FastQCFastQC Report
Fri 10 Feb 2017
ERR1632378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15714
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA410.2609138347969963No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA350.22273132238767976No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA350.22273132238767976No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA290.18454880997836326No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT290.18454880997836326No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG290.18454880997836326No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC280.17818505791014383No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA260.16545755377370497No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA250.15909380170548557No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC250.15909380170548557No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA250.15909380170548557No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG240.15273004963726614No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT240.15273004963726614TruSeq Adapter, Index 1 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT230.1463662975690467No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.1463662975690467No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG220.14000254550082727No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA220.14000254550082727No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT210.13363879343260787No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC210.13363879343260787No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT210.13363879343260787No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG200.12727504136438844No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC190.12091128929616902No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA190.12091128929616902No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA190.12091128929616902No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG180.11454753722794961No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC180.11454753722794961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180.11454753722794961No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC180.11454753722794961No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG180.11454753722794961No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA180.11454753722794961No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG170.10818378515973018No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG170.10818378515973018No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT160.10182003309151075No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC160.10182003309151075No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT160.10182003309151075No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC160.10182003309151075No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC160.10182003309151075No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA160.10182003309151075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251.17905685E-437.01
GTATCAA303.441721E-430.8333322
AACGCAG450.003662752220.5555557
ACGCAGA450.003662752220.5555558
CAGAGTA450.003662752220.55555511
AGAGTAC450.003662752220.55555512
CGCAGAG450.003662752220.5555559
TATCAAC450.003662752220.5555553
ATCAACG450.003662752220.5555554
CAACGCA450.003662752220.5555556
TCAACGC500.00673786118.55
GCAGAGT500.00673786118.510
TCTCTTA1058.227969E-615.85714237
GTCTCTT1502.3531038E-411.136
TGTCTCT1900.00207009388.76315835
CTGTCTC2553.894173E-58.70588237
Produced by FastQC (version 0.11.5)