##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632373.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	984493
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.758844400112544	34.0	31.0	34.0	31.0	34.0
2	32.92302027541079	34.0	31.0	34.0	31.0	34.0
3	32.931866453088034	34.0	33.0	34.0	31.0	34.0
4	36.38127239096672	37.0	37.0	37.0	35.0	37.0
5	36.2986461051526	37.0	37.0	37.0	35.0	37.0
6	36.33166208393559	37.0	37.0	37.0	35.0	37.0
7	36.318612727566375	37.0	37.0	37.0	35.0	37.0
8	36.29723014790354	37.0	37.0	37.0	35.0	37.0
9	38.09022613670184	39.0	39.0	39.0	37.0	39.0
10	38.0337645874577	39.0	38.0	39.0	35.0	39.0
11	38.1337896765137	39.0	38.0	39.0	37.0	39.0
12	38.10275847568241	39.0	38.0	39.0	37.0	39.0
13	38.12096073816675	39.0	38.0	39.0	37.0	39.0
14	39.52709160958991	40.0	39.0	41.0	37.0	41.0
15	39.55739451677157	41.0	39.0	41.0	37.0	41.0
16	39.44769338126325	41.0	39.0	41.0	37.0	41.0
17	39.49039861126489	41.0	39.0	41.0	37.0	41.0
18	39.49486182227807	41.0	39.0	41.0	37.0	41.0
19	39.549689027753374	41.0	39.0	41.0	37.0	41.0
20	39.52991031932172	41.0	39.0	41.0	37.0	41.0
21	39.49769881553246	41.0	39.0	41.0	37.0	41.0
22	39.47949960030188	41.0	39.0	41.0	37.0	41.0
23	39.435269727666935	41.0	39.0	41.0	37.0	41.0
24	39.380096151013774	41.0	39.0	41.0	37.0	41.0
25	39.32338472696098	40.0	39.0	41.0	36.0	41.0
26	39.24711805975258	40.0	39.0	41.0	36.0	41.0
27	39.18680782900437	40.0	39.0	41.0	36.0	41.0
28	39.11692718993431	40.0	39.0	41.0	36.0	41.0
29	38.99354185352257	40.0	39.0	41.0	35.0	41.0
30	38.94818855999992	40.0	39.0	41.0	35.0	41.0
31	38.88226427206694	40.0	38.0	41.0	35.0	41.0
32	38.821908332512265	40.0	38.0	41.0	35.0	41.0
33	38.70714977150676	40.0	38.0	41.0	35.0	41.0
34	38.70395929681572	40.0	38.0	41.0	35.0	41.0
35	38.67455126648945	40.0	38.0	41.0	35.0	41.0
36	38.656368303278946	40.0	38.0	41.0	35.0	41.0
37	38.635548449811225	40.0	38.0	41.0	35.0	41.0
38	38.56704110643753	40.0	38.0	41.0	35.0	41.0
39	38.416196966357305	40.0	38.0	41.0	34.0	41.0
40	38.41457582735479	40.0	38.0	41.0	34.0	41.0
41	38.423041098311515	40.0	38.0	41.0	34.0	41.0
42	38.31343036466486	40.0	38.0	41.0	34.0	41.0
43	37.4719505369769	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	2.0
14	2.0
15	1.0
16	1.0
17	10.0
18	11.0
19	29.0
20	69.0
21	98.0
22	179.0
23	381.0
24	601.0
25	950.0
26	1387.0
27	1957.0
28	2756.0
29	3890.0
30	5477.0
31	7724.0
32	10771.0
33	15080.0
34	22861.0
35	34881.0
36	56462.0
37	103452.0
38	246143.0
39	469314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66632672858009	17.704950670040315	12.363622697164937	27.265099904214658
2	19.571495175689417	19.767636742973288	33.82675143449471	26.83411664684259
3	20.431227037673196	20.985217771990254	29.143122399041943	29.440432791294608
4	15.674057611379663	15.123317281077671	32.88159489199009	36.321030215552575
5	16.39077169670074	34.411519431829376	33.097340458489796	16.10036841298008
6	36.40747064732812	33.02776149754239	15.142921280293512	15.421846574835982
7	30.984374698448846	28.82478595581685	20.182266405144578	20.008572940589726
8	27.88714597259706	32.28423157909705	19.607655920356976	20.220966527948903
9	27.38495855227005	13.86571565262526	18.675500993912603	40.07382480119209
10	17.852234601972793	25.790635382882353	31.98956214010663	24.36756787503822
