##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632372.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207248
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.367622365475178	31.0	31.0	34.0	30.0	34.0
2	31.535927970354358	31.0	31.0	34.0	30.0	34.0
3	31.533322396356056	31.0	31.0	34.0	30.0	34.0
4	35.42151432100672	37.0	35.0	37.0	33.0	37.0
5	34.61796977534162	35.0	35.0	37.0	30.0	37.0
6	34.57945553153709	35.0	35.0	37.0	30.0	37.0
7	34.59814811240639	35.0	35.0	37.0	31.0	37.0
8	34.52231143364472	35.0	35.0	37.0	30.0	37.0
9	36.122442677372035	37.0	35.0	39.0	32.0	39.0
10	35.819149038832705	37.0	35.0	39.0	30.0	39.0
11	36.014340307264725	37.0	35.0	39.0	32.0	39.0
12	35.92120551223655	37.0	35.0	39.0	31.0	39.0
13	36.035170423840036	37.0	35.0	39.0	32.0	39.0
14	37.00419304408245	38.0	36.0	40.0	32.0	41.0
15	36.966084111788774	38.0	36.0	40.0	32.0	41.0
16	36.81285706014051	38.0	36.0	40.0	31.0	41.0
17	36.824693121284646	38.0	36.0	40.0	31.0	41.0
18	36.83388018219718	38.0	36.0	40.0	31.0	41.0
19	36.89645738438972	38.0	36.0	40.0	31.0	41.0
20	36.87963695668957	38.0	36.0	40.0	31.0	41.0
21	36.82816239481201	38.0	36.0	40.0	31.0	41.0
22	36.77845383308886	38.0	36.0	40.0	31.0	41.0
23	36.703746236393116	38.0	36.0	40.0	31.0	41.0
24	36.59513722689724	38.0	35.0	40.0	31.0	41.0
25	36.48964525592527	38.0	35.0	40.0	30.0	41.0
26	36.317339612445	38.0	35.0	40.0	30.0	41.0
27	36.20861479966031	38.0	35.0	40.0	30.0	41.0
28	36.09514687717131	38.0	35.0	40.0	30.0	41.0
29	35.91322956071952	38.0	34.0	40.0	29.0	41.0
30	35.80608256774492	38.0	34.0	40.0	29.0	41.0
31	35.69121053037906	38.0	34.0	40.0	28.0	41.0
32	35.573810121207444	38.0	34.0	40.0	28.0	41.0
33	35.39473963560565	38.0	34.0	40.0	27.0	41.0
34	35.38179379294372	38.0	34.0	40.0	27.0	41.0
35	35.31765807148923	38.0	34.0	40.0	27.0	41.0
36	35.27045375588667	38.0	34.0	40.0	27.0	41.0
37	35.22608662086003	38.0	34.0	40.0	27.0	41.0
38	35.12976723538949	38.0	33.0	40.0	27.0	41.0
39	34.93885103836949	38.0	33.0	40.0	26.0	41.0
40	34.92042866517409	37.0	33.0	40.0	26.0	40.0
41	34.89953582181734	37.0	33.0	40.0	26.0	40.0
42	34.79449741372655	37.0	33.0	40.0	26.0	40.0
43	33.816200880104994	36.0	31.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	4.0
16	10.0
17	17.0
18	36.0
19	58.0
20	108.0
21	184.0
22	282.0
23	440.0
24	712.0
25	1088.0
26	1577.0
27	2165.0
28	3117.0
29	4325.0
30	5831.0
31	7476.0
32	10562.0
33	13700.0
34	18169.0
35	24238.0
36	31369.0
37	36319.0
38	34100.0
39	11356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35071026017139	18.50295298386474	12.953080367482436	26.193256388481434
2	21.259553771327106	20.609125299158496	31.896568362541494	26.2347525669729
3	21.440978923801435	21.2286728943102	29.079170848452097	28.251177333436267
4	16.906797653053346	15.769030340461669	31.788485292982323	35.53568671350266
5	16.482185594070874	34.50503744306339	32.938797961862115	16.07397900100363
6	36.90940322705165	32.690303404616685	14.514012197946421	15.886281170385239
7	30.7105496796109	28.88085771635914	19.854956380761212	20.55363622326874
