##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36980
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0157382368848	34.0	33.0	34.0	31.0	34.0
2	33.150243374797185	34.0	33.0	34.0	31.0	34.0
3	33.17955651703623	34.0	33.0	34.0	31.0	34.0
4	36.4953488372093	37.0	37.0	37.0	35.0	37.0
5	36.47920497566252	37.0	37.0	37.0	35.0	37.0
6	36.55305570578691	37.0	37.0	37.0	35.0	37.0
7	36.557815035154135	37.0	37.0	37.0	35.0	37.0
8	36.52652785289346	37.0	37.0	37.0	35.0	37.0
9	38.337669010275825	39.0	39.0	39.0	37.0	39.0
10	38.36433207138994	39.0	39.0	39.0	37.0	39.0
11	38.42993510005408	39.0	39.0	39.0	37.0	39.0
12	38.38023255813953	39.0	39.0	39.0	37.0	39.0
13	38.431043807463496	39.0	39.0	39.0	37.0	39.0
14	39.9165765278529	41.0	40.0	41.0	38.0	41.0
15	39.9269875608437	41.0	40.0	41.0	38.0	41.0
16	39.87341806381828	41.0	40.0	41.0	38.0	41.0
17	39.9079502433748	41.0	40.0	41.0	38.0	41.0
18	39.94353704705246	41.0	40.0	41.0	38.0	41.0
19	39.92785289345592	41.0	40.0	41.0	38.0	41.0
20	39.92614926987561	41.0	40.0	41.0	38.0	41.0
21	39.87079502433748	41.0	40.0	41.0	38.0	41.0
22	39.823580313683074	41.0	40.0	41.0	38.0	41.0
23	39.78385613845322	41.0	40.0	41.0	38.0	41.0
24	39.744808004326664	41.0	40.0	41.0	38.0	41.0
25	39.71281773931855	41.0	40.0	41.0	38.0	41.0
26	39.631584640346134	41.0	40.0	41.0	37.0	41.0
27	39.525905895078424	41.0	39.0	41.0	37.0	41.0
28	39.45605732828556	41.0	39.0	41.0	37.0	41.0
29	39.421416982152515	41.0	39.0	41.0	37.0	41.0
30	39.35335316387236	41.0	39.0	41.0	36.0	41.0
31	39.30646295294754	41.0	39.0	41.0	36.0	41.0
32	39.25995132504056	41.0	39.0	41.0	36.0	41.0
33	39.18939967550027	41.0	39.0	41.0	35.0	41.0
34	39.1329096809086	41.0	39.0	41.0	35.0	41.0
35	39.05259599783667	41.0	39.0	41.0	35.0	41.0
36	38.966197944835045	40.0	39.0	41.0	35.0	41.0
37	38.90335316387236	40.0	39.0	41.0	35.0	41.0
38	38.844808004326666	40.0	39.0	41.0	35.0	41.0
39	38.71146565711195	40.0	38.0	41.0	35.0	41.0
40	38.646944294213085	40.0	38.0	41.0	35.0	41.0
41	38.56106003244997	40.0	38.0	41.0	35.0	41.0
42	38.46406165494862	40.0	38.0	41.0	35.0	41.0
43	37.7269064359113	40.0	36.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	2.0
22	11.0
23	16.0
24	13.0
25	38.0
26	60.0
27	51.0
28	85.0
29	115.0
30	177.0
31	219.0
32	295.0
33	412.0
34	553.0
35	946.0
36	1485.0
37	3090.0
38	7397.0
39	22009.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.10978907517577	15.140616549486207	13.988642509464574	27.760951865873444
2	22.379664683612763	19.44023796646836	31.584640346133046	26.59545700378583
3	20.413737155219035	19.202271498107084	30.12168739859383	30.262303948080042
4	14.402379664683613	17.563547863710113	35.33802055164954	32.69605191995673
5	16.371011357490538	32.3499188750676	34.00757166035695	17.27149810708491
6	29.651162790697676	35.01081665765279	17.028123309897243	18.3098972417523
7	28.19091400757166	28.234180638182803	19.710654407787995	23.864250946457545
8	26.606273661438617	29.299621416982152	21.538669551108708	22.555435370470526
9	28.088155759870197	12.049756625202813	17.082206598161168	42.77988101676582
10	20.77879935100054	21.979448350459705	26.53596538669551	30.70578691184424
11	39.5565170362358	19.726879394267172	17.136289886425093	23.580313683071928
12	21.898323418063818	24.929691725256895	25.5137912385073	27.658193618171982
13	38.28015143320714	16.97133585722012	18.90481341265549	25.843699296917254
14	23.24499729583559	21.74148188209843	23.350459707950243	31.663061114115738
15	31.992969172525687	22.56625202812331	19.302325581395348	26.138453217955654
16	23.30178474851271	23.737155219037316	23.55597620335316	29.405083829096807
17	27.71498107084911	23.43969713358572	20.813953488372093	28.031368307193077
18	27.80962682531098	19.394267171444024	22.052460789616006	30.74364521362899
19	30.348837209302324	21.400757166035696	22.268793942671714	25.981611681990262
20	33.34234721471066	18.39102217414819	21.346673877771767	26.91995673336939
21	29.21579232017307	20.48404542996214	20.941049215792322	29.35911303407247
22	30.06489994591671	22.052460789616006	20.135208220659816	27.747431043807463
23	29.30232558139535	20.648999459167115	21.633315305570576	28.415359653866958
24	27.974580854515956	19.164413196322336	23.78853434288805	29.07247160627366
25	29.15359653866955	20.943753380205514	22.598702001081666	27.303948080043266
26	28.31800973499189	22.298539751216875	22.701460248783125	26.68199026500811
27	27.723093564088696	20.48674959437534	25.03785830178475	26.75229853975122
28	26.068144943212545	23.104380746349378	21.40346133044889	29.424012979989183
29	27.750135208220662	24.445646295294754	21.111411573823688	26.6928069226609
30	27.084910762574367	21.38453217955652	24.548404542996213	26.982152514872904
31	28.837209302325583	21.914548404542998	22.482422931314225	26.7658193618172