11	37.23469846916128	20.54610850458053	20.303953405458444	21.915239620799742
12	22.96258073952786	24.139938018858437	27.71914071506857	25.178340526545135
13	31.8542640729797	18.403178082525727	23.381374981843447	26.361182862651134
14	24.157104215062983	19.587036169886428	23.327235439967577	32.92862417508301
15	26.932339793172726	26.03756451290156	20.798319541124215	26.231776152801494
16	26.733861998003032	24.773868376920912	22.33108818447668	26.161181440599375
17	25.51953137300113	24.893117574223485	23.680818451730993	25.906532601044397
18	25.19835082626286	23.721854802421145	24.940146857316407	26.13964751399959
19	26.339141060423994	23.912105012427716	24.4912863778615	25.257467549286787
20	26.7170005271749	23.368576516034143	24.259999817164772	25.654423139626182
21	26.822435507413463	23.854715066536787	23.301943233725382	26.02090619232437
22	27.01745974831715	23.604738682753457	23.254202924754164	26.123598644175228
23	26.099830064815087	23.821500000507875	23.646993934949258	26.431675999727776
24	26.356713557130423	23.994990314811787	23.64841598670585	25.999880141351944
25	26.410751523880823	23.339221304773115	23.734145392603097	26.515881778742965
26	26.020703042073436	23.910581385545658	23.787066032973318	26.281649539407592
27	26.360878137274717	23.461416180714338	23.59427644482998	26.583429237180965
28	25.742590348534726	23.84364337786048	24.05837319310549	26.3553930804993
29	25.432481490472764	24.035315639623644	24.407893199850076	26.12430967005352
30	25.480221799443974	24.626584444988435	24.076555140564736	25.81663861500285
31	26.153563306189078	24.02739277983693	23.644556131937964	26.17448778203603
32	24.93770905430511	23.900119147622174	24.38158524235317	26.780586555719545
33	25.14746168840205	23.534651846178694	24.599971762115118	26.717914703304135
34	25.881646695304077	23.08822916973508	24.75395965232866	26.276164482632176
35	25.456250069832898	23.091479573750142	25.268437662837623	26.183832693579333
36	25.019477030309	23.201891735136766	25.298808625353352	26.479822609200877
37	25.38220180336478	22.039669149501318	25.613894664563386	26.964234382570524
38	24.38412462048994	22.192641288460152	26.85504112268955	26.568192968360364
39	24.309162177892578	21.78979434084346	27.362205724164625	26.538837757099337
40	23.575586621743376	21.6403773312761	28.39319324769196	26.390842799288567
41	22.96329176540615	21.67958532970778	29.095585240321668	26.2615376645644
42	23.055725129584467	21.572118846959807	28.950028085522195	26.42212793793354
43	22.03723134649002	21.344692141030965	29.48796995001488	27.130106562464128
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	22.5
2	27.0
3	68.5
4	110.0
5	110.0
6	129.0
7	148.0
8	154.0
9	160.0
10	246.5
11	333.0
12	333.0
13	564.0
14	795.0
15	1238.5
16	1682.0
17	1806.0
18	1930.0
19	1930.0
20	2461.5
21	2993.0
22	3448.0
23	3903.0
24	5097.5
25	6292.0
26	6292.0
27	7648.5
28	9005.0
29	11798.0
30	14591.0
31	17421.0
32	20251.0
33	20251.0
34	23741.5
35	27232.0
36	31002.5
37	34773.0
38	39168.5
39	43564.0
40	43564.0
41	47753.0
42	51942.0
43	53721.0
44	55500.0
45	59037.0
46	62574.0
47	62574.0
48	65608.5
49	68643.0
50	72928.5
51	77214.0
52	81721.0
53	86228.0
54	86228.0
55	82394.0
56	78560.0
57	76113.5
58	73667.0
59	68410.5
60	63154.0
61	63154.0
