FastQCFastQC Report
Fri 10 Feb 2017
ERR1632372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207248
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2460.11869837103373736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.10470547363545124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG250.0054860829.59999822
CAGTCGG250.0054860829.59999810
AGTCGGT250.0054860829.59999811
TATGGGC358.844779E-426.428574
ATAACGC358.844779E-426.428573
GGACAGT358.844779E-426.428576
CTCTATG451.3181046E-424.6666661
TCTCGTA400.001925877923.12528
GCCTCGC400.001925877923.12511
ATGCCGT400.001925877923.12534
GCCGTCT400.001925877923.12536
TGCCGTC400.001925877923.12535
TCTATGG400.001925877923.1252
GGTATCA2500.022.9399991
GATACCA450.003815390220.5555556
CGTATGC450.003815390220.55555531
GACGCGT450.003815390220.55555516
AGAGCTA450.003815390220.5555555
ACGCGTA450.003815390220.55555517
GAGACGC609.202026E-418.514
Produced by FastQC (version 0.11.5)