##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632377.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13816
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.978503184713375	34.0	33.0	34.0	31.0	34.0
2	33.128112333526346	34.0	33.0	34.0	31.0	34.0
3	33.188911407064275	34.0	33.0	34.0	31.0	34.0
4	36.49261725535611	37.0	37.0	37.0	35.0	37.0
5	36.46576433121019	37.0	37.0	37.0	35.0	37.0
6	36.50571800810654	37.0	37.0	37.0	35.0	37.0
7	36.511146496815286	37.0	37.0	37.0	35.0	37.0
8	36.47184423856398	37.0	37.0	37.0	35.0	37.0
9	38.26708164447018	39.0	39.0	39.0	37.0	39.0
10	38.31007527504343	39.0	39.0	39.0	37.0	39.0
11	38.367906774753905	39.0	39.0	39.0	37.0	39.0
12	38.334033005211346	39.0	39.0	39.0	37.0	39.0
13	38.36197162709902	39.0	39.0	39.0	37.0	39.0
14	39.9145917776491	41.0	40.0	41.0	38.0	41.0
15	39.90583381586566	41.0	40.0	41.0	38.0	41.0
16	39.863274464389114	41.0	40.0	41.0	38.0	41.0
17	39.8326577880718	41.0	40.0	41.0	38.0	41.0
18	39.85610885929357	41.0	40.0	41.0	38.0	41.0
19	39.88375796178344	41.0	40.0	41.0	38.0	41.0
20	39.84286334684424	41.0	40.0	41.0	38.0	41.0
21	39.80348870874349	41.0	40.0	41.0	38.0	41.0
22	39.78257093225246	41.0	40.0	41.0	38.0	41.0
23	39.743485813549505	41.0	40.0	41.0	38.0	41.0
24	39.72119281991893	41.0	40.0	41.0	38.0	41.0
25	39.6680660104227	41.0	40.0	41.0	37.0	41.0
26	39.571366531557615	41.0	40.0	41.0	37.0	41.0
27	39.46554719166184	41.0	39.0	41.0	37.0	41.0
28	39.42392877822814	41.0	39.0	41.0	37.0	41.0
29	39.36747249565721	41.0	39.0	41.0	36.0	41.0
30	39.385712217718584	41.0	39.0	41.0	36.0	41.0
31	39.28336711059641	41.0	39.0	41.0	36.0	41.0
32	39.22314707585408	41.0	39.0	41.0	36.0	41.0
33	39.152359583092064	41.0	39.0	41.0	35.0	41.0
34	39.08757961783439	41.0	39.0	41.0	35.0	41.0
35	39.02743196294152	41.0	39.0	41.0	35.0	41.0
36	38.963520555877245	41.0	39.0	41.0	35.0	41.0
37	38.92660683265779	40.0	39.0	41.0	35.0	41.0
38	38.83157209033005	40.0	39.0	41.0	35.0	41.0
39	38.715330052113494	40.0	38.0	41.0	35.0	41.0
40	38.670671685002894	40.0	38.0	41.0	35.0	41.0
41	38.61566299942096	40.0	38.0	41.0	35.0	41.0
42	38.560437174290676	40.0	38.0	41.0	35.0	41.0
43	37.77634626519977	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	1.0
21	2.0
22	2.0
23	5.0
24	12.0
25	16.0
26	27.0
27	21.0
28	33.0
29	44.0
30	73.0
31	93.0
32	137.0
33	154.0
34	232.0
35	347.0
36	585.0
37	1081.0
38	2567.0
39	8382.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.50752750434279	16.234800231615516	12.290098436595253	27.96757382744644
2	21.4171974522293	19.60770121598147	31.130573248407643	27.844528083381586
3	21.844238563983787	19.253039953676897	28.249855240301102	30.65286624203822
4	15.880138969310945	15.69918934568616	32.71569195136074	35.704979733642155
5	17.327735958309205	32.41169658367111	33.070353213665314	17.190214244354372
6	35.379270411117545	32.67950202663579	15.091198610306892	16.85002895193978
7	30.052113491603937	27.757672264041688	18.949044585987263	23.241169658367113
8	27.627388535031848	30.616676317313257	20.04921829762594	21.70671685002895
9	27.410248986682106	13.107990735379271	17.038216560509554	42.443543717429066
10	20.251881876085697	23.103647944412277	28.047191661841342	28.597278517660683
11	39.20092646207296	19.043138390272148	18.073248407643312	23.68268674001158
12	23.01679212507238	23.718876664736538	25.477707006369428	27.78662420382166
13	35.86421540243197	16.227562246670526	21.113202084539665	26.795020266357845
14	24.40648523451071	19.752460914881297	22.394325419803128	33.44672843080486
15	29.494788650839602	23.711638679791548	19.701795020266356	27.09177764910249
16	26.09293572669369	23.56687898089172	22.0830920671685	28.257093225246095
17	27.996525767226405	23.29183555298205	21.31586566299942	27.395773016792123
18	26.25940938042849	20.715112912565143	23.588592935726695	29.436884771279676
19	28.62623045744065	21.894904458598727	22.589751013317894	26.889114070642734
20	29.719166184134338	20.693398957730167	22.30023161551824	27.287203242617252
21	27.46091488129705	21.69947886508396	21.31586566299942	29.523740590619575
22	28.720324261725537	22.19166184134337	20.53416328894036	28.553850607990732
23	28.86508396062536	21.31586566299942	21.692240880138968	28.12680949623625
24	28.30052113491604	21.20005790387956	22.358135495078173	28.14128546612623
25	29.378980891719745	21.055298204979735	22.0830920671685	27.482628836132022
26	28.53213665315576	22.365373480023163	21.395483497394324	27.70700636942675
27	26.787782281412852	22.13375796178344	23.34973943254198	27.728720324261722
28	26.32455124493341	21.713954834973944	22.553561088592936	29.40793283149971
29	27.670816444701796	23.190503763752172	21.837000579038797	27.301679212507242
30	27.323393167342214	21.171105964099596	24.044585987261147	27.46091488129705
31	28.669658367110596	21.504053271569195	22.0830920671685	27.743196294151705
32	26.4186450492183	21.460625361899247	22.51737116386798	29.603358425014477
33	27.077301679212507	21.14215402431963	23.414881297046904	28.36566299942096
34	26.838448176027796	21.35205558772438	22.872032426172552	28.937463810075275
35	25.85408222350897	23.371453387376953	23.88535031847134	26.889114070642734
