##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632371.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59957
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76825057958203	34.0	31.0	34.0	31.0	34.0
2	32.92646396584219	34.0	31.0	34.0	31.0	34.0
3	32.92397885151025	34.0	33.0	34.0	31.0	34.0
4	36.385226078689726	37.0	37.0	37.0	35.0	37.0
5	36.294978067615126	37.0	37.0	37.0	35.0	37.0
6	36.33298864186	37.0	37.0	37.0	35.0	37.0
7	36.315709591874175	37.0	37.0	37.0	35.0	37.0
8	36.29657921510416	37.0	37.0	37.0	35.0	37.0
9	38.09988825324816	39.0	39.0	39.0	37.0	39.0
10	38.006738162349684	39.0	38.0	39.0	35.0	39.0
11	38.14485381189853	39.0	39.0	39.0	37.0	39.0
12	38.09508481078106	39.0	38.0	39.0	37.0	39.0
13	38.12687425988625	39.0	39.0	39.0	37.0	39.0
14	39.5235418716747	41.0	39.0	41.0	37.0	41.0
15	39.57089247293894	41.0	39.0	41.0	37.0	41.0
16	39.46565038277432	41.0	39.0	41.0	37.0	41.0
17	39.4918358156679	41.0	39.0	41.0	37.0	41.0
18	39.501726237136616	41.0	39.0	41.0	37.0	41.0
19	39.547625798488916	41.0	39.0	41.0	37.0	41.0
20	39.526393915639545	41.0	39.0	41.0	37.0	41.0
21	39.4939039645079	41.0	39.0	41.0	37.0	41.0
22	39.482779325183046	41.0	39.0	41.0	37.0	41.0
23	39.450539553346566	40.0	39.0	41.0	37.0	41.0
24	39.400720516370065	41.0	39.0	41.0	37.0	41.0
25	39.34558099971646	40.0	39.0	41.0	37.0	41.0
26	39.25676734993412	40.0	39.0	41.0	36.0	41.0
27	39.18424871157663	40.0	39.0	41.0	36.0	41.0
28	39.11710058875528	40.0	39.0	41.0	36.0	41.0
29	38.978951581967074	40.0	39.0	41.0	35.0	41.0
30	38.96539186416932	40.0	39.0	41.0	35.0	41.0
31	38.8808479410244	40.0	38.0	41.0	35.0	41.0
32	38.80872958953917	40.0	38.0	41.0	35.0	41.0
33	38.664909852060646	40.0	38.0	41.0	35.0	41.0
34	38.66129059159064	40.0	38.0	41.0	35.0	41.0
35	38.63150257684674	40.0	38.0	41.0	35.0	41.0
36	38.59144053238154	40.0	38.0	41.0	35.0	41.0
37	38.586386910619275	40.0	38.0	41.0	35.0	41.0
38	38.496639258135	40.0	38.0	41.0	34.0	41.0
39	38.297246359891254	40.0	38.0	41.0	34.0	41.0
40	38.30463498840836	40.0	38.0	41.0	34.0	41.0
41	38.319178744767086	40.0	38.0	41.0	34.0	41.0
42	38.18601664526244	40.0	37.0	41.0	34.0	41.0
43	37.28899044315092	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	5.0
22	17.0
23	20.0
24	38.0
25	50.0
26	89.0
27	113.0
28	139.0
29	237.0
30	347.0
31	486.0
32	650.0
33	939.0
34	1478.0
35	2177.0
36	3487.0
37	6504.0
38	15261.0
39	27916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33105392197742	17.255699918274765	11.693380255850027	27.719865903897794
2	20.35458745434228	19.497306402922092	32.256450456160245	27.89165568657538
3	20.87329252631052	20.154444018213052	27.970045199059324	31.0022182564171
4	15.908067448337976	14.690528211885184	31.505912570675648	37.89549176910119
5	17.118935236919793	33.98101973080708	32.91859165735443	15.981453374918692
6	38.7461013726504	31.97958536951482	14.226862584852478	15.047450672982304
7	31.922878062611538	27.578097636639594	19.769167903664293	20.72985639708458
8	29.25429891422186	31.255733275514118	18.765114999082677	20.724852811181346
9	27.568090464833126	13.267841953399936	17.227346264823122	41.93672131694381
10	18.356488816985504	24.702703604249713	31.53426622412729	25.40654135463749
11	39.68010407458679	19.35387027369615	18.84684023550211	22.119185416214954
12	23.740347248861685	22.969795019764163	26.92429574528412	26.36556198609003
13	33.14041729906433	16.880430975532466	22.374368297279716	27.604783428123486
14	24.312423903797722	18.596660940340577	22.10250679653752	34.98840835932418
15	27.896659272478608	24.932868555798322	19.572360191470555	27.598111980252515
16	28.392014276898443	23.251663692312825	21.161832646730154	27.194489384058574
17	27.232850209316677	23.65695415047451	22.007438664376135	27.10275697583268
18	26.807545407542072	22.174224861150492	23.62026118718415	27.397968544123287
19	27.776573210801075	22.834698200376938	22.62121186850576	26.767516720316227
20	28.03675967776907	21.81730240005337	22.844705372183398	27.301232549994165
21	28.23523525193055	22.596193938989607	22.065813833247162	27.10275697583268
22	28.198542288640194	22.447754223860432	22.052470937505213	27.301232549994165
23	27.10609269976817	22.307653818569975	22.044131627666495	28.54212185399536
24	27.279550344413494	23.036509498473905	22.33433961005387	27.349600547058728
25	28.076788364994915	22.179228447053724	22.052470937505213	27.691512250446156
