FastQCFastQC Report
Fri 10 Feb 2017
ERR1632373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984493
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4950.025.4141431
GTCCTAT1457.2759576E-1219.137931
CTTATAC8850.018.60451937
TCCATTA1103.849891E-818.58
ACCGCGT609.235529E-418.58
GTATCAA7400.017.02
TCTTATA15300.016.44444537
GTTTTCG1251.656881E-716.2828
GTGCATA700.0025921415.8571421
CGGAATG700.0025921415.8571424
ACCGTGT855.363181E-415.2352938
ATAGTAC1101.4513684E-515.1363643
TTTATAG1353.9701627E-715.0740733
GCGTTAT750.004103850614.7999991
ATACCGC1508.095594E-814.79999927
TAGTACA750.004103850614.7999994
CGCGGTT1152.2090955E-514.47826110
TTTCGGA1152.2090955E-514.47826130
TATCCTT1152.2090955E-514.4782615
CTAGACT908.2725286E-414.3888884