##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13715
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97710535909588	34.0	33.0	34.0	31.0	34.0
2	33.10565074735691	34.0	33.0	34.0	31.0	34.0
3	33.19030258840685	34.0	33.0	34.0	31.0	34.0
4	36.486037185563255	37.0	37.0	37.0	35.0	37.0
5	36.46496536638717	37.0	37.0	37.0	35.0	37.0
6	36.49012030623405	37.0	37.0	37.0	35.0	37.0
7	36.48822457163689	37.0	37.0	37.0	35.0	37.0
8	36.47867298578199	37.0	37.0	37.0	35.0	37.0
9	38.275173168064164	39.0	39.0	39.0	37.0	39.0
10	38.29755741888443	39.0	39.0	39.0	37.0	39.0
11	38.36303317535545	39.0	39.0	39.0	37.0	39.0
12	38.340430185927815	39.0	39.0	39.0	37.0	39.0
13	38.36033539919796	39.0	39.0	39.0	37.0	39.0
14	39.8916514764856	41.0	40.0	41.0	38.0	41.0
15	39.88487057965731	41.0	40.0	41.0	38.0	41.0
16	39.836529347429824	41.0	40.0	41.0	38.0	41.0
17	39.852934742982136	41.0	40.0	41.0	38.0	41.0
18	39.85074735690849	41.0	40.0	41.0	38.0	41.0
19	39.866569449507836	41.0	40.0	41.0	38.0	41.0
20	39.83973751367116	41.0	40.0	41.0	38.0	41.0
21	39.81954064892454	41.0	40.0	41.0	38.0	41.0
22	39.800729128691216	41.0	40.0	41.0	38.0	41.0
23	39.720379146919434	41.0	40.0	41.0	38.0	41.0
24	39.69216186656945	41.0	40.0	41.0	38.0	41.0
25	39.66037185563252	41.0	40.0	41.0	37.0	41.0
26	39.578563616478306	41.0	40.0	41.0	37.0	41.0
27	39.47604812249362	41.0	40.0	41.0	37.0	41.0
28	39.43113379511484	41.0	39.0	41.0	37.0	41.0
29	39.41808239154211	41.0	39.0	41.0	37.0	41.0
30	39.387677725118486	41.0	39.0	41.0	36.0	41.0
31	39.30958804228946	41.0	39.0	41.0	36.0	41.0
32	39.261611374407586	41.0	39.0	41.0	36.0	41.0
33	39.20984323733139	41.0	39.0	41.0	35.0	41.0
34	39.13277433467007	41.0	39.0	41.0	35.0	41.0
35	39.064746627779805	41.0	39.0	41.0	35.0	41.0
36	39.01100984323733	41.0	39.0	41.0	35.0	41.0
37	38.99117754283631	41.0	39.0	41.0	35.0	41.0
38	38.88851622311338	41.0	39.0	41.0	35.0	41.0
39	38.766460080204155	40.0	38.0	41.0	35.0	41.0
40	38.64681006197594	40.0	38.0	41.0	35.0	41.0
41	38.59715639810427	40.0	38.0	41.0	35.0	41.0
42	38.5437841779074	40.0	38.0	41.0	35.0	41.0
43	37.791031717098065	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	3.0
23	9.0
24	5.0
25	15.0
26	24.0
27	30.0
28	48.0
29	56.0
30	76.0
31	73.0
32	114.0
33	185.0
34	247.0
35	314.0
36	545.0
37	1011.0
38	2570.0
39	8385.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.463361283266494	15.873131607728762	12.606635071090047	28.056872037914694
2	21.29784907036092	19.78855267954794	31.345242435289826	27.56835581480131
3	21.625956981407217	19.43857090776522	28.844331024425813	30.091141086401752
4	15.953335763762306	15.829383886255924	32.883703973751366	35.333576376230404
5	17.105359095880424	32.898286547575644	33.605541378053225	16.390812978490704
6	35.2242070725483	33.24826831935837	14.538826102807146	16.98869850528618
7	29.72657674079475	28.392271235873128	19.33649289099526	22.544659132336857
8	28.26831935836675	30.23696682464455	20.26248632883704	21.232227488151658
9	27.69959897921983	12.876412686839227	16.59496901203062	42.829019321910316
10	20.11666059059424	23.069631790010938	29.026613197229313	27.787094422165517
11	39.65730951512942	19.00838497994896	18.5052861830113	22.82901932191032
12	23.179001093693035	23.776886620488515	25.089318264673715	27.95479402114473
13	34.91797302223843	16.966824644549764	20.780167699598977	27.335034633612832
14	24.907036091870218	18.7969376594969	22.034269048487058	34.26175720014582
15	29.814072183740432	24.053955523149835	19.066715275246082	27.065257017863654
16	26.474662777980313	23.959168793292015	21.735326285089318	27.83084214363835
17	27.70689026613197	23.674808603718557	20.524972657674077	28.093328472475392
18	26.445497630331754	21.567626686110096	22.639445862194677	29.34742982136347
19	28.01312431644185	22.3696682464455	22.952971199416698	26.664236237695953
20	29.916150200510387	20.590594239883337	22.413415967918336	27.079839591687932
21	27.816259569814072	21.997812613926357	21.03536274152388	29.15056507473569
22	28.698505286183014	21.691578563616478	20.6926722566533	28.917243893547212
23	28.16624134159679	21.764491432737877	22.333211811884798	27.73605541378053
24	28.63288370397375	21.604083120670797	21.764491432737877	27.998541742617572
25	29.843237331388988	20.83120670798396	22.391542107181916	26.934013853445133
26	29.42763397739701	21.990521327014218	20.93328472475392	27.648559970834853
27	26.53299307327743	21.84469558877142	23.609187021509296	28.01312431644185
28	26.25592417061611	22.311337951148378	22.770689026613198	28.662048851622313
29	27.20379146919431	23.740430185927817	21.42909223477944	27.62668611009843
30	26.45278891724389	22.8800583302953	23.15712723295662	27.510025519504193
31	28.34123222748815	22.391542107181916	22.136347065257016	27.13087860007291
32	26.474662777980313	21.29784907036092	23.01859278162596	29.20889537003281
