##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3985838
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75245205650606	34.0	31.0	34.0	31.0	34.0
2	32.91223953407037	34.0	31.0	34.0	31.0	34.0
3	32.91335899753076	34.0	33.0	34.0	31.0	34.0
4	36.370934041975616	37.0	37.0	37.0	35.0	37.0
5	36.28209149493783	37.0	37.0	37.0	35.0	37.0
6	36.31655551480015	37.0	37.0	37.0	35.0	37.0
7	36.306665750088186	37.0	37.0	37.0	35.0	37.0
8	36.28261233898618	37.0	37.0	37.0	35.0	37.0
9	38.07406071195066	39.0	38.0	39.0	37.0	39.0
10	38.00103767388439	39.0	38.0	39.0	35.0	39.0
11	38.116014248446625	39.0	38.0	39.0	37.0	39.0
12	38.08315265196428	39.0	38.0	39.0	37.0	39.0
13	38.10252348439651	39.0	38.0	39.0	37.0	39.0
14	39.50539510135635	40.0	39.0	41.0	37.0	41.0
15	39.535476604919715	40.0	39.0	41.0	37.0	41.0
16	39.417541555878586	40.0	39.0	41.0	37.0	41.0
17	39.462147232275875	40.0	39.0	41.0	37.0	41.0
18	39.467141665065164	40.0	39.0	41.0	37.0	41.0
19	39.52435322258456	41.0	39.0	41.0	37.0	41.0
20	39.505323849087695	41.0	39.0	41.0	37.0	41.0
21	39.472911091720235	41.0	39.0	41.0	37.0	41.0
22	39.459910061573	40.0	39.0	41.0	37.0	41.0
23	39.4170952256464	40.0	39.0	41.0	37.0	41.0
24	39.36221366748975	40.0	39.0	41.0	37.0	41.0
25	39.30151476301847	40.0	39.0	41.0	36.0	41.0
26	39.22113844064912	40.0	39.0	41.0	36.0	41.0
27	39.1513744412091	40.0	39.0	41.0	36.0	41.0
28	39.08217895458872	40.0	39.0	41.0	36.0	41.0
29	38.95052784383108	40.0	39.0	41.0	35.0	41.0
30	38.9103776922193	40.0	38.0	41.0	35.0	41.0
31	38.84958796619431	40.0	38.0	41.0	35.0	41.0
32	38.78406749095171	40.0	38.0	41.0	35.0	41.0
33	38.656142071002385	40.0	38.0	41.0	35.0	41.0
34	38.66340177398078	40.0	38.0	41.0	35.0	41.0
35	38.637894967131125	40.0	38.0	41.0	35.0	41.0
36	38.61565046045524	40.0	38.0	41.0	35.0	41.0
37	38.590173509309714	40.0	38.0	41.0	35.0	41.0
38	38.513977989070305	40.0	38.0	41.0	35.0	41.0
39	38.34101661934078	40.0	38.0	41.0	34.0	41.0
40	38.355486098531856	40.0	38.0	41.0	34.0	41.0
41	38.36557632297148	40.0	38.0	41.0	34.0	41.0
42	38.24851561955102	40.0	38.0	41.0	34.0	41.0
43	37.38398976576569	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	1.0
13	0.0
14	5.0
15	4.0
16	10.0
17	27.0
18	71.0
19	129.0
20	269.0
21	451.0
22	944.0
23	1594.0
24	2489.0
25	3814.0
26	5530.0
27	7981.0
28	11682.0
29	16357.0
30	22969.0
31	31986.0
32	44858.0
33	63433.0
34	94899.0
35	144751.0
36	234131.0
37	426255.0
38	1007677.0
39	1863517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.519831965072335	16.835029421667414	12.022139384490789	27.62299922876946
2	20.05814084767118	19.720645946975264	32.78505047119326	27.4361627341603
3	20.798311421588135	20.518721533589673	28.850996954718177	29.831970090104015
4	16.0188647907918	14.77930111559978	31.750186535428686	37.45164755817974
5	16.749501610451805	34.33724601953215	32.516173512320364	16.39707885769567
6	37.39745569187709	32.10835462956598	14.810762504647707	15.683427173909227
7	31.49681447163683	28.49004400078478	19.55483388938537	20.45830763819302
8	28.383466663722913	31.81346055710242	19.35592966899307	20.4471431101816
9	27.53714526280295	13.828409483777312	17.99769584212906	40.63674941129067
10	18.19343887032037	25.701320525319893	31.314719765329148	24.790520839030588
11	37.97587859817684	20.0071854400505	19.824312980106065	22.19262298166659
12	23.526721356964334	23.653620643889692	26.808666082264253	26.01099191688172
13	32.236257469571015	17.8302028331307	23.107110725523718	26.82642897177457
14	24.626665709946064	18.92437675590428	22.46902658863707	33.979930945512585
15	27.526181445407467	25.664640660257643	20.22129348959993	26.587884404734965
16	27.69387516502176	23.995631533444158	21.489082095157908	26.821411206376172
17	26.433161608675515	24.272060229241628	22.73981029836135	26.554967863721508
18	25.708445752185614	23.206035970353035	24.19290498008198	26.89261329737937