32	25.532720389399678	21.335857220118985	23.171984856679288	29.959437533802053
33	25.63277447268794	20.886965927528394	25.427257977285016	28.05300162249865
34	26.103299080584097	20.989724175229853	24.75121687398594	28.155759870200107
35	24.91076257436452	23.526230394808003	25.724716062736615	25.83829096809086
36	26.487290427257975	21.30070308274743	26.22228231476474	25.989724175229856
37	26.289886425094643	21.687398593834505	25.532720389399678	26.489994591671174
38	25.143320713899403	19.818820984315845	26.849648458626284	28.18820984315846
39	24.342888047593295	18.766901027582477	28.22877230935641	28.661438615467823
40	23.950784207679828	20.48404542996214	29.53758788534343	26.027582477014604
41	22.44997295835587	19.113034072471606	30.300162249864794	28.13683071930773
42	19.467279610600325	20.348837209302324	32.14440237966468	28.039480800432663
43	18.472147106544078	21.792861005949163	30.76257436452136	28.9724175229854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.0
9	3.0
10	9.5
11	16.0
12	16.0
13	27.0
14	38.0
15	75.5
16	113.0
17	103.0
18	93.0
19	93.0
20	104.0
21	115.0
22	80.0
23	45.0
24	40.5
25	36.0
26	36.0
27	33.5
28	31.0
29	33.5
30	36.0
31	39.0
32	42.0
33	42.0
34	65.5
35	89.0
36	86.5
37	84.0
38	170.0
39	256.0
40	256.0
41	420.5
42	585.0
43	852.0
44	1119.0
45	2172.0
46	3225.0
47	3225.0
48	3752.5
49	4280.0
50	4704.5
51	5129.0
52	5099.0
53	5069.0
54	5069.0
55	4483.5
56	3898.0
57	3639.0
58	3380.0
59	3198.0
60	3016.0
61	3016.0
62	2741.5
63	2467.0
64	1919.0
65	1371.0
66	1172.0
67	973.0
68	973.0
69	784.5
70	596.0
71	517.5
72	439.0
73	330.5
74	222.0
75	222.0
76	165.0
77	108.0
78	83.0
79	58.0
80	45.5
81	33.0
82	33.0
83	21.5
84	10.0
85	6.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	36980.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.527852893455915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4880608764104	41.473769605192
2	8.60666491734453	8.869659275283938
3	3.484649698241931	5.3866955110870745
4	1.794804513251115	3.699296917252569
5	1.1335607452112306	2.9204975662520285
6	0.7924429283652584	2.4499729583558683
7	0.5143007084754658	1.855056787452677
8	0.48281290999737597	1.9902650081124933
9	0.33062188401994225	1.5332612222823148
>10	2.1516662293361324	20.832882639264465
>50	0.17318289162949357	5.924824229313142
>100	0.04723169771713461	3.063818280151433
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	167	0.4515954570037858	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	165	0.44618712817739314	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	132	0.35694970254191455	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	125	0.33802055164954026	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	119	0.32179556517036234	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	114	0.30827474310438074	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	106	0.28664142779881013	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	103	0.27852893455922123	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	102	0.2758247701460249	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	91	0.24607896160086534	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	88	0.23796646836127638	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	86	0.23255813953488372	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	85	0.2298539751216874	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	85	0.2298539751216874	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	82	0.2217414818820984	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	79	0.21362898864250948	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	74	0.20010816657652786	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	72	0.19469983775013522	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	68	0.1838831800973499	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	68	0.1838831800973499	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	67	0.1811790156841536	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	67	0.1811790156841536	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	66	0.17847485127095727	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	66	0.17847485127095727	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	66	0.17847485127095727	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	66	0.17847485127095727	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	65	0.17577068685776095	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	64	0.17306652244456464	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	64	0.17306652244456464	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	61	0.16495402920497568	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	60	0.16224986479177933	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	57	