FastQCFastQC Report
Fri 10 Feb 2017
ERR1632380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8145
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA180.22099447513812157No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA170.20871700429711482No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA160.19643953345610804No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA160.19643953345610804No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA150.1841620626151013No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA150.1841620626151013No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA140.17188459177409454No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA140.17188459177409454No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT130.1596071209330878No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA130.1596071209330878No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA120.14732965009208102No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA120.14732965009208102No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT110.13505217925107427RNA PCR Primer, Index 39 (95% over 23bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC110.13505217925107427No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC110.13505217925107427No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA110.13505217925107427No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT100.12277470841006752No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT100.12277470841006752No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG100.12277470841006752No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT100.12277470841006752No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG100.12277470841006752No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG100.12277470841006752No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT90.11049723756906078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11049723756906078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90.11049723756906078No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG90.11049723756906078No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG90.11049723756906078No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC90.11049723756906078No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT90.11049723756906078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTT453.402265E-628.77777737
TGTCTCT603.1737116E-521.58333436
CTGTCTC950.001068746613.63157935
Produced by FastQC (version 0.11.5)