8	28.469756041071566	31.65338145603335	19.127325716050336	20.749536786844747
9	27.39471550992048	13.362734501659848	18.543001621246045	40.69954836717363
10	18.58787539566124	25.29385084536401	31.348432795491394	24.769840963483365
11	38.008569443372195	20.312379371574153	19.27690496410098	22.402146220952677
12	23.14955994750251	24.63135953061067	26.954663012429553	25.26441750945727
13	33.02372037365861	17.94709719756041	22.65643094263877	26.372751486142203
14	24.651625106153013	19.8153902570833	22.742800895545436	32.79018374121825
15	27.97759206361461	25.678414266965184	20.233729637921716	26.11026403149849
16	27.34308654365784	24.12713271056898	21.97705164826681	26.552729097506365
17	25.857909364625954	24.915560101906895	22.984057747240023	26.24247278622713
18	25.380703311974063	22.89093260248591	24.81857484752567	26.90978923801436
19	26.762140044777272	23.531710800586737	23.948602640314984	25.757546514321007
20	27.349841735505287	22.62217246969814	23.966938161043775	26.0610476337528
21	27.997857639156955	23.061742453485678	22.751968655909828	26.18843125144754
22	27.9110051725469	23.18285339303636	22.7220528062997	26.184088628117035
23	26.797363545124682	23.22048946190072	23.180440824519415	26.801706168455187
24	26.64681926966726	23.59347255462055	23.245580174476956	26.51412800123524
25	26.804118736972132	23.15390257083301	23.08876322087547	26.953215471319385
26	26.738496873311203	23.59395506832394	23.087798193468696	26.57974986489616
27	27.072878869759904	22.922295993206205	23.01300856944337	26.991816567590522
28	26.09482359299004	23.233517331892227	23.841967111865976	26.829691963251754
29	25.90616073496487	23.14376978306184	24.311935458967035	26.638134023006256
30	25.946691886049567	23.871400447772718	23.908071489230295	26.273836176947423
31	26.75827993515016	23.64317146606964	23.20167142746854	26.396877171311665
32	25.4902339226434	23.408669806222495	24.054273141357214	27.046823129776886
33	25.422682004168916	23.340152860341234	24.29359993823825	26.9435651972516
34	26.302304485447387	22.798772485138578	24.13388790241643	26.765035126997606
35	25.869489693507298	23.056917316451788	24.634254612831004	26.439338377209914
36	25.52738747780437	23.493592218018993	24.376592295221183	26.602428008955453
37	26.15224272369335	22.24725932216475	24.686366092797034	26.914131861344863
38	25.165984713965877	22.3654751794951	25.75609897321084	26.712441133328184
39	25.145719138423534	21.803346715046708	26.360688643557477	26.69024550297228
40	24.23425075272138	21.58380298000463	27.382170153632362	26.799776113641627
41	23.594437582027332	21.65038987107234	28.182660387555007	26.572512159345322
42	24.01615455878947	21.209372346174632	28.039836331351808	26.73463676368409
43	22.6284451478422	21.185246661005174	28.91801127152011	27.26829691963252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	3.0
2	2.0
3	12.0
4	22.0
5	22.0
6	28.0
7	34.0
8	42.0
9	50.0
10	58.5
11	67.0
12	67.0
13	129.5
14	192.0
15	313.0
16	434.0
17	405.5
18	377.0
19	377.0
20	478.5
21	580.0
22	530.0
23	480.0
24	660.5
25	841.0
26	841.0
27	1074.5
28	1308.0
29	1766.5