26	27.421318611671698	22.983137915506113	21.942392047634137	27.65315142518805
27	27.668162182897742	22.457761395666896	22.097503210634287	27.776573210801075
28	27.0193638774455	22.564504561602483	22.509465116666945	27.906666444285072
29	26.50566239138049	22.597861800957354	23.430124922861385	27.46635088480077
30	26.547358940574078	23.670297046216454	22.39938622679587	27.3829577864136
31	27.3179111696716	22.93143419450606	22.352686091699052	27.397968544123287
32	26.187100755541472	22.70460496689294	23.14658838834498	27.961705889220607
33	26.132061310605938	22.417732708441047	23.253331554280567	28.196874426672448
34	26.99934953383258	22.035792317827777	23.590239671764763	27.37461847657488
35	26.61907700518705	22.14920693163434	23.77036876428107	27.461347298897543
36	26.190436479476958	22.75130510198976	23.473489334022716	27.58476908451057
37	26.447287222509463	21.32695098153677	24.280734526410594	27.94502726954317
38	25.448237903831078	21.54544089931117	25.158029921443703	27.848291275414045
39	25.635038444218356	20.95501776272996	25.953600080057377	27.456343712994315
40	25.123004820121086	20.76988508431042	26.87425988625181	27.232850209316677
41	24.078923228313627	20.92833197124606	27.37628633854262	27.616458461897693
42	24.285738112313826	20.71818136331037	27.724869489801023	27.271211034574776
43	22.839701786280166	20.4129626232133	27.913337892156044	28.833997698350483
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	1.0
4	0.0
5	0.0
6	2.0
7	4.0
8	4.0
9	4.0
10	4.5
11	5.0
12	5.0
13	15.0
14	25.0
15	37.0
16	49.0
17	41.0
18	33.0
19	33.0
20	48.0
21	63.0
22	82.0
23	101.0
24	129.0
25	157.0
26	157.0
27	197.0
28	237.0
29	377.5
30	518.0
31	593.0
32	668.0
33	668.0
34	873.0
35	1078.0
36	1276.5
37	1475.0
38	1777.5
39	2080.0
40	2080.0
41	2360.0
42	2640.0
43	2795.5
44	2951.0
45	3314.0
46	3677.0
47	3677.0
48	3915.5
49	4154.0
50	4415.0
51	4676.0
52	5136.0
53	5596.0
54	5596.0
55	5389.0
56	5182.0
57	5005.5
58	4829.0
59	4758.0
60	4687.0
61	4687.0
62	4419.0
63	4151.0
64	3742.5
65	3334.0
66	2915.0
67	2496.0
68	2496.0
69	2159.0
70	1822.0
71	1621.5
72	1421.0
73	1113.0
74	805.0
75	805.0
76	614.0
77	423.0
78	352.0
79	281.0
80	220.5
81	160.0
82	160.0
83	123.0
84	86.0
85	67.5
86	49.0
87	36.0
88	23.0
89	23.0
90	17.5
91	12.0
92	6.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	59957.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.27896659272479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.35384740128112	75.98779125039611
2	3.9892970080272443	6.564704705038611
3	1.3540906510986783	3.342395383358073
4	0.7033973891186248	2.314992411228047
5	0.42974134436065836	1.7679336858081625
6	0.2736560447579664	1.350968193872275
7	0.1925727722370875	1.1091282085494603
8	0.15811238141571393	1.0407458678719748
9	0.10540825427714264	0.7805594009039812
>10	0.4378496716127463	5.619026969328019
>50	0.0020270818130219736	0.12175392364527911
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	73	0.12175392364527911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0016678619677435494	0.0
14	0.0	0.0	0.0	0.0016678619677435494	0.0
15	0.0	0.0	0.0	0.0016678619677435494	0.0
16	0.0	0.0	0.0	0.0016678619677435494	0.0
17	0.0	0.0	0.0	0.0016678619677435494	0.0
18	0.0	0.0	0.0	0.0016678619677435494	0.0
19	0.0	0.0	0.0	0.003335723935487099	0.0
20	0.0	0.0	0.0	0.003335723935487099	0.0
21	0.0	0.0	0.0	0.003335723935487099	0.0
22	0.0	0.0	0.0	0.005003585903230649	0.0
23	0.0	0.0	0.0	0.010007171806461298	0.0
24	0.0	0.0	0.0	0.013342895741948396	0.0
25	0.0	0.0	0.0	0.016678619677435495	0.0
26	0.0	0.0	0.0	0.020014343612922595	0.0
27	0.0	0.0	0.0	0.03669296329035809	0.0
28	0.0	0.0	0.0	0.10007171806461297	0.0
29	0.0	0.0	0.0	0.21348633187117433	0.0
30	0.0	0.0	0.0	0.34191170338742766	0.0
31	0.0	0.0	0.0	0.6638090631619327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGGC	20	0.0018280569	37.0	12
GTATCAA	30	3.5592922E-4	30.833332	1
TCATGAC	25	0.0054554	29.6	3
CTCCAGA	40	0.0019100321	23.125	1
TATCAAC	45	0.0037842956	20.555557	2
TCAACGC	45	0.0037842956	20.555557	4
TCTTATA	55	5.06538E-4	20.181816	37
CAACGCA	50	0.006959279	18.5	5
CTCTTAT	105	9.051342E-6	15.857142	37
TGCAGAG	80	0.006207279	13.875	2
TCTCTTA	145	1.8804087E-4	11.482759	36
CCTGTCT	275	7.075869E-10	11.436364	37
GTCTCTT	225	8.8083107E-7	10.688889	37
TGTCTCT	335	1.8492756E-7	8.83582	37
CTGTCTC	430	1.0137974E-6	7.3139534	36
>>END_MODULE