0.15413737155219037	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.15143320713899405	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	55	0.14872904272579773	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	55	0.14872904272579773	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	55	0.14872904272579773	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	55	0.14872904272579773	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	54	0.1460248783126014	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	54	0.1460248783126014	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	54	0.1460248783126014	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	53	0.14332071389940507	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	53	0.14332071389940507	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	50	0.1352082206598161	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	50	0.1352082206598161	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	48	0.12979989183342347	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	47	0.12709572742022715	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	47	0.12709572742022715	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	47	0.12709572742022715	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	46	0.12439156300703083	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.12439156300703083	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	45	0.12168739859383451	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	44	0.11898323418063819	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	44	0.11898323418063819	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	44	0.11898323418063819	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	44	0.11898323418063819	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	44	0.11898323418063819	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	43	0.11627906976744186	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTC	43	0.11627906976744186	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	43	0.11627906976744186	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	43	0.11627906976744186	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	42	0.11357490535424553	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	42	0.11357490535424553	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	41	0.1108707409410492	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	41	0.1108707409410492	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	41	0.1108707409410492	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	41	0.1108707409410492	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	41	0.1108707409410492	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	41	0.1108707409410492	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	38	0.10275824770146025	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	38	0.10275824770146025	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	38	0.10275824770146025	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	37	0.10005408328826393	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	37	0.10005408328826393	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	37	0.10005408328826393	No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA	37	0.10005408328826393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0027041644131963224	0.0
17	0.0	0.0	0.0	0.005408328826392645	0.0
18	0.0	0.0	0.0	0.005408328826392645	0.0
19	0.0	0.0	0.0	0.005408328826392645	0.0
20	0.0	0.0	0.0	0.005408328826392645	0.0
21	0.0	0.0	0.0	0.01352082206598161	0.0
22	0.0	0.0	0.0	0.018929150892374257	0.0
23	0.0	0.0	0.0	0.02163331530557058	0.0
24	0.0	0.0	0.0	0.02704164413196322	0.0
25	0.0	0.0	0.0	0.029745808545159545	0.0
26	0.0	0.0	0.0	0.04056246619794483	0.0
27	0.0	0.0	0.0	0.09194159004867496	0.0
28	0.0	0.0	0.0	0.27582477014602486	0.0
29	0.0	0.0	0.0	0.5949161709031909	0.0
30	0.0	0.0	0.0	1.1222282314764738	0.0
31	0.0	0.0	0.0	2.1930773391022176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACA	20	0.0018189795	37.0	37
GGTATCA	75	1.2732926E-11	29.6	1
TGTGACG	25	0.0054286225	29.599998	12
TATCGCG	25	0.0054286225	29.599998	7
ATCGCGC	25	0.0054286225	29.599998	8
GTGTGAC	25	0.0054286225	29.599998	11
GCAGCCG	70	6.162736E-9	26.428572	17
AGCCGCG	70	6.162736E-9	26.428572	19
CAGCCGC	70	6.162736E-9	26.428572	18
CAGCAGC	75	1.2845703E-8	24.666666	15
TGCCAGC	75	1.2845703E-8	24.666666	12
CGTGCCA	75	1.2845703E-8	24.666666	10
GCCGCGG	85	1.8244464E-9	23.941177	20
GTGCCAG	85	1.8244464E-9	23.941177	11
GCGGTAA	80	2.5496774E-8	23.125002	23
GCGCGCC	40	0.0018962286	23.125002	11
CGCGGTA	80	2.5496774E-8	23.125002	22
CCGCGGT	80	2.5496774E-8	23.125002	21
AACTCCG	75	3.5424455E-7	22.2	5
TAACTCC	75	3.5424455E-7	22.2	4
>>END_MODULE