##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632387.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26695
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.031429106574265	34.0	33.0	34.0	31.0	34.0
2	33.151189361303615	34.0	33.0	34.0	31.0	34.0
3	33.225585315602174	34.0	33.0	34.0	31.0	34.0
4	36.51219329462446	37.0	37.0	37.0	35.0	37.0
5	36.49144034463383	37.0	37.0	37.0	35.0	37.0
6	36.54751826184679	37.0	37.0	37.0	35.0	37.0
7	36.540176062933135	37.0	37.0	37.0	35.0	37.0
8	36.5161640756696	37.0	37.0	37.0	35.0	37.0
9	38.30103015545983	39.0	39.0	39.0	37.0	39.0
10	38.35665855029032	39.0	39.0	39.0	37.0	39.0
11	38.422700880314665	39.0	39.0	39.0	37.0	39.0
12	38.36587375913092	39.0	39.0	39.0	37.0	39.0
13	38.420228507211085	39.0	39.0	39.0	37.0	39.0
14	39.905225697696196	41.0	40.0	41.0	38.0	41.0
15	39.92893800337142	41.0	40.0	41.0	38.0	41.0
16	39.865780108634574	41.0	40.0	41.0	38.0	41.0
17	39.90237872260723	41.0	40.0	41.0	38.0	41.0
18	39.930136729724666	41.0	40.0	41.0	38.0	41.0
19	39.8995692077168	41.0	40.0	41.0	38.0	41.0
20	39.911968533433225	41.0	40.0	41.0	38.0	41.0
21	39.87083723543735	41.0	40.0	41.0	38.0	41.0
22	39.82749578572766	41.0	40.0	41.0	38.0	41.0
23	39.79610413935194	41.0	40.0	41.0	38.0	41.0
24	39.76227758007118	41.0	40.0	41.0	38.0	41.0
25	39.73556845851283	41.0	40.0	41.0	38.0	41.0
26	39.60955235062746	41.0	40.0	41.0	37.0	41.0
27	39.486682899419364	41.0	39.0	41.0	37.0	41.0
28	39.43746019853905	41.0	39.0	41.0	37.0	41.0
29	39.40071174377224	41.0	39.0	41.0	36.0	41.0
30	39.376812137104324	41.0	39.0	41.0	36.0	41.0
31	39.27102453643004	41.0	39.0	41.0	36.0	41.0
32	39.22449896984454	41.0	39.0	41.0	35.0	41.0
33	39.13549353811575	41.0	39.0	41.0	35.0	41.0
34	39.09945682712118	41.0	39.0	41.0	35.0	41.0
35	39.02573515639633	41.0	39.0	41.0	35.0	41.0
36	38.930136729724666	40.0	39.0	41.0	35.0	41.0
37	38.86885184491478	40.0	39.0	41.0	35.0	41.0
38	38.81887994006368	40.0	38.0	41.0	35.0	41.0
39	38.700206031091966	40.0	38.0	41.0	35.0	41.0
40	38.594755572204534	40.0	38.0	41.0	35.0	41.0
41	38.56328900543173	40.0	38.0	41.0	35.0	41.0
42	38.45581569582319	40.0	38.0	41.0	35.0	41.0
43	37.77370294062558	40.0	36.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	5.0
22	6.0
23	14.0
24	26.0
25	16.0
26	40.0
27	57.0
28	57.0
29	93.0
30	121.0
31	159.0
32	194.0
33	290.0
34	412.0
35	728.0
36	1121.0
37	2233.0
38	5196.0
39	15924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19535493538116	15.564712492976213	12.477992133358306	28.761940438284324
2	22.670912155834426	19.64787413373291	30.41018917400262	27.271024536430044
3	20.797902228881814	18.86120996441281	29.683461322345007	30.657426484360368
4	14.894174939127177	15.976774676905789	36.59486795280015	32.53418243116688
5	16.587375913092337	33.219704064431546	33.433227196104134	16.75969282637198
6	31.264281700693015	35.815695823187866	16.400074920397078	16.519947555722045
7	28.589623525004683	27.810451395392395	20.10863457576325	23.49129050383967
8	26.705375538490355	29.578572766435663	21.307360929012926	22.40869076606106
9	27.563214085034648	12.155834425922457	17.14178685147031	43.13916463757258
10	20.73796591121933	21.6482487357183	27.050009365049632	30.563775988012736
11	41.086345757632515	18.490353998876195	17.565087094961605	22.85821314852969
12	22.521071361678217	23.99325716426297	26.07979022288818	27.405881251170634
13	37.85727664356621	16.804645064618843	19.314478366735344	26.023599925079605
14	23.97078104513954	19.96628582131485	23.21033901479678	32.852594118748826
15	30.86720359617906	22.757070612474244	19.82768308672036	26.548042704626333
16	24.00824124367859	24.300430792283198	22.906911406630456	28.784416557407756
17	28.01273646750328	23.49878254354748	20.340887806705375	28.147593182243867
18	27.926578010863455	18.992320659299494	22.232627832927516	30.848473496909534
19	29.069114066304554	21.67821689454954	22.378722607229818	26.87394643191609
20	32.49672223262783	19.24705000936505	20.962727102453645	27.293500655553476
21	28.949241430979583	20.490728600861587	20.453268402322532	30.106761565836297
22	29.578572766435663	22.067802959355685	19.269526128488483	29.084098145720173
23	30.54504588874321	20.434538303053007	20.88780670537554	28.132609102828244
24	28.312418055815698	19.681588312418054	22.40119872635325	29.604794905413
25	29.822064056939503	21.30361490915902	21.955422363738528	26.91889867016295
26	29.586064806143476	22.5810076793407	21.243678591496533	26.589248923019294
27	27.143659861397268	20.70799775238809	24.585128301179996	27.563214085034648
