##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632383.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4763
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92441738400168	34.0	33.0	34.0	31.0	34.0
2	33.07117363006508	34.0	33.0	34.0	31.0	34.0
3	33.138988032752465	34.0	33.0	34.0	31.0	34.0
4	36.44425782070124	37.0	37.0	37.0	35.0	37.0
5	36.42095318076842	37.0	37.0	37.0	35.0	37.0
6	36.4560151165232	37.0	37.0	37.0	35.0	37.0
7	36.45097627545665	37.0	37.0	37.0	35.0	37.0
8	36.40226747847995	37.0	37.0	37.0	35.0	37.0
9	38.22002939323956	39.0	39.0	39.0	37.0	39.0
10	38.2053327734621	39.0	39.0	39.0	37.0	39.0
11	38.28070543774932	39.0	39.0	39.0	37.0	39.0
12	38.279865630904894	39.0	39.0	39.0	37.0	39.0
13	38.26138988032753	39.0	39.0	39.0	37.0	39.0
14	39.82217090069284	41.0	40.0	41.0	38.0	41.0
15	39.770522779760654	41.0	40.0	41.0	38.0	41.0
16	39.75687591853874	41.0	40.0	41.0	38.0	41.0
17	39.70984673525089	41.0	40.0	41.0	38.0	41.0
18	39.7134159143397	41.0	40.0	41.0	37.0	41.0
19	39.745118622716774	41.0	40.0	41.0	38.0	41.0
20	39.74574847785009	41.0	40.0	41.0	38.0	41.0
21	39.71887465882847	41.0	40.0	41.0	38.0	41.0
22	39.648330883896705	41.0	40.0	41.0	37.0	41.0
23	39.657778710896494	41.0	40.0	41.0	37.0	41.0
24	39.58597522569809	41.0	40.0	41.0	37.0	41.0
25	39.564350199454125	41.0	40.0	41.0	37.0	41.0
26	39.50682343061096	41.0	39.0	41.0	37.0	41.0
27	39.344530757925675	41.0	39.0	41.0	36.0	41.0
28	39.374343900902794	41.0	39.0	41.0	36.0	41.0
29	39.306949401637624	41.0	39.0	41.0	36.0	41.0
30	39.245013646861224	41.0	39.0	41.0	36.0	41.0
31	39.19273567079572	41.0	39.0	41.0	36.0	41.0
32	39.112534117153054	41.0	39.0	41.0	35.0	41.0
33	39.02246483308839	41.0	39.0	41.0	35.0	41.0
34	38.97669536006718	41.0	39.0	41.0	35.0	41.0
35	38.91937854293513	41.0	39.0	41.0	35.0	41.0
36	38.84379592693681	40.0	39.0	41.0	35.0	41.0
37	38.789838337182445	40.0	38.0	41.0	35.0	41.0
38	38.697459584295615	40.0	38.0	41.0	35.0	41.0
39	38.63741339491917	40.0	38.0	41.0	35.0	41.0
40	38.563300440898594	40.0	38.0	41.0	35.0	41.0
41	38.50766323745538	40.0	38.0	41.0	35.0	41.0
42	38.423472601301704	40.0	38.0	41.0	35.0	41.0
43	37.68003359227378	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	4.0
24	1.0
25	5.0
26	10.0
27	8.0
28	18.0
29	26.0
30	27.0
31	40.0
32	42.0
33	75.0
34	94.0
35	138.0
36	188.0
37	357.0
38	901.0
39	2825.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.01910560571069	17.48897753516691	11.94625236195675	27.54566449716565
2	21.268108335082932	18.790678144026874	31.072853243753933	28.868360277136258
3	20.764224228427462	20.134369095108127	28.28049548603821	30.8209111904262
4	17.02708377073273	15.620407306319548	32.22758765483938	35.12492126810833
5	17.677934075162714	32.43753936594583	32.83644761704808	17.048078941843375
6	36.30065085030443	33.00440898593324	13.772832248582825	16.92210791517951
7	30.359017425992025	28.490447197144658	20.239344950661348	20.911190426201973
8	27.335712786059208	30.904891874868778	19.756456015116523	22.002939323955488
9	27.67163552382952	12.576107495276087	16.670165861851775	43.08209111904262
10	18.95863951291203	23.409615788368676	29.309258870459793	28.322485828259502
11	40.898593323535586	18.53873609069914	17.195045139617886	23.367625446147386
12	23.11568339281965	23.80852403947092	25.40415704387991	27.67163552382952
13	35.98572328364476	17.404996850724334	20.071383581776193	26.53789628385471
14	23.724543355028345	19.6514801595633	21.100146966197777	35.52382951921058
15	30.275036741549442	24.144446777241235	19.399538106235568	26.18097837497376
16	28.847365106025613	23.34663027503674	20.176359437329413	27.629645181608232
17	26.013017006088603	23.57757715725383	21.436069703968087	28.973336132689482
18	25.551123241654423	21.07915179508713	23.493596472811255	29.876128490447197
19	29.183287843795924	22.338862061725802	22.170900692840647	26.306949401637624
20	29.078311988242707	21.07915179508713	23.47260130170061	26.369934914969555
21	27.755616208272098	21.7719924417384	22.04492966617678	28.427461683812723
22	29.603191266008817	22.212891035061936	20.218349779550703	27.965567919378543
23	27.86059206382532	21.436069703968087	21.939953810623557	28.763384421583037
24	29.036321646021413	22.212891035061936	20.61725803065295	28.1335292882637
25	28.763384421583037	22.107915179508712	22.69577997060676	26.432920428301493
26	28.448456854923364	21.688011757295822	21.897963468402267	27.965567919378543
27	26.726852823850518	21.9609489817342	22.947722023934496	28.364476170480792
28	24.480369515011546	22.779760655049337	22.380852403947095	30.359017425992025
29	28.364476170480792	23.325635103926096	21.352089019525508	26.957799706067604
30	27.188746588284694	22.023934495066136	23.34663027503674	27.440688641612432
31	29.036321646021413	20.512282175099728	22.96871719504514	27.48267898383372
32	26.24396388830569	21.100146966197777	23.094688221709006	29.561200923787528
33	27.797606550493388	21.352089019525508	22.88473651060256	27.965567919378543
34	27.27272727272727	22.065924837287422	22.92672685282385	27.734621037161457