30	2225.0
31	2735.0
32	3245.0
33	3245.0
34	3903.0
35	4561.0
36	5362.5
37	6164.0
38	7053.5
39	7943.0
40	7943.0
41	8962.0
42	9981.0
43	10713.0
44	11445.0
45	12508.5
46	13572.0
47	13572.0
48	14534.5
49	15497.0
50	16347.5
51	17198.0
52	18116.5
53	19035.0
54	19035.0
55	18429.5
56	17824.0
57	17070.0
58	16316.0
59	15294.5
60	14273.0
61	14273.0
62	13474.5
63	12676.0
64	11284.5
65	9893.0
66	8536.0
67	7179.0
68	7179.0
69	6088.5
70	4998.0
71	4294.0
72	3590.0
73	2872.0
74	2154.0
75	2154.0
76	1716.5
77	1279.0
78	1081.5
79	884.0
80	661.0
81	438.0
82	438.0
83	346.5
84	255.0
85	187.5
86	120.0
87	95.0
88	70.0
89	70.0
90	51.0
91	32.0
92	20.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	207248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.89819163750968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93269768473992	59.89505664648905
2	7.225421337170945	9.956369279003106
3	2.07275866219517	4.284279705786932
4	1.001678981105143	2.760554815977904
5	0.6472634979027061	2.2297642259232746
6	0.4042352048493401	1.6710644766202691
7	0.2499989818112568	1.2057134430609988
8	0.22209147969206972	1.224136106310585
9	0.15687603114858154	0.972762737166622
>10	1.0396334733134243	13.22946486013864
>50	0.04044023560264065	1.807670539616739
>100	0.006904430468743526	0.7631631639058637
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	246	0.11869837103373736	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	217	0.10470547363545124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	4.8251370338917623E-4	0.0
16	0.0	0.0	0.0	0.0028950822203350577	0.0
17	0.0	0.0	0.0	0.004825137033891763	0.0
18	0.0	0.0	0.0	0.005307650737280939	0.0
19	0.0	0.0	0.0	0.005307650737280939	0.0
20	0.0	0.0	0.0	0.005790164440670115	0.0
21	0.0	0.0	0.0	0.00772021925422682	0.0
22	0.0	0.0	0.0	0.010615301474561878	0.0
23	0.0	0.0	0.0	0.01688797961862117	0.0
24	0.0	4.8251370338917623E-4	0.0	0.024125685169458812	0.0
25	0.0	4.8251370338917623E-4	0.0	0.02943333590673975	0.0
26	0.0	4.8251370338917623E-4	0.0	0.032810931830463984	0.0
27	0.0	4.8251370338917623E-4	0.0	0.048251370338917625	0.0
28	0.0	4.8251370338917623E-4	0.0	0.10808306955917547	0.0
29	0.0	4.8251370338917623E-4	0.0	0.19011039913533545	0.0
30	0.0	4.8251370338917623E-4	0.0	0.3006060372114568	0.0
31	0.0	4.8251370338917623E-4	0.0	0.602177101829692	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCGG	25	0.00548608	29.599998	22
CAGTCGG	25	0.00548608	29.599998	10
AGTCGGT	25	0.00548608	29.599998	11
TATGGGC	35	8.844779E-4	26.42857	4
ATAACGC	35	8.844779E-4	26.42857	3
GGACAGT	35	8.844779E-4	26.42857	6
CTCTATG	45	1.3181046E-4	24.666666	1
TCTCGTA	40	0.0019258779	23.125	28
GCCTCGC	40	0.0019258779	23.125	11
ATGCCGT	40	0.0019258779	23.125	34
GCCGTCT	40	0.0019258779	23.125	36
TGCCGTC	40	0.0019258779	23.125	35
TCTATGG	40	0.0019258779	23.125	2
GGTATCA	250	0.0	22.939999	1
GATACCA	45	0.0038153902	20.555555	6
CGTATGC	45	0.0038153902	20.555555	31
GACGCGT	45	0.0038153902	20.555555	16
AGAGCTA	45	0.0038153902	20.555555	5
ACGCGTA	45	0.0038153902	20.555555	17
GAGACGC	60	9.202026E-4	18.5	14
>>END_MODULE