28	26.88893051133171	22.584753699194607	20.681775613410753	29.844540176062935
29	28.0277205469189	23.925828806892678	20.46076044203034	27.58569020415808
30	27.45083348941749	21.228694512080914	23.348941749391273	27.971530249110323
31	28.904289192732723	22.153961415995504	22.004120621839295	26.937628769432475
32	26.188424798651432	20.9327589436224	22.168945495411126	30.709870762315038
33	26.023599925079605	20.98894924143098	24.877317849784603	28.110132983704816
34	26.49934444652557	21.187488293687956	23.910844727477055	28.40232253230942
35	25.693950177935942	23.764749953174753	24.506461884247987	26.034837984641317
36	26.4506461884248	22.210151713804084	24.877317849784603	26.461884247986518
37	27.158643940812887	22.02285072110882	24.086907660610603	26.73159767746769
38	26.027345944933504	20.95898108259974	25.68271211837423	27.330960854092528
39	24.95223824686271	19.715302491103202	26.566772803895862	28.76568645813823
40	24.697508896797153	21.344821127551977	28.0277205469189	25.929949428731973
41	23.101704439033526	19.60666791533995	28.563401386027348	28.728226259599175
42	20.32215770743585	20.696759692826372	31.050758569020413	27.93032403071736
43	19.93631766248361	21.06012361865518	29.91571455328713	29.08784416557408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.5
7	2.0
8	1.5
9	1.0
10	2.5
11	4.0
12	4.0
13	8.5
14	13.0
15	25.5
16	38.0
17	34.0
18	30.0
19	30.0
20	41.0
21	52.0
22	34.0
23	16.0
24	22.5
25	29.0
26	29.0
27	30.0
28	31.0
29	37.5
30	44.0
31	52.0
32	60.0
33	60.0
34	98.0
35	136.0
36	162.5
37	189.0
38	256.0
39	323.0
40	323.0
41	421.5
42	520.0
43	706.5
44	893.0
45	1476.5
46	2060.0
47	2060.0
48	2361.5
49	2663.0
50	2967.5
51	3272.0
52	3319.0
53	3366.0
54	3366.0
55	3138.0
56	2910.0
57	2735.0
58	2560.0
59	2380.5
60	2201.0
61	2201.0
62	2078.0
63	1955.0
64	1524.5
65	1094.0
66	952.0
67	810.0
68	810.0
69	677.5
70	545.0
71	466.0
72	387.0
73	303.0
74	219.0
75	219.0
76	165.5
77	112.0
78	95.5
79	79.0
80	57.0
81	35.0
82	35.0
83	30.0
84	25.0
85	18.5
86	12.0
87	8.5
88	5.0
89	5.0
90	2.5
91	0.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.74133732908784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.62987054397041	49.125304364113134
2	7.346470250621771	8.63082974339764
3	3.0737835597219565	5.416744708746957
4	1.6835660991008226	3.9557969657239185
5	0.8354059052356354	2.453643004307923
6	0.6823544416810152	2.404944746207155
7	0.47190867929341235	1.9404382843229069
8	0.2933486384796888	1.378535306237123
9	0.2487086282762579	1.3148529687207342
>10	1.6006632230087365	17.86102266342012
>50	0.12116574198074102	4.540176062933134
>100	0.012754288629551688	0.977711181869264
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	148	0.5544109383779734	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	113	0.4233002434912905	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	81	0.3034276081663233	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	79	0.2959355684585129	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	78	0.2921895486046076	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	77	0.2884435287507024	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	72	0.26971342948117627	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	68	0.25472935006555536	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	67	0.2509833302116501	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	67	0.2509833302116501	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	67	0.2509833302116501	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	66	0.2472373103577449	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	60	0.22476119123431357	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	56	0.20977711181869263	Illumina PCR Primer Index 10 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	55	0.2060310919647874	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	55	0.2060310919647874	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	54	0.2022850721108822	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	53	0.19853905225697696	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	53	0.19853905225697696	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	52	0.19479303240307175	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	52	0.19479303240307175	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	48	0.17980895298745084	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	47	0.17606293313354562	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	46	0.17231691327964038	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	45	