FastQCFastQC Report
Fri 10 Feb 2017
ERR1632384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37610
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA810.2153682531241691No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA750.19941504918904546No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA640.1701675086413188No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA500.1329433661260303No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT440.11699016219090666No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA440.11699016219090666No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC430.11433129486838607No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT410.10901356022334485No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA400.10635469290082426No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG400.10635469290082426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380.10103695825578304No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT380.10103695825578304RNA PCR Primer, Index 33 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA802.910383E-1127.751
GGTAATA451.290268E-424.66666825
CTCTTAT1051.8189894E-1224.66666637
AGCCGCG400.0018968523.12519
CCGTGCC450.003758426520.5555579
GCGGTAA450.003758426520.55555723
AACTCCG450.003758426520.5555575
GCAGCCG450.003758426520.55555717
CTAACTC450.003758426520.5555573
GCTAACT450.003758426520.5555572
CGCGGTA450.003758426520.55555722
CGTGCCA450.003758426520.55555710
GTATCAA1101.6007107E-920.1818182
CTCCGTG555.017095E-420.1818187
TACGGAG500.00691216118.530
TAACTCC500.00691216118.54
ATACGGA500.00691216118.529
TAATACG500.00691216118.527
TCCGTGC500.00691216118.58
AATACGG500.00691216118.528