##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632375.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107232
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75020516263802	34.0	31.0	34.0	31.0	34.0
2	32.92001454789615	34.0	31.0	34.0	31.0	34.0
3	32.92473328857058	34.0	33.0	34.0	31.0	34.0
4	36.375662115786334	37.0	37.0	37.0	35.0	37.0
5	36.29069680692331	37.0	37.0	37.0	35.0	37.0
6	36.31758243807818	37.0	37.0	37.0	35.0	37.0
7	36.30569233064757	37.0	37.0	37.0	35.0	37.0
8	36.287209042076995	37.0	37.0	37.0	35.0	37.0
9	38.070594598627274	39.0	39.0	39.0	35.0	39.0
10	37.995383840644585	39.0	38.0	39.0	35.0	39.0
11	38.11596351835273	39.0	39.0	39.0	37.0	39.0
12	38.079416592062074	39.0	38.0	39.0	37.0	39.0
13	38.102972993136376	39.0	38.0	39.0	37.0	39.0
14	39.50439234556849	40.0	39.0	41.0	37.0	41.0
15	39.531445837063565	41.0	39.0	41.0	37.0	41.0
16	39.42264435989257	41.0	39.0	41.0	37.0	41.0
17	39.47026074306177	41.0	39.0	41.0	37.0	41.0
18	39.47677931960609	41.0	39.0	41.0	37.0	41.0
19	39.53996008654133	41.0	39.0	41.0	37.0	41.0
20	39.4980975828111	41.0	39.0	41.0	37.0	41.0
21	39.48182445538645	41.0	39.0	41.0	37.0	41.0
22	39.465625932557444	41.0	39.0	41.0	37.0	41.0
23	39.42267233661594	40.0	39.0	41.0	37.0	41.0
24	39.38123880931065	41.0	39.0	41.0	37.0	41.0
25	39.31317144136079	40.0	39.0	41.0	36.0	41.0
26	39.22924127126231	40.0	39.0	41.0	36.0	41.0
27	39.16144434497165	40.0	39.0	41.0	36.0	41.0
28	39.09469188301999	40.0	39.0	41.0	36.0	41.0
29	38.973972321695015	40.0	39.0	41.0	35.0	41.0
30	38.92494777678305	40.0	39.0	41.0	35.0	41.0
31	38.859762011339896	40.0	38.0	41.0	35.0	41.0
32	38.799490823634734	40.0	38.0	41.0	35.0	41.0
33	38.65013242315727	40.0	38.0	41.0	35.0	41.0
34	38.65901037003879	40.0	38.0	41.0	35.0	41.0
35	38.63753357206804	40.0	38.0	41.0	35.0	41.0
36	38.607859594151	40.0	38.0	41.0	35.0	41.0
37	38.57591017606685	40.0	38.0	41.0	35.0	41.0
38	38.51219785138765	40.0	38.0	41.0	35.0	41.0
39	38.33099261414503	40.0	38.0	41.0	34.0	41.0
40	38.35234817964787	40.0	38.0	41.0	34.0	41.0
41	38.37073821247389	40.0	38.0	41.0	34.0	41.0
42	38.24534653834676	40.0	38.0	41.0	34.0	41.0
43	37.38827961802447	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	4.0
20	6.0
21	15.0
22	18.0
23	40.0
24	61.0
25	114.0
26	132.0
27	205.0
28	317.0
29	470.0
30	625.0
31	893.0
32	1205.0
33	1752.0
34	2621.0
35	3853.0
36	6192.0
37	11241.0
38	26797.0
39	50665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.846351835273055	17.255110414801553	12.2659280811698	27.6326096687556
2	20.17308266189197	19.613548194568786	32.57423157266487	27.639137570874368
3	20.771784541927783	20.392233661593554	28.71064607579827	30.125335720680397
4	15.998955535660997	14.711093703372127	32.425022381378696	36.86492837958818
5	16.84944792599224	34.33583258728738	32.79897791703969	16.015741569680692
6	37.519583706356315	32.50895255147717	14.33061026559236	15.640853476574158
7	31.165137272455983	28.064383766039985	20.11246642793196	20.65801253357207
8	28.673343777976722	31.528834676216057	19.242390331244405	20.555431214562816
9	27.49832139659803	13.556587585795285	17.942405252163535	41.002685765443154
10	17.974112205311847	25.46627872276932	31.81419725455088	24.74541181736795
11	38.455871381677106	19.716129513578036	19.659243509400177	22.16875559534467
12	23.680431214562816	23.542412712623097	26.864182333631753	25.912973739182334
13	32.92767084452402	17.789465831095193	22.216316025067144	27.066547299313637
14	24.84053267681289	18.88895105938526	22.470904207699192	33.79961205610266
15	27.42371680095494	25.595904207699192	19.855080572963292	27.12529841838257
16	27.48992837958818	23.922896150402863	21.378879438973442	27.208296031035513
17	26.201133989853776	24.358400477469413	22.662078484034616	26.778387048642195
18	26.066845717696207	23.114368845120858	23.866942703670546	26.951842733512382
19	27.035772903610862	23.027641002685765	23.7475753506416	26.189010743061775
20	27.451693524321097	22.935317815577438	23.05095493882423	26.562033721277228
21	27.218554162936435	23.28036407042674	22.595866905401373	26.905214861235454
22	27.690428230378995	22.641562220232768	22.544576245896746	27.123433303491495
23	26.663682482840944	22.987541032527602	22.842994628469114	27.505781856162336
24	27.093591465234258	23.171254849298716	22.732020292450013	27.00313339301701
25	27.277305282005372	22.719897045658012	22.875634139062967	27.127163533273652