0.16857089342573517	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	45	0.16857089342573517	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	44	0.16482487357182993	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	44	0.16482487357182993	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	44	0.16482487357182993	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	41	0.15358681401011426	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	41	0.15358681401011426	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	40	0.14984079415620902	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	40	0.14984079415620902	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	39	0.1460947743023038	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	38	0.1423487544483986	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	38	0.1423487544483986	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	38	0.1423487544483986	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	38	0.1423487544483986	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	37	0.13860273459449335	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	37	0.13860273459449335	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.13485671474058814	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	36	0.13485671474058814	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	36	0.13485671474058814	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	36	0.13485671474058814	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	36	0.13485671474058814	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	34	0.12736467503277768	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	34	0.12736467503277768	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	33	0.12361865517887245	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	33	0.12361865517887245	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	33	0.12361865517887245	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	32	0.11987263532496721	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	32	0.11987263532496721	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	32	0.11987263532496721	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	31	0.116126615471062	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	31	0.116126615471062	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	31	0.116126615471062	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	30	0.11238059561715678	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	30	0.11238059561715678	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	30	0.11238059561715678	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	30	0.11238059561715678	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	30	0.11238059561715678	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	29	0.10863457576325154	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	28	0.10488855590934631	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	28	0.10488855590934631	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	28	0.10488855590934631	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	28	0.10488855590934631	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	27	0.1011425360554411	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0037460198539052258	0.0
20	0.0	0.0	0.0	0.0037460198539052258	0.0
21	0.0	0.0	0.0	0.0074920397078104516	0.0
22	0.0	0.0	0.0	0.022476119123431355	0.0
23	0.0	0.0	0.0	0.022476119123431355	0.0
24	0.0	0.0	0.0	0.022476119123431355	0.0
25	0.0	0.0	0.0	0.022476119123431355	0.0
26	0.0	0.0	0.0	0.02622213897733658	0.0
27	0.0	0.0	0.0	0.056190297808578385	0.0
28	0.0	0.0	0.0	0.19479303240307175	0.0
29	0.0	0.0	0.0	0.40457014422176435	0.0
30	0.0	0.0	0.0	0.7192358119498033	0.0
31	0.0	0.0	0.0	1.4010114253605543	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	70	5.456968E-12	31.714287	1
CTTATAC	55	5.885304E-7	26.90909	37
GTATCAA	105	8.62201E-10	21.142859	2
TCTTATA	85	2.5653007E-5	17.411764	36
CTAACTC	75	1.973494E-4	17.266666	3
TAACTCC	80	3.230205E-4	16.1875	4
GCGGTAA	70	0.0025045215	15.857143	23
AACTCCG	70	0.0025045215	15.857143	5
GCTAACT	70	0.0025045215	15.857143	2
CGCGGTA	70	0.0025045215	15.857143	22
ACGCAGA	160	9.451469E-9	15.03125	8
AACGCAG	150	7.1355316E-8	14.8	7
CCGTGCC	75	0.003966261	14.8	9
CCAGCAG	75	0.003966261	14.8	14
TACGGGC	75	0.003966261	14.8	14
TCAACGC	150	7.1355316E-8	14.8	5
GCAGCCG	75	0.003966261	14.8	17
GGCTAAC	75	0.003966261	14.8	1
TCCGTGC	75	0.003966261	14.8	8
ATCAACG	150	7.1355316E-8	14.8	4
>>END_MODULE