FastQCFastQC Report
Fri 10 Feb 2017
ERR1632370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264982
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGT200.00183938137.017
ATTAGTA358.85132E-426.428572
CTCAATA358.85132E-426.4285732
ATAATAC358.85132E-426.428573
CAAGACA358.85132E-426.428574
TATTAAT451.3194613E-424.6666662
GTTAGCC400.001927288223.1250023
GTATAAT400.001927288223.1250021
GTACTGG502.6945054E-422.1999991
GGTATCA2100.022.0238111
GTATTAA555.1287503E-420.1818181
TAGCCCA555.1287503E-420.1818185
CCAAGAC656.876544E-519.9230773
ATACCCT609.2112675E-418.533
CCACAAG500.007020952618.49999819
ACAGTAC500.007020952618.4999983
GTTTAGG500.007020952618.4999981
TAACACA500.007020952618.4999984
GATCTCA500.007020952618.49999820
GATACCC650.001575600117.07692332