26	26.813824231572664	23.013652641002686	22.754401671142944	27.418121456281707
27	26.83340793792898	22.768390032826023	22.91946433900328	27.478737690241715
28	26.36712921515965	22.808490002984183	23.114368845120858	27.710011936735302
29	25.857952849895554	23.348440763951057	23.678566099671738	27.115040286481644
30	26.194606087735007	24.00962399283796	22.773985377499255	27.021784541927786
31	26.75507311250373	23.647791703968966	22.813152790211877	26.78398239331543
32	25.60243210981796	23.309273351238435	23.543345270068635	27.544949268874962
33	25.540883318412416	22.93625037302298	24.0226797970755	27.500186511489105
34	26.387645478961502	22.47929722470904	23.628207997612652	27.5048492987168
35	26.051924798567594	22.47276932259027	24.62138167711131	26.853924201730827
36	25.768427335123846	22.767457475380485	24.22131453297523	27.242800656520444
37	25.998769024171892	21.583109519546404	24.84706057893166	27.571060877350046
38	24.857318710832587	22.203260220829605	25.82065055207401	27.1187705162638
39	25.058751119068933	21.630669949268874	26.254289764249478	27.05628916741271
40	24.14111459265891	20.991868099074903	27.751044464339003	27.11597284392719
41	23.620747538048345	21.22500746045956	28.039204715010445	27.115040286481644
42	23.654319606087736	20.79323336317517	28.466316025067144	27.08613100566995
43	22.655550581915847	20.77551477170994	28.800171590569978	27.768763055804236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	6.5
4	10.0
5	10.0
6	9.0
7	8.0
8	9.0
9	10.0
10	15.5
11	21.0
12	21.0
13	36.0
14	51.0
15	70.0
16	89.0
17	92.5
18	96.0
19	96.0
20	131.0
21	166.0
22	216.0
23	266.0
24	372.0
25	478.0
26	478.0
27	584.0
28	690.0
29	896.0
30	1102.0
31	1412.5
32	1723.0
33	1723.0
34	2039.0
35	2355.0
36	2736.5
37	3118.0
38	3593.0
39	4068.0
40	4068.0
41	4571.5
42	5075.0
43	5385.0
44	5695.0
45	6220.0
46	6745.0
47	6745.0
48	7106.0
49	7467.0
50	7972.5
51	8478.0
52	9142.5
53	9807.0
54	9807.0
55	9285.0
56	8763.0
57	8691.0
58	8619.0
59	8120.5
60	7622.0
61	7622.0
62	7147.0
63	6672.0
64	6096.5
65	5521.0
66	4817.0
67	4113.0
68	4113.0
69	3566.5
70	3020.0
71	2636.0
72	2252.0
73	1779.5
74	1307.0
75	1307.0
76	1017.0
77	727.0
78	613.0
79	499.0
80	396.0
81	293.0
82	293.0
83	216.0
84	139.0
85	116.5
86	94.0
87	67.5
88	41.0
89	41.0
90	32.0
91	23.0
92	13.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	107232.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.29196508504923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.34493122712828	75.06900925096987
2	4.1539043948102	6.753581020590868
3	1.2286196097325945	2.996307072515667
4	0.6401211412052173	2.0814682184422564
5	0.39806816487134483	1.6179871680095492
6	0.26384921590896054	1.2869292748433303
7	0.16978123458489636	0.9661295135780364
8	0.14569065400190429	0.9474783646672634
9	0.0975094928359202	0.7134064458370636
>10	0.5494946713930092	7.176962100865413
>50	0.008030193527664016	0.3907415696806923
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0018651148910772903	0.0
17	0.0	0.0	0.0	0.0018651148910772903	0.0
18	0.0	0.0	0.0	0.0027976723366159354	0.0
19	0.0	0.0	0.0	0.0027976723366159354	0.0
20	0.0	0.0	0.0	0.0027976723366159354	0.0
21	0.0	0.0	0.0	0.0037302297821545805	0.0
22	0.0	0.0	0.0	0.005595344673231871	0.0
23	0.0	0.0	0.0	0.011190689346463742	0.0
24	0.0	0.0	0.0	0.016786034019695615	0.0
25	0.0	0.0	0.0	0.022381378692927483	0.0
26	0.0	0.0	0.0	0.024246493584004776	0.0
27	0.0	0.0	0.0	0.04289764249477768	0.0
28	0.0	0.0	0.0	0.10537899134586691	0.0
29	0.0	0.0	0.0	0.21075798269173382	0.0
30	0.0	0.0	0.0	0.34784392718591467	0.0
31	0.0	0.0	0.0	0.6639809012235154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAATAC	20	0.001834502	37.0	33
TGGCTAA	25	0.0054744133	29.6	23
GGCCTAG	25	0.0054744133	29.6	1
CCACTAT	40	0.0019198486	23.125	36
TCAGCGT	40	0.0019198486	23.125	13
TCAGTGC	40	0.0019198486	23.125	8
CAGCGTG	55	5.101405E-4	20.181818	14
GTATCAA	55	5.101405E-4	20.181818	1
GAAGTGA	50	0.0069943634	18.5	6
TCAACGC	60	9.16256E-4	18.5	4
TCTTATA	150	2.419256E-10	17.266666	37
CAACGCA	65	0.0015673366	17.076923	5
TTCAAGA	65	0.0015673366	17.076923	2
TATCAAC	70	0.0025719923	15.857142	2
ATCAACG	70	0.0025719923	15.857142	3
GCTCAGC	70	0.0025719923	15.857142	11
GACACAG	75	0.0040722163	14.8	7
AACGCAG	85	0.009332189	13.058824	6
CTCTTAT	215	2.7739588E-9	12.906977	36
GCAGAGT	120	0.007091168	10.791666	3
>>END_MODULE