19	27.24149852552964	23.284839975934798	23.680239889328167	25.7934216092074
20	27.40051151100471	22.62866679478694	23.786942670524994	26.18387902368335
21	27.34165312288156	23.402004797987274	22.52203928007109	26.73430279906007
22	27.52748606441105	22.79560784959148	22.652927690488173	27.023978395509303
23	26.483339262659445	23.40376101587671	22.976071782144682	27.136827939319158
24	27.41476196473615	23.2648441808222	22.883669632333277	26.436724222108378
25	27.37456966389502	22.659852206737956	22.95775693844055	27.007821190926474
26	27.18482286535479	23.104978175229398	22.899776659262116	26.810422300153697
27	26.997936193091643	22.671970110175074	23.211505334637284	27.118588362096002
28	26.388578763110793	23.052943948048064	23.529556394414424	27.028920894426715
29	26.11056947121283	23.5015321746644	23.641828895203467	26.7460694589193
30	25.927947899538317	24.380017451788056	23.22535436713685	26.46668028153678
31	27.135849475066472	23.32126895272713	23.023815819910393	26.519065752296004
32	25.60656002577124	23.346959911566902	23.771538130751928	27.27494193190993
33	26.02536781474812	22.865430055110117	23.801770167277244	27.30743196286452
34	26.51592964892201	22.622645476308872	24.00805050280518	26.85337437196394
35	26.24860819732262	22.679145514694778	24.230161888165046	26.842084399817555
36	25.768583670485352	23.145421364340447	24.329965241939085	26.756029723235113
37	26.544505822865855	21.634622380538296	24.601677238262067	27.219194558333783
38	25.238908355030986	22.148516823814717	25.91577981844721	26.696795002707084
39	25.299372428081625	21.53318825300978	26.269331568417986	26.898107750490613
40	24.534614803712547	21.3070626553312	27.322585614367668	26.835736926588588
41	23.95388372532953	21.572301734290257	27.8458131012851	26.628001439095115
42	24.294740528842365	21.02704625727388	27.69201859182435	26.9861946220594
43	23.026399969090566	20.943901884622505	28.396236876661824	27.63346126962511
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	27.5
2	34.0
3	97.0
4	160.0
5	160.0
6	208.0
7	256.0
8	272.5
9	289.0
10	437.5
11	586.0
12	586.0
13	998.5
14	1411.0
15	2490.0
16	3569.0
17	3796.0
18	4023.0
19	4023.0
20	5087.5
21	6152.0
22	7674.5
23	9197.0
24	11939.0
25	14681.0
26	14681.0
27	17895.0
28	21109.0
29	31563.5
30	42018.0
31	49644.0
32	57270.0
33	57270.0
34	69731.0
35	82192.0
36	95191.0
37	108190.0
38	133376.0
39	158562.0
40	158562.0
41	180421.5
42	202281.0
43	206173.0
44	210065.0
45	232762.5
46	255460.0
47	255460.0
48	277985.0
49	300510.0
50	322858.0
51	345206.0
52	372859.5
53	400513.0
54	400513.0
55	364199.5
56	327886.0
57	314447.5
58	301009.0
59	280583.5
60	260158.0
61	260158.0
62	244262.0
63	228366.0
64	208232.0
65	188098.0
66	165748.0
67	143398.0
68	143398.0
69	124655.5
70	105913.0
71	92451.5
72	78990.0
73	64345.0
74	49700.0
75	49700.0
76	40360.0
77	31020.0
78	26258.5
79	21497.0
80	16904.0
81	12311.0
82	12311.0
83	9576.0
84	6841.0
85	5245.5
86	3650.0
87	2758.5
88	1867.0
89	1867.0
90	1453.0
91	1039.0
92	635.5
93	232.0
94	150.5
95	69.0
96	69.0
97	51.0
98	33.0
99	19.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3985838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.66248613874668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.06974569162043	43.43252071481298
2	9.074130944168871	9.375843282485933
3	2.609854316441104	4.044946873418601
4	1.2121553001205854	2.504918255619522
5	0.6860770513645144	1.7722223078115706
6	0.43072745439477667	1.3351470685348676
7	0.30803124811691684	1.113956206029874
8	0.2197735500185174	0.9083238385195843
9	0.16676562263223257	0.7753974000891456
>10	1.0061909183365938	9.713245481590937
>50	0.1026775126700324	3.7523990024357525
>100	0.09373116577629118	10.31653112445538
>500	0.01316607887790092	4.788503879650748
