FastQCFastQC Report
Fri 10 Feb 2017
ERR1632376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36980
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1670.4515954570037858No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1650.44618712817739314No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1320.35694970254191455No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1250.33802055164954026No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1190.32179556517036234No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1140.30827474310438074No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1060.28664142779881013No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1030.27852893455922123No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1020.2758247701460249No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT910.24607896160086534No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC880.23796646836127638No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG860.23255813953488372No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC850.2298539751216874No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC850.2298539751216874No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT820.2217414818820984No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA790.21362898864250948No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC740.20010816657652786No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT720.19469983775013522No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT680.1838831800973499No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA680.1838831800973499No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT670.1811790156841536No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC670.1811790156841536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT660.17847485127095727No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC660.17847485127095727No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC660.17847485127095727No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC660.17847485127095727No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC650.17577068685776095No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC640.17306652244456464No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT640.17306652244456464No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA610.16495402920497568No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC600.16224986479177933No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA570.15413737155219037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT560.15143320713899405No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT550.14872904272579773No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC550.14872904272579773No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA550.14872904272579773No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA550.14872904272579773No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT540.1460248783126014No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC540.1460248783126014No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG540.1460248783126014No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA530.14332071389940507No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA530.14332071389940507No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC500.1352082206598161No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA500.1352082206598161No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA480.12979989183342347No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA470.12709572742022715No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG470.12709572742022715No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC470.12709572742022715No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG460.12439156300703083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT460.12439156300703083No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA450.12168739859383451No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC440.11898323418063819No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG440.11898323418063819No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA440.11898323418063819No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC440.11898323418063819No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG440.11898323418063819No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT430.11627906976744186No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTC430.11627906976744186No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT430.11627906976744186No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG430.11627906976744186No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG420.11357490535424553No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC420.11357490535424553No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT410.1108707409410492No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA410.1108707409410492No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC410.1108707409410492No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG410.1108707409410492No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG410.1108707409410492No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA410.1108707409410492No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC380.10275824770146025No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT380.10275824770146025No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA380.10275824770146025No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA370.10005408328826393No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG370.10005408328826393No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC370.10005408328826393No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA370.10005408328826393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA200.001818979537.037
GGTATCA751.2732926E-1129.61
TGTGACG250.005428622529.59999812
TATCGCG250.005428622529.5999987
ATCGCGC250.005428622529.5999988
GTGTGAC250.005428622529.59999811
GCAGCCG706.162736E-926.42857217
AGCCGCG706.162736E-926.42857219
CAGCCGC706.162736E-926.42857218
CAGCAGC751.2845703E-824.66666615
TGCCAGC751.2845703E-824.66666612
CGTGCCA751.2845703E-824.66666610
GCCGCGG851.8244464E-923.94117720
GTGCCAG851.8244464E-923.94117711
GCGGTAA802.5496774E-823.12500223
GCGCGCC400.001896228623.12500211
CGCGGTA802.5496774E-823.12500222
CCGCGGT802.5496774E-823.12500221
AACTCCG753.5424455E-722.25
TAACTCC753.5424455E-722.24
Produced by FastQC (version 0.11.5)