FastQCFastQC Report
Fri 10 Feb 2017
ERR1632377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13816
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA540.390851187029531No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT490.3546612623045744Illumina PCR Primer Index 11 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA390.28228141285466124No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA320.23161551823972204No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT280.2026635784597568No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA280.2026635784597568No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT230.16647365373480022No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC230.16647365373480022No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA230.16647365373480022No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA220.15923566878980894No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT210.1519976838448176No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT210.1519976838448176No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC190.13752171395483495No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC180.13028372900984367No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT180.13028372900984367No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT180.13028372900984367No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC180.13028372900984367No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG180.13028372900984367No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC180.13028372900984367No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC170.12304574406485234No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA170.12304574406485234No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC160.11580775911986102No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC150.10856977417486972No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA150.10856977417486972No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA140.1013317892298784No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC140.1013317892298784No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC140.1013317892298784No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC140.1013317892298784No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA140.1013317892298784No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA140.1013317892298784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA250.005312559729.61
GTCTCTT758.228293E-619.73333437
TGTCTCT1102.1926273E-413.45454536
CTGTCTC1502.3132851E-411.137
Produced by FastQC (version 0.11.5)