35	25.887045979424734	22.716775141717406	24.43837917279026	26.957799706067604
36	27.81860172160403	22.19189586395129	23.262649590594165	26.726852823850518
37	26.411925257190845	21.750997270627757	24.795297081671215	27.041780390510183
38	26.915809363846314	20.260340121771993	25.65609909720764	27.167751417174053
39	26.05500734830989	19.525509132899433	26.348939743858914	28.070543774931767
40	25.19420533277346	21.100146966197777	26.93680453495696	26.768843166071804
41	23.38862061725803	20.659248372874238	27.923577577157253	28.028553432710478
42	21.750997270627757	20.659248372874238	28.74238925047239	28.847365106025613
43	21.5830359017426	20.281335292882638	28.679403737140458	29.456225068234303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	2.5
14	4.0
15	6.0
16	8.0
17	8.5
18	9.0
19	9.0
20	7.5
21	6.0
22	7.0
23	8.0
24	7.5
25	7.0
26	7.0
27	8.5
28	10.0
29	15.0
30	20.0
31	22.0
32	24.0
33	24.0
34	28.5
35	33.0
36	50.0
37	67.0
38	84.5
39	102.0
40	102.0
41	122.0
42	142.0
43	160.5
44	179.0
45	248.0
46	317.0
47	317.0
48	354.0
49	391.0
50	439.0
51	487.0
52	511.0
53	535.0
54	535.0
55	513.0
56	491.0
57	478.5
58	466.0
59	434.0
60	402.0
61	402.0
62	372.0
63	342.0
64	279.0
65	216.0
66	186.5
67	157.0
68	157.0
69	136.5
70	116.0
71	99.5
72	83.0
73	65.5
74	48.0
75	48.0
76	41.5
77	35.0
78	30.0
79	25.0
80	16.0
81	7.0
82	7.0
83	9.5
84	12.0
85	7.5
86	3.0
87	3.5
88	4.0
89	4.0
90	2.5
91	1.0
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4763.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.04597942473231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.96816208393632	80.05458744488767
2	4.8962855764592375	8.524039470921688
3	1.7607332368547997	4.597942473231157
4	0.6271104679208876	2.1834977955070336
5	0.241196333815726	1.0497585555322275
6	0.1929570670525808	1.0077682133109385
7	0.0723589001447178	0.4408985933235356
8	0.0723589001447178	0.5038841066554692
9	0.0723589001447178	0.5668696199874029
>10	0.0964785335262904	1.0707537266428722
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	19	0.3989082511022464	TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	12	0.2519420533277346	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	10	0.20995171110644553	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	10	0.20995171110644553	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	9	0.18895653999580095	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	9	0.18895653999580095	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	9	0.18895653999580095	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	8	0.1679613688851564	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	8	0.1679613688851564	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	8	0.1679613688851564	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	7	0.14696619777451186	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	7	0.14696619777451186	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	7	0.14696619777451186	No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG	6	0.1259710266638673	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	6	0.1259710266638673	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	6	0.1259710266638673	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	6	0.1259710266638673	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	6	0.1259710266638673	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	6	0.1259710266638673	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	6	0.1259710266638673	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	6	0.1259710266638673	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	5	0.10497585555322277	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.10497585555322277	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	5	0.10497585555322277	Illumina Paired End PCR Primer 2 (95% over 23bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	5	0.10497585555322277	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.10497585555322277	No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA	5	0.10497585555322277	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	5	0.10497585555322277	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	5	0.10497585555322277	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	5	0.10497585555322277	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	5	0.10497585555322277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.020995171110644553	0.0
20	0.0	0.0	0.0	0.020995171110644553	0.0
21	0.0	0.0	0.0	0.020995171110644553	0.0
22	0.0	0.0	0.0	0.020995171110644553	0.0
23	0.0	0.0	0.0	0.020995171110644553	0.0
24	0.0	0.0	0.0	0.020995171110644553	0.0
25	0.0	0.0	0.0	0.020995171110644553	0.0
26	0.0	0.0	0.0	0.020995171110644553	0.0
27	0.0	0.0	0.0	0.06298551333193365	0.0
28	0.0	0.0	0.0	0.06298551333193365	0.0
29	0.0	0.0	0.0	0.16796136888515642	0.0
30	0.0	0.0	0.0	0.2729372244383792	0.0
31	0.0	0.0	0.0	0.7348309888725593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTCT	25	0.004969763	29.6	37
>>END_MODULE