FastQCFastQC Report
Fri 10 Feb 2017
ERR1632383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT190.3989082511022464TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA120.2519420533277346No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT100.20995171110644553No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC100.20995171110644553No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC90.18895653999580095No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT90.18895653999580095No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA90.18895653999580095No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC80.1679613688851564No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA80.1679613688851564No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA80.1679613688851564No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA70.14696619777451186No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA70.14696619777451186No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA70.14696619777451186No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG60.1259710266638673No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC60.1259710266638673No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.1259710266638673No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC60.1259710266638673No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC60.1259710266638673No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA60.1259710266638673No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT60.1259710266638673No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG60.1259710266638673No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT50.10497585555322277No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.10497585555322277No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC50.10497585555322277Illumina Paired End PCR Primer 2 (95% over 23bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC50.10497585555322277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10497585555322277No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA50.10497585555322277No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG50.10497585555322277No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG50.10497585555322277No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC50.10497585555322277No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG50.10497585555322277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCT250.00496976329.637
Produced by FastQC (version 0.11.5)