FastQCFastQC Report
Fri 10 Feb 2017
ERR1632375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107232
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATAC200.00183450237.033
TGGCTAA250.005474413329.623
GGCCTAG250.005474413329.61
CCACTAT400.001919848623.12536
TCAGCGT400.001919848623.12513
TCAGTGC400.001919848623.1258
CAGCGTG555.101405E-420.18181814
GTATCAA555.101405E-420.1818181
GAAGTGA500.006994363418.56
TCAACGC609.16256E-418.54
TCTTATA1502.419256E-1017.26666637
CAACGCA650.001567336617.0769235
TTCAAGA650.001567336617.0769232
TATCAAC700.002571992315.8571422
ATCAACG700.002571992315.8571423
GCTCAGC700.002571992315.85714211
GACACAG750.004072216314.87
AACGCAG850.00933218913.0588246
CTCTTAT2152.7739588E-912.90697736
GCAGAGT1200.00709116810.7916663
Produced by FastQC (version 0.11.5)