FastQCFastQC Report
Fri 10 Feb 2017
ERR1632386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA110.290084388185654No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC100.26371308016877637RNA PCR Primer, Index 3 (95% over 22bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.23734177215189875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90.23734177215189875No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA80.21097046413502107No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.21097046413502107No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC70.18459915611814345No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA70.18459915611814345No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT70.18459915611814345No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA60.15822784810126583No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA60.15822784810126583No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA60.15822784810126583No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT60.15822784810126583TruSeq Adapter, Index 3 (95% over 21bp)
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG60.15822784810126583No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA60.15822784810126583No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC50.13185654008438819No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG50.13185654008438819No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC50.13185654008438819No Hit
TAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCAT50.13185654008438819No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC50.13185654008438819No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA50.13185654008438819No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG50.13185654008438819No Hit
CATCTGGCCGGTCTCGTCCACGGAGAACTCCGCGACGATGTTG50.13185654008438819No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA50.13185654008438819No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA50.13185654008438819No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC50.13185654008438819No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA50.13185654008438819No Hit
GGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGT40.10548523206751054No Hit
TTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGG40.10548523206751054No Hit
GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATC40.10548523206751054No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.10548523206751054No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC40.10548523206751054No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG40.10548523206751054No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG40.10548523206751054No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC40.10548523206751054No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT40.10548523206751054No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC40.10548523206751054No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA40.10548523206751054No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC40.10548523206751054No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC40.10548523206751054No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA40.10548523206751054No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT40.10548523206751054No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGC40.10548523206751054No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)