FastQCFastQC Report
Fri 10 Feb 2017
ERR1632374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3985838
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG57210.14353317922103206No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA53760.13487753390880414No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC48690.12215749862387784No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG47410.11894612876890631No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT42220.10592502756007645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.025.2815251
CTTATAC26800.019.60447737
TCTTATA48150.017.21287737
TATACCG803.385186E-416.18755
GTATCAA26450.016.0170142
TAGTTCG1101.4535777E-515.1363647
TCTATAC2700.015.0740753
CTATACT4500.014.84
GCTTAGG15650.014.30351451
TAGGACA16250.013.8892314
CTCTTAT77350.013.82417537
AGGACCG6300.013.8015875
CTTAGGA16500.013.790912
TATACAA5850.013.5982915
TATAGTA3150.013.50793652
TATGCCG2750.013.45454633
TATACAC9450.013.31216837
TTAGGAC17800.013.303373
TCGTATG2651.8189894E-1213.26415230
ATAGGAC5600.013.2142853
Produced by FastQC (version 0.11.5)