36	27.156919513607413	21.80081065431384	23.856398378691374	27.185871453387378
37	27.714244354371743	21.344817602779386	24.11696583671106	26.823972206137814
38	26.35350318471338	20.910538506079906	25.513896931094383	27.222061378112333
39	25.702084539664156	20.360451650260565	26.042269832078752	27.895193977996524
40	24.84800231615518	20.59206716850029	27.87348002316155	26.686450492182978
41	23.35697741748697	20.4183555298205	27.68529241459178	28.53937463810075
42	22.763462651997685	20.903300521134916	28.894035900405328	27.43920092646207
43	21.894904458598727	20.773016792125073	28.242617255356105	29.089461493920094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	2.5
4	4.0
5	4.0
6	2.0
7	0.0
8	0.5
9	1.0
10	0.5
11	0.0
12	0.0
13	5.0
14	10.0
15	10.5
16	11.0
17	14.0
18	17.0
19	17.0
20	16.0
21	15.0
22	14.0
23	13.0
24	17.5
25	22.0
26	22.0
27	26.0
28	30.0
29	39.5
30	49.0
31	64.0
32	79.0
33	79.0
34	94.0
35	109.0
36	134.0
37	159.0
38	216.0
39	273.0
40	273.0
41	330.5
42	388.0
43	438.0
44	488.0
45	702.5
46	917.0
47	917.0
48	1050.0
49	1183.0
50	1358.0
51	1533.0
52	1606.0
53	1679.0
54	1679.0
55	1546.5
56	1414.0
57	1315.0
58	1216.0
59	1187.5
60	1159.0
61	1159.0
62	1081.5
63	1004.0
64	797.5
65	591.0
66	551.0
67	511.0
68	511.0
69	424.0
70	337.0
71	287.0
72	237.0
73	198.0
74	159.0
75	159.0
76	123.5
77	88.0
78	71.0
79	54.0
80	42.5
81	31.0
82	31.0
83	24.0
84	17.0
85	13.0
86	9.0
87	6.0
88	3.0
89	3.0
90	2.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	13816.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.65749855240301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.5657633840053	67.89229878401854
2	5.995656689642149	9.192240880138968
3	2.1338872627702767	4.907353792704111
4	1.1991313379284299	3.676896352055588
5	0.566518742328392	2.1713954834973945
6	0.4154470777074875	1.910828025477707
7	0.3304692663582287	1.773306311522872
8	0.1699556226985176	1.0422698320787493
9	0.11330374846567841	0.781702374059062
>10	0.5004248890567463	6.260856977417487
>50	0.009441979038806534	0.390851187029531
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	54	0.390851187029531	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	49	0.3546612623045744	Illumina PCR Primer Index 11 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	39	0.28228141285466124	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	32	0.23161551823972204	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	28	0.2026635784597568	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	28	0.2026635784597568	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	23	0.16647365373480022	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	23	0.16647365373480022	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	23	0.16647365373480022	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	22	0.15923566878980894	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	21	0.1519976838448176	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	21	0.1519976838448176	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	19	0.13752171395483495	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	18	0.13028372900984367	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	18	0.13028372900984367	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	18	0.13028372900984367	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	18	0.13028372900984367	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	18	0.13028372900984367	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	18	0.13028372900984367	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	17	0.12304574406485234	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	17	0.12304574406485234	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	16	0.11580775911986102	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	15	0.10856977417486972	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	15	0.10856977417486972	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	14	0.1013317892298784	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	14	0.1013317892298784	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	14	0.1013317892298784	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	14	0.1013317892298784	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	14	0.1013317892298784	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	14	0.1013317892298784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.007237984944991315	0.0
18	0.0	0.0	0.0	0.007237984944991315	0.0
19	0.0	0.0	0.0	0.007237984944991315	0.0
20	0.0	0.0	0.0	0.007237984944991315	0.0
21	0.0	0.0	0.0	0.007237984944991315	0.0
22	0.0	0.0	0.0	0.007237984944991315	0.0
23	0.0	0.0	0.0	0.007237984944991315	0.0
24	0.0	0.0	0.0	0.01447596988998263	0.0
25	0.0	0.0	0.0	0.01447596988998263	0.0
26	0.0	0.0	0.0	0.01447596988998263	0.0
27	0.0	0.0	0.0	0.04342790966994789	0.0
28	0.0	0.0	0.0	0.18818760856977418	0.0
29	0.0	0.0	0.0	0.36189924724956574	0.0
30	0.0	0.0	0.0	0.5138969310943833	0.0
31	0.0	0.0	0.0	0.9047481181239143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	25	0.0053125597	29.6	1
GTCTCTT	75	8.228293E-6	19.733334	37
TGTCTCT	110	2.1926273E-4	13.454545	36
CTGTCTC	150	2.3132851E-4	11.1	37
>>END_MODULE