33	26.955887714181554	20.99161502005104	24.090411957710536	27.96208530805687
34	26.890266131972297	21.851986875683558	23.397739701057237	27.860007291286916
35	26.438206343419612	22.872767043383156	24.892453518045933	25.79657309515129
36	27.04338315712723	21.36347065257018	24.484141450966096	27.109004739336495
37	28.020415603353992	21.00619759387532	24.243528982865474	26.729857819905213
38	26.277798031352535	21.275975209624498	25.257017863652937	27.189208895370033
39	25.344513306598614	20.634341961356178	25.840320816624136	28.180823915421072
40	25.585125774699236	20.61246810061976	26.882974845060154	26.919431279620852
41	23.281079110462997	20.27706890266132	28.29019321910317	28.151658767772513
42	22.770689026613198	20.73641997812614	28.87349617207437	27.619394823186294
43	21.97593875318994	20.72183740430186	29.46409041195771	27.838133430550492
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.5
9	3.0
10	4.0
11	5.0
12	5.0
13	4.5
14	4.0
15	8.5
16	13.0
17	15.5
18	18.0
19	18.0
20	17.5
21	17.0
22	12.5
23	8.0
24	13.5
25	19.0
26	19.0
27	18.5
28	18.0
29	34.0
30	50.0
31	62.0
32	74.0
33	74.0
34	87.5
35	101.0
36	131.5
37	162.0
38	231.5
39	301.0
40	301.0
41	358.0
42	415.0
43	484.5
44	554.0
45	721.5
46	889.0
47	889.0
48	1041.5
49	1194.0
50	1350.0
51	1506.0
52	1609.0
53	1712.0
54	1712.0
55	1560.5
56	1409.0
57	1330.5
58	1252.0
59	1172.5
60	1093.0
61	1093.0
62	1007.5
63	922.0
64	775.5
65	629.0
66	532.5
67	436.0
68	436.0
69	400.0
70	364.0
71	290.0
72	216.0
73	171.0
74	126.0
75	126.0
76	107.5
77	89.0
78	70.0
79	51.0
80	40.0
81	29.0
82	29.0
83	25.0
84	21.0
85	14.0
86	7.0
87	4.5
88	2.0
89	2.0
90	1.5
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	13715.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.36274152387897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.37422600619195	65.84761210353628
2	6.8014705882352935	10.25154939846883
3	2.4380804953560373	5.512212905577835
4	1.2093653250773995	3.645643456069996
5	0.5708204334365325	2.150929639081298
6	0.3289473684210526	1.4874225300765587
7	0.2225232198142415	1.173897192854539
8	0.2515479876160991	1.5165876777251186
9	0.1644736842105263	1.115566897557419
>10	0.6288699690402476	6.8829748450601524
>50	0.009674922600619196	0.41560335399197956
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	57	0.41560335399197956	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	43	0.3135253372220197	TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	36	0.2624863288370397	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	31	0.22602989427633977	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	27	0.1968647466277798	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	26	0.18957345971563982	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	23	0.16769959897921985	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	22	0.16040831206707984	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	21	0.15311702515493983	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	21	0.15311702515493983	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	19	0.13853445133065986	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	19	0.13853445133065986	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	18	0.13124316441851985	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	18	0.13124316441851985	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	18	0.13124316441851985	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	18	0.13124316441851985	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	18	0.13124316441851985	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	17	0.12395187750637987	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	16	0.11666059059423989	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	15	0.10936930368209989	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	15	0.10936930368209989	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	15	0.10936930368209989	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	15	0.10936930368209989	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	14	0.1020780167699599	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	14	0.1020780167699599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.007291286912139993	0.0
28	0.0	0.0	0.0	0.10936930368209989	0.0
29	0.0	0.0	0.0	0.2551950419248997	0.0
30	0.0	0.0	0.0	0.47393364928909953	0.0
31	0.0	0.0	0.0	0.9332847247539191	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTAA	20	0.0017791928	37.0	23
AGCCGCG	20	0.0017791928	37.0	19
CGGTAAT	20	0.0017791928	37.0	24
CAGCAGC	25	0.0053112186	29.6	15
CCAGCAG	25	0.0053112186	29.6	14
AGCAGCC	25	0.0053112186	29.6	16
GCAGCCG	25	0.0053112186	29.6	17
GCCGCGG	25	0.0053112186	29.6	20
CCGCGGT	25	0.0053112186	29.6	21
GTGCCAG	25	0.0053112186	29.6	11
>>END_MODULE