FastQCFastQC Report
Fri 10 Feb 2017
ERR1632371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59957
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA730.12175392364527911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGGC200.001828056937.012
GTATCAA303.5592922E-430.8333321
TCATGAC250.005455429.63
CTCCAGA400.001910032123.1251
TATCAAC450.003784295620.5555572
TCAACGC450.003784295620.5555574
TCTTATA555.06538E-420.18181637
CAACGCA500.00695927918.55
CTCTTAT1059.051342E-615.85714237
TGCAGAG800.00620727913.8752
TCTCTTA1451.8804087E-411.48275936
CCTGTCT2757.075869E-1011.43636437
GTCTCTT2258.8083107E-710.68888937
TGTCTCT3351.8492756E-78.8358237
CTGTCTC4301.0137974E-67.313953436
Produced by FastQC (version 0.11.5)