>1k	0.006875618951322227	5.886485502231612
>5k	9.752650994783303E-5	0.2795590623133595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5721	0.14353317922103206	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	5376	0.13487753390880414	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	4869	0.12215749862387784	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	4741	0.11894612876890631	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	4222	0.10592502756007645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.5088826991965052E-5	0.0	0.0	0.0	0.0
3	2.5088826991965052E-5	0.0	0.0	0.0	0.0
4	2.5088826991965052E-5	2.5088826991965052E-5	0.0	0.0	0.0
5	5.0177653983930104E-5	2.5088826991965052E-5	0.0	0.0	0.0
6	5.0177653983930104E-5	2.5088826991965052E-5	0.0	0.0	0.0
7	5.0177653983930104E-5	2.5088826991965052E-5	0.0	0.0	0.0
8	5.0177653983930104E-5	2.5088826991965052E-5	0.0	2.5088826991965052E-5	0.0
9	5.0177653983930104E-5	2.5088826991965052E-5	0.0	2.5088826991965052E-5	0.0
10	5.0177653983930104E-5	2.5088826991965052E-5	0.0	7.526648097589516E-5	0.0
11	5.0177653983930104E-5	2.5088826991965052E-5	0.0	1.0035530796786021E-4	0.0
12	5.0177653983930104E-5	2.5088826991965052E-5	0.0	1.0035530796786021E-4	0.0
13	5.0177653983930104E-5	2.5088826991965052E-5	0.0	1.0035530796786021E-4	0.0
14	5.0177653983930104E-5	2.5088826991965052E-5	0.0	1.0035530796786021E-4	0.0
15	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	1.2544413495982527E-4	2.5088826991965052E-5
16	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	2.2579944292768547E-4	2.5088826991965052E-5
17	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	3.2615475089554566E-4	2.5088826991965052E-5
18	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	3.7633240487947576E-4	2.5088826991965052E-5
19	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	6.021318478071613E-4	2.5088826991965052E-5
20	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	7.275759827669866E-4	2.5088826991965052E-5
21	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	8.530201177268118E-4	2.5088826991965052E-5
22	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.0012544413495982527	2.5088826991965052E-5
23	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.001982017332365239	2.5088826991965052E-5
24	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.0035877022598510026	2.5088826991965052E-5
25	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.0043403670696099544	2.5088826991965052E-5
26	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.007075049211734145	2.5088826991965052E-5
27	5.0177653983930104E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.019895439804628285	2.5088826991965052E-5
28	7.526648097589516E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.06974693903766284	2.5088826991965052E-5
29	7.526648097589516E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.1419274942935463	2.5088826991965052E-5
30	7.526648097589516E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.24015025196708947	2.5088826991965052E-5
31	7.526648097589516E-5	2.5088826991965052E-5	2.5088826991965052E-5	0.5868025745150706	2.5088826991965052E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1705	0.0	25.281525	1
CTTATAC	2680	0.0	19.604477	37
TCTTATA	4815	0.0	17.212877	37
TATACCG	80	3.385186E-4	16.1875	5
GTATCAA	2645	0.0	16.017014	2
TAGTTCG	110	1.4535777E-5	15.136364	7
TCTATAC	270	0.0	15.074075	3
CTATACT	450	0.0	14.8	4
GCTTAGG	1565	0.0	14.3035145	1
TAGGACA	1625	0.0	13.889231	4
CTCTTAT	7735	0.0	13.824175	37
AGGACCG	630	0.0	13.801587	5
CTTAGGA	1650	0.0	13.79091	2
TATACAA	585	0.0	13.598291	5
TATAGTA	315	0.0	13.5079365	2
TATGCCG	275	0.0	13.454546	33
TATACAC	945	0.0	13.312168	37
TTAGGAC	1780	0.0	13.30337	3
TCGTATG	265	1.8189894E-12	13.264152	30
ATAGGAC	560	0.0	13.214285	3
>>END_MODULE