##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3792
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.796149789029535	34.0	31.0	34.0	31.0	34.0
2	32.96228902953587	34.0	33.0	34.0	31.0	34.0
3	33.01292194092827	34.0	33.0	34.0	31.0	34.0
4	36.360759493670884	37.0	37.0	37.0	35.0	37.0
5	36.3125	37.0	37.0	37.0	35.0	37.0
6	36.32647679324894	37.0	37.0	37.0	35.0	37.0
7	36.354957805907176	37.0	37.0	37.0	35.0	37.0
8	36.30036919831224	37.0	37.0	37.0	35.0	37.0
9	38.048259493670884	39.0	39.0	39.0	37.0	39.0
10	38.12236286919831	39.0	38.0	39.0	37.0	39.0
11	38.220464135021096	39.0	39.0	39.0	37.0	39.0
12	38.123681434599156	39.0	39.0	39.0	37.0	39.0
13	38.16402953586498	39.0	39.0	39.0	37.0	39.0
14	39.64477848101266	41.0	40.0	41.0	37.0	41.0
15	39.652426160337555	41.0	40.0	41.0	37.0	41.0
16	39.58834388185654	41.0	39.0	41.0	37.0	41.0
17	39.61787974683544	41.0	40.0	41.0	37.0	41.0
18	39.586497890295355	41.0	40.0	41.0	37.0	41.0
19	39.676160337552744	41.0	40.0	41.0	37.0	41.0
20	39.57304852320675	41.0	40.0	41.0	37.0	41.0
21	39.638449367088604	41.0	40.0	41.0	37.0	41.0
22	39.47784810126582	41.0	39.0	41.0	37.0	41.0
23	39.492616033755276	41.0	39.0	41.0	37.0	41.0
24	39.512394514767934	41.0	39.0	41.0	37.0	41.0
25	39.45226793248945	41.0	39.0	41.0	36.0	41.0
26	39.36445147679325	41.0	39.0	41.0	36.0	41.0
27	39.31909282700422	41.0	39.0	41.0	36.0	41.0
28	39.28138185654009	41.0	39.0	41.0	36.0	41.0
29	39.16191983122363	41.0	39.0	41.0	35.0	41.0
30	39.13449367088607	41.0	39.0	41.0	35.0	41.0
31	39.078322784810126	41.0	39.0	41.0	35.0	41.0
32	39.04351265822785	41.0	39.0	41.0	35.0	41.0
33	39.02215189873418	40.0	39.0	41.0	35.0	41.0
34	38.94752109704641	41.0	39.0	41.0	35.0	41.0
35	38.815400843881854	40.0	38.0	41.0	35.0	41.0
36	38.7542194092827	40.0	39.0	41.0	35.0	41.0
37	38.75922995780591	40.0	38.0	41.0	35.0	41.0
38	38.60311181434599	40.0	38.0	41.0	35.0	41.0
39	38.55801687763713	40.0	38.0	41.0	35.0	41.0
40	38.48074894514768	40.0	38.0	41.0	35.0	41.0
41	38.401898734177216	40.0	38.0	41.0	35.0	41.0
42	38.285073839662445	40.0	38.0	41.0	34.0	41.0
43	37.55590717299578	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	3.0
25	6.0
26	7.0
27	7.0
28	16.0
29	18.0
30	30.0
31	35.0
32	42.0
33	58.0
34	82.0
35	134.0
36	159.0
37	335.0
38	745.0
39	2113.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.220464135021096	17.009493670886076	12.341772151898734	28.428270042194093
2	20.569620253164558	21.070675105485233	33.016877637130804	25.34282700421941
3	21.75632911392405	19.27742616033755	29.140295358649787	29.825949367088604
4	16.323839662447256	15.374472573839663	32.383966244725734	35.91772151898734
5	17.536919831223628	33.016877637130804	33.46518987341772	15.981012658227847
6	34.86286919831223	33.12236286919831	15.163502109704641	16.85126582278481
7	31.381856540084392	26.424050632911396	20.411392405063292	21.78270042194093
8	26.081223628691987	31.170886075949365	20.5168776371308	22.231012658227847
9	28.375527426160335	13.05379746835443	16.719409282700422	41.85126582278481
10	19.488396624472575	23.99789029535865	29.93143459915612	26.582278481012654
11	39.60970464135021	19.33016877637131	17.906118143459913	23.154008438818565
12	23.602320675105485	23.259493670886076	25.97573839662447	27.162447257383963
13	34.889240506329116	17.87974683544304	20.279535864978904	26.951476793248947
14	23.866033755274263	20.912447257383967	23.20675105485232	32.01476793248945
15	30.247890295358648	23.074894514767934	20.96518987341772	25.712025316455694
16	26.845991561181435	22.837552742616033	23.470464135021096	26.845991561181435
17	25.922995780590718	23.99789029535865	22.020042194092827	28.059071729957807
18	26.529535864978904	21.809071729957804	23.523206751054854	28.138185654008442
19	27.76898734177215	22.943037974683545	22.811181434599156	26.476793248945146
20	27.953586497890292	22.389240506329113	22.89029535864979	26.766877637130804
21	27.979957805907173	22.020042194092827	23.2331223628692	26.766877637130804
22	27.90084388185654	21.940928270042196	22.758438818565402	27.399789029535864
23	27.267932489451475	21.78270042194093	23.76054852320675	27.188818565400847
24	28.982067510548525	20.991561181434598	21.598101265822788	28.428270042194093
25	28.665611814345993	22.178270042194093	22.231012658227847	26.925105485232066
26	28.982067510548525	22.09915611814346	23.048523206751053	25.87025316455696
27	25.81751054852321	22.547468354430382	24.34071729957806	27.294303797468356
28	26.503164556962027	21.940928270042196	24.34071729957806	27.21518987341772
29	27.953586497890292	24.34071729957806	21.729957805907173	25.97573839662447
30	25.81751054852321	22.78481012658228	23.259493670886076	28.138185654008442
31	28.059071729957807	22.969409282700422	22.600210970464136	26.371308016877638
32	25.395569620253166	21.940928270042196	23.866033755274263	28.79746835443038
33	26.55590717299578	21.09704641350211	24.077004219409282	28.270042194092827
34	26.265822784810126	22.020042194092827	24.63080168776371	27.083333333333332
35	26.845991561181435	23.39135021097046	23.54957805907173	26.213080168776372
36	26.186708860759495	22.231012658227847	25.448312236286917	26.133966244725737
37	26.34493670886076	22.600210970464136	24.65717299578059	26.39767932489451
38	24.551687763713083	21.940928270042196	25.29008438818565	28.217299578059073
39	25.712025316455694	20.279535864978904	26.951476793248947	27.056962025316455
