231691327964038No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC450.16857089342573517No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC450.16857089342573517No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC440.16482487357182993No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA440.16482487357182993No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG440.16482487357182993No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC410.15358681401011426No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC410.15358681401011426No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG400.14984079415620902No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC400.14984079415620902No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC390.1460947743023038No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT380.1423487544483986No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA380.1423487544483986No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG380.1423487544483986No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC380.1423487544483986No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA370.13860273459449335No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA370.13860273459449335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT360.13485671474058814No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA360.13485671474058814No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC360.13485671474058814No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT360.13485671474058814No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG360.13485671474058814No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT340.12736467503277768No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA340.12736467503277768No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT330.12361865517887245No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC330.12361865517887245No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC330.12361865517887245No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT320.11987263532496721No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA320.11987263532496721No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT320.11987263532496721No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG310.116126615471062No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA310.116126615471062No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG310.116126615471062No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA300.11238059561715678No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG300.11238059561715678No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG300.11238059561715678No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC300.11238059561715678No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA300.11238059561715678No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG290.10863457576325154No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT280.10488855590934631No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT280.10488855590934631No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA280.10488855590934631No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC280.10488855590934631No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG270.1011425360554411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA705.456968E-1231.7142871
CTTATAC555.885304E-726.9090937
GTATCAA1058.62201E-1021.1428592
TCTTATA852.5653007E-517.41176436
CTAACTC751.973494E-417.2666663
TAACTCC803.230205E-416.18754
GCGGTAA700.002504521515.85714323
AACTCCG700.002504521515.8571435
GCTAACT700.002504521515.8571432
CGCGGTA700.002504521515.85714322
ACGCAGA1609.451469E-915.031258
AACGCAG1507.1355316E-814.87
CCGTGCC750.00396626114.89
CCAGCAG750.00396626114.814
TACGGGC750.00396626114.814
TCAACGC1507.1355316E-814.85
GCAGCCG750.00396626114.817
GGCTAAC750.00396626114.81
TCCGTGC750.00396626114.88
ATCAACG1507.1355316E-814.84
Produced by FastQC (version 0.11.5)