62	58987.0
63	54820.0
64	49308.0
65	43796.0
66	38113.0
67	32430.0
68	32430.0
69	27815.0
70	23200.0
71	20360.5
72	17521.0
73	14175.5
74	10830.0
75	10830.0
76	8732.0
77	6634.0
78	5501.5
79	4369.0
80	3468.5
81	2568.0
82	2568.0
83	1993.5
84	1419.0
85	1156.5
86	894.0
87	664.0
88	434.0
89	434.0
90	320.0
91	206.0
92	131.0
93	56.0
94	38.5
95	21.0
96	21.0
97	13.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	984493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.18513356886504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.28438552474817	62.46337271712608
2	5.567101041272562	7.703212582636199
3	1.5207736441206978	3.156447830895003
4	0.6939905350818898	1.9205531146067465
5	0.39062138803906576	1.3512596453169115
6	0.2670387943217244	1.1085068791930306
7	0.19621997128904226	0.9502853445757877
8	0.13143579802648878	0.7274722593754396
9	0.10606746380214709	0.6604462485416075
>10	0.6992113682149237	9.471352621251453
>50	0.0928902406998085	4.467789922503161
>100	0.049379815935278036	5.673586759674839
>500	8.844144482901126E-4	0.34571407430379825
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0157512547067374E-4	0.0
5	0.0	0.0	0.0	1.0157512547067374E-4	0.0
6	0.0	0.0	0.0	1.0157512547067374E-4	0.0
7	0.0	0.0	0.0	1.0157512547067374E-4	0.0
8	0.0	0.0	0.0	1.0157512547067374E-4	0.0
9	0.0	0.0	0.0	2.0315025094134749E-4	0.0
10	0.0	0.0	0.0	2.0315025094134749E-4	0.0
11	0.0	0.0	0.0	2.0315025094134749E-4	0.0
12	0.0	0.0	0.0	2.0315025094134749E-4	0.0
13	0.0	0.0	0.0	2.0315025094134749E-4	0.0
14	0.0	0.0	0.0	2.0315025094134749E-4	0.0
15	0.0	0.0	0.0	2.0315025094134749E-4	0.0
16	0.0	1.0157512547067374E-4	0.0	5.078756273533687E-4	0.0
17	0.0	1.0157512547067374E-4	0.0	9.141761292360636E-4	0.0
18	0.0	1.0157512547067374E-4	0.0	0.0010157512547067373	0.0
19	0.0	1.0157512547067374E-4	0.0	0.0013204766311187585	0.0
20	0.0	1.0157512547067374E-4	0.0	0.001523626882060106	0.0
21	0.0	1.0157512547067374E-4	0.0	0.0020315025094134746	0.0
22	0.0	1.0157512547067374E-4	0.0	0.002742528387708191	0.0
23	0.0	2.0315025094134749E-4	0.0	0.0036567045169442545	0.0
24	0.0	2.0315025094134749E-4	0.0	0.005789782151828403	0.0
25	0.0	2.0315025094134749E-4	0.0	0.00843073541406592	0.0
26	0.0	2.0315025094134749E-4	0.0	0.012087439931010175	0.0
27	0.0	2.0315025094134749E-4	0.0	0.02986308688837808	0.0
28	0.0	2.0315025094134749E-4	0.0	0.10116882496879104	0.0
29	0.0	2.0315025094134749E-4	0.0	0.20640065495640902	0.0
30	0.0	2.0315025094134749E-4	0.0	0.34444125047105467	0.0
31	0.0	2.0315025094134749E-4	0.0	0.8301735004718165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	495	0.0	25.414143	1
GTCCTAT	145	7.2759576E-12	19.13793	1
CTTATAC	885	0.0	18.604519	37
TCCATTA	110	3.849891E-8	18.5	8
ACCGCGT	60	9.235529E-4	18.5	8
GTATCAA	740	0.0	17.0	2
TCTTATA	1530	0.0	16.444445	37
GTTTTCG	125	1.656881E-7	16.28	28
GTGCATA	70	0.00259214	15.857142	1
CGGAATG	70	0.00259214	15.857142	4
ACCGTGT	85	5.363181E-4	15.235293	8
ATAGTAC	110	1.4513684E-5	15.136364	3
TTTATAG	135	3.9701627E-7	15.074073	3
GCGTTAT	75	0.0041038506	14.799999	1
ATACCGC	150	8.095594E-8	14.799999	27
TAGTACA	75	0.0041038506	14.799999	4
CGCGGTT	115	2.2090955E-5	14.478261	10
TTTCGGA	115	2.2090955E-5	14.478261	30
TATCCTT	115	2.2090955E-5	14.478261	5
CTAGACT	90	8.2725286E-4	14.388888	4
>>END_MODULE