FastQCFastQC Report
Fri 10 Feb 2017
ERR1632381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13715
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA570.41560335399197956No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT430.3135253372220197TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA360.2624863288370397No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA310.22602989427633977No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA270.1968647466277798No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC260.18957345971563982No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA230.16769959897921985No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT220.16040831206707984No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA210.15311702515493983No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA210.15311702515493983No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC190.13853445133065986No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT190.13853445133065986No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA180.13124316441851985No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC180.13124316441851985No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA180.13124316441851985No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT180.13124316441851985No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG180.13124316441851985No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC170.12395187750637987No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT160.11666059059423989No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC150.10936930368209989No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC150.10936930368209989No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC150.10936930368209989No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA150.10936930368209989No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC140.1020780167699599No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG140.1020780167699599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA200.001779192837.023
AGCCGCG200.001779192837.019
CGGTAAT200.001779192837.024
CAGCAGC250.005311218629.615
CCAGCAG250.005311218629.614
AGCAGCC250.005311218629.616
GCAGCCG250.005311218629.617
GCCGCGG250.005311218629.620
CCGCGGT250.005311218629.621
GTGCCAG250.005311218629.611
Produced by FastQC (version 0.11.5)