40	25.131856540084392	20.833333333333336	26.503164556962027	27.531645569620256
41	23.866033755274263	19.40928270042194	28.82383966244726	27.90084388185654
42	23.839662447257385	21.54535864978903	28.744725738396625	25.87025316455696
43	21.044303797468356	22.943037974683545	28.850210970464136	27.162447257383963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	2.0
10	1.0
11	0.0
12	0.0
13	2.0
14	4.0
15	7.0
16	10.0
17	7.5
18	5.0
19	5.0
20	12.0
21	19.0
22	14.5
23	10.0
24	9.0
25	8.0
26	8.0
27	11.5
28	15.0
29	20.0
30	25.0
31	27.0
32	29.0
33	29.0
34	40.5
35	52.0
36	51.5
37	51.0
38	78.0
39	105.0
40	105.0
41	115.5
42	126.0
43	148.5
44	171.0
45	222.0
46	273.0
47	273.0
48	286.5
49	300.0
50	345.0
51	390.0
52	400.5
53	411.0
54	411.0
55	380.0
56	349.0
57	352.0
58	355.0
59	334.0
60	313.0
61	313.0
62	293.0
63	273.0
64	214.5
65	156.0
66	134.0
67	112.0
68	112.0
69	94.5
70	77.0
71	68.5
72	60.0
73	51.5
74	43.0
75	43.0
76	29.0
77	15.0
78	12.5
79	10.0
80	9.5
81	9.0
82	9.0
83	5.5
84	2.0
85	4.0
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3792.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.84282700421942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.04443110177124	80.85443037974683
2	5.2836985890123085	9.282700421940929
3	1.3809666766736717	3.6392405063291138
4	0.48033623536475534	1.6877637130801686
5	0.3602521765235665	1.5822784810126582
6	0.18012608826178325	0.949367088607595
7	0.09006304413089163	0.5537974683544303
8	0.06004202942059442	0.42194092827004215
9	0.06004202942059442	0.4746835443037975
>10	0.06004202942059442	0.5537974683544303
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	11	0.290084388185654	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	10	0.26371308016877637	RNA PCR Primer, Index 3 (95% over 22bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	9	0.23734177215189875	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.23734177215189875	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	8	0.21097046413502107	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	8	0.21097046413502107	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	7	0.18459915611814345	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	7	0.18459915611814345	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	7	0.18459915611814345	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	6	0.15822784810126583	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	6	0.15822784810126583	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	6	0.15822784810126583	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	6	0.15822784810126583	TruSeq Adapter, Index 3 (95% over 21bp)
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	6	0.15822784810126583	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	6	0.15822784810126583	No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC	5	0.13185654008438819	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	5	0.13185654008438819	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	5	0.13185654008438819	No Hit
TAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCAT	5	0.13185654008438819	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	5	0.13185654008438819	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	5	0.13185654008438819	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	5	0.13185654008438819	No Hit
CATCTGGCCGGTCTCGTCCACGGAGAACTCCGCGACGATGTTG	5	0.13185654008438819	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	5	0.13185654008438819	No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA	5	0.13185654008438819	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	5	0.13185654008438819	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	5	0.13185654008438819	No Hit
GGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGT	4	0.10548523206751054	No Hit
TTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGG	4	0.10548523206751054	No Hit
GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATC	4	0.10548523206751054	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	4	0.10548523206751054	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	4	0.10548523206751054	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	4	0.10548523206751054	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	4	0.10548523206751054	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	4	0.10548523206751054	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	4	0.10548523206751054	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	4	0.10548523206751054	No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA	4	0.10548523206751054	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	4	0.10548523206751054	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	4	0.10548523206751054	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	4	0.10548523206751054	No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT	4	0.10548523206751054	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGC	4	0.10548523206751054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.026371308016877638	0.0
28	0.0	0.0	0.0	0.10548523206751055	0.0
29	0.0	0.0	0.0	0.31645569620253167	0.0
30	0.0	0.0	0.0	0.44831223628691985	0.0
31	0.0	0.0	0.0	1.0284810126582278	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE