##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15714
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91860761104747	34.0	33.0	34.0	31.0	34.0
2	33.06961944762632	34.0	33.0	34.0	31.0	34.0
3	33.16138475245005	34.0	33.0	34.0	31.0	34.0
4	36.50534555173731	37.0	37.0	37.0	35.0	37.0
5	36.42019854906453	37.0	37.0	37.0	35.0	37.0
6	36.465890288914345	37.0	37.0	37.0	35.0	37.0
7	36.48256331933308	37.0	37.0	37.0	35.0	37.0
8	36.44877179585083	37.0	37.0	37.0	35.0	37.0
9	38.19033982436044	39.0	39.0	39.0	37.0	39.0
10	38.211531118747615	39.0	39.0	39.0	37.0	39.0
11	38.299796359933815	39.0	39.0	39.0	37.0	39.0
12	38.266641211658396	39.0	39.0	39.0	37.0	39.0
13	38.27039582537864	39.0	39.0	39.0	37.0	39.0
14	39.824296805396465	41.0	40.0	41.0	38.0	41.0
15	39.81436935217004	41.0	40.0	41.0	38.0	41.0
16	39.75251368206695	41.0	40.0	41.0	38.0	41.0
17	39.778477790505285	41.0	40.0	41.0	38.0	41.0
18	39.76263204785541	41.0	40.0	41.0	38.0	41.0
19	39.71267659411989	41.0	40.0	41.0	38.0	41.0
20	39.70179457808324	41.0	40.0	41.0	38.0	41.0
21	39.722285859742904	41.0	40.0	41.0	38.0	41.0
22	39.65858470154003	41.0	40.0	41.0	38.0	41.0
23	39.63312969326715	41.0	40.0	41.0	37.0	41.0
24	39.632684230622374	41.0	40.0	41.0	37.0	41.0
25	39.548555428280515	41.0	40.0	41.0	37.0	41.0
26	39.49656357388316	41.0	39.0	41.0	37.0	41.0
27	39.40448008145603	41.0	39.0	41.0	37.0	41.0
28	39.381888761613844	41.0	39.0	41.0	37.0	41.0
29	39.30889652539137	41.0	39.0	41.0	36.0	41.0
30	39.25391370752195	41.0	39.0	41.0	36.0	41.0
31	39.1601120020364	40.0	39.0	41.0	36.0	41.0
32	39.13357515591193	40.0	39.0	41.0	36.0	41.0
33	39.06045564464809	40.0	39.0	41.0	35.0	41.0
34	39.01826396843579	40.0	39.0	41.0	35.0	41.0
35	38.947371770395826	40.0	39.0	41.0	35.0	41.0
36	38.85579737813415	40.0	38.0	41.0	35.0	41.0
37	38.82423316787578	40.0	38.0	41.0	35.0	41.0
38	38.73717703958254	40.0	38.0	41.0	35.0	41.0
39	38.640766195749016	40.0	38.0	41.0	35.0	41.0
40	38.55192821687667	40.0	38.0	41.0	35.0	41.0
41	38.48612702049128	40.0	38.0	41.0	35.0	41.0
42	38.46487208858343	40.0	38.0	41.0	35.0	41.0
43	37.689576174112254	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	0.0
20	3.0
21	2.0
22	2.0
23	4.0
24	13.0
25	16.0
26	22.0
27	23.0
28	56.0
29	47.0
30	93.0
31	96.0
32	138.0
33	175.0
34	302.0
35	399.0
36	704.0
37	1360.0
38	3651.0
39	8606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80896016291205	16.469390352551866	12.848415425735013	27.87323405880107
2	21.878579610538374	19.313987527045946	29.756904670994018	29.05052819142166
3	21.974035891561662	18.569428535064276	28.49688176148657	30.959653811887488
4	16.412116583937888	15.075728649611811	32.00330915107548	36.508845615374824
5	17.93941708031055	31.7487590683467	32.283314242077125	18.028509609265623
6	36.165203003690976	31.958762886597935	14.76390479826906	17.112129311442025
7	31.411480208731067	26.466844851724574	19.313987527045946	22.80768741249841
8	29.254168257604686	29.126893216240298	19.38398879979636	22.23494972635866
9	28.29324169530355	12.593865343006236	17.95850833651521	41.154384625175005
10	20.60582919689449	23.1004200076365	28.961435662466588	27.332315133002417
11	40.28891434389716	19.377625047728138	17.264859361079292	23.068601247295405
12	24.697721776759575	22.604047346315387	25.270459462899325	27.42777141402571
13	35.967926689576174	16.921216749395445	20.16673030418735	26.944126256841034
14	24.697721776759575	19.69581265113911	21.916762122947688	33.68970344915362
15	29.7505409189258	23.838615247549953	19.072164948453608	27.33867888507064
16	26.68321242204404	23.055873743158966	21.57948326333206	28.681430571464933
17	27.63141148020873	23.34860633829706	20.548555428280515	28.471426753213695
18	27.052310042000766	20.612192948962708	22.667684866997583	29.667812142038947
19	29.184166984854272	21.522209494718087	22.317678503245514	26.97594501718213
20	31.023291332569684	20.128547791778033	21.662212040218915	27.185948835433372
21	28.789614356624664	21.044928089601626	21.59857451953672	28.566883034236984
22	29.86508845615375	21.401298205421917	20.624920453099147	28.108692885325187
23	29.037800687285227	20.962199312714777	21.299478172330407	28.700521827669593
24	28.560519282168766	20.72037673412244	22.954053710067456	27.76505027364134
25	29.400534555173728	21.178566883034236	21.636757031946036	27.784141529845996
26	28.649611811123837	22.438589792541684	21.738577065037546	27.173221331296933
27	26.96321751304569	21.25493190785287	24.042255313732976	27.73959526536846
28	27.478681430571466	22.190403461881125	21.102201858215604	29.228713249331804
29	27.936871579483263	23.068601247295405	21.388570701285477	27.605956471935855
30	28.80870561282932	21.000381825124094	22.355861015654828	27.835051546391753
31	27.936871579483263	22.177675957744686	22.78859615629375	27.0968563064783
32	26.237749777268675	21.324933180603285	22.12040218913071	30.31691485299733
33	26.002290950744563	21.312205676466846	23.69224894998091	28.993254422807684
34	27.21140384370625	21.23584065164821	22.400407280132367	29.152348224513176
35	26.715031182385136	22.406771032200584	23.584065164821176	27.294132620593103
36	26.524118620338548	22.075855924653176	24.56408298332697	26.8359424716813
37	27.147766323024054	20.414916634847906	25.187730686012472	27.249586356115568
38	27.065037546137205	19.61944762632048	25.32136947944508	27.994145348097238
39	25.569555810105637	19.61308387425226	26.517754868270334	28.29960544737177
40	25.11136566119384	19.93763522973145	27.357770141275296	27.593228967799416
41	23.482245131729666	20.128547791778033	27.790505281914218	28.598701794578083
42	22.196767213949347	20.026727758686523	29.400534555173728	28.375970472190403
43	22.438589792541684	21.223113147511775	28.325060455644646	28.013236604301895
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	1.5
9	0.0
10	2.0
11	4.0
12	4.0
13	5.5
14	7.0
15	11.5
16	16.0
17	17.0
18	18.0
19	18.0
20	24.5
21	31.0
22	27.0
23	23.0
24	22.0
25	21.0
26	21.0
27	26.5
28	32.0
29	40.0
30	48.0
31	60.0
32	72.0
33	72.0
34	92.5
35	113.0
36	139.0
37	165.0
38	212.5
39	260.0
40	260.0
41	317.5
42	375.0
43	478.0
44	581.0
45	804.0
46	1027.0
47	1027.0
48	1172.0
49	1317.0
50	1469.0
51	1621.0
52	1689.5
53	1758.0
54	1758.0
55	1647.5
56	1537.0
57	1470.0
58	1403.0
59	1388.0
60	1373.0
61	1373.0
62	1282.0
63	1191.0
64	1020.0
65	849.0
66	773.5
67	698.0
68	698.0
69	608.0
70	518.0
71	415.0
72	312.0
73	232.0
74	152.0
75	152.0
76	116.5
77	81.0
78	62.5
79	44.0
80	36.0
81	28.0
82	28.0
83	24.5
84	21.0
85	15.0
86	9.0
87	7.0
88	5.0
89	5.0
90	2.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	15714.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.0304187348861
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.57406761937958	63.9557082856052
2	6.378529104217498	9.316533027873234
3	1.969327291739282	4.314623902252769
4	1.2547926106657372	3.6655211912943875
5	0.8103868943882885	2.9591447117220313
6	0.3921226908330429	1.718213058419244
7	0.32241199024050193	1.6482117856688305
8	0.2701289647960962	1.5782105129184167
9	0.2178459393516905	1.43184421534937
>10	0.8103868943882885	9.411989308896525
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	41	0.2609138347969963	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	35	0.22273132238767976	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	35	0.22273132238767976	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	29	0.18454880997836326	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	29	0.18454880997836326	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	29	0.18454880997836326	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	28	0.17818505791014383	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	26	0.16545755377370497	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	25	0.15909380170548557	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	25	0.15909380170548557	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	25	0.15909380170548557	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	24	0.15273004963726614	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	24	0.15273004963726614	TruSeq Adapter, Index 1 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	23	0.1463662975690467	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	23	0.1463662975690467	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	22	0.14000254550082727	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	22	0.14000254550082727	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	21	0.13363879343260787	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	21	0.13363879343260787	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	21	0.13363879343260787	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	20	0.12727504136438844	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	19	0.12091128929616902	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	19	0.12091128929616902	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	19	0.12091128929616902	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	18	0.11454753722794961	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	18	0.11454753722794961	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.11454753722794961	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	18	0.11454753722794961	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	18	0.11454753722794961	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	18	0.11454753722794961	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	17	0.10818378515973018	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	17	0.10818378515973018	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	16	0.10182003309151075	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	16	0.10182003309151075	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	16	0.10182003309151075	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	16	0.10182003309151075	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	16	0.10182003309151075	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	16	0.10182003309151075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.006363752068219423	0.0
23	0.0	0.0	0.0	0.006363752068219423	0.0
24	0.0	0.0	0.0	0.012727504136438845	0.0
25	0.0	0.0	0.0	0.012727504136438845	0.0
26	0.0	0.0	0.0	0.02545500827287769	0.0
27	0.0	0.0	0.0	0.05091001654575538	0.0
28	0.0	0.0	0.0	0.21000381825124093	0.0
29	0.0	0.0	0.0	0.36909761995672646	0.0
30	0.0	0.0	0.0	0.6045564464808451	0.0
31	0.0	0.0	0.0	1.2282041491663485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25	1.17905685E-4	37.0	1
GTATCAA	30	3.441721E-4	30.833332	2
AACGCAG	45	0.0036627522	20.555555	7
ACGCAGA	45	0.0036627522	20.555555	8
CAGAGTA	45	0.0036627522	20.555555	11
AGAGTAC	45	0.0036627522	20.555555	12
CGCAGAG	45	0.0036627522	20.555555	9
TATCAAC	45	0.0036627522	20.555555	3
ATCAACG	45	0.0036627522	20.555555	4
CAACGCA	45	0.0036627522	20.555555	6
TCAACGC	50	0.006737861	18.5	5
GCAGAGT	50	0.006737861	18.5	10
TCTCTTA	105	8.227969E-6	15.857142	37
GTCTCTT	150	2.3531038E-4	11.1	36
TGTCTCT	190	0.0020700938	8.763158	35
CTGTCTC	255	3.894173E-5	8.705882	37
>>END_MODULE