##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632370.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264982
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73558958721725	34.0	31.0	34.0	31.0	34.0
2	32.89973658588131	34.0	31.0	34.0	31.0	34.0
3	32.904340672196604	34.0	31.0	34.0	31.0	34.0
4	36.35551094036576	37.0	37.0	37.0	35.0	37.0
5	36.265089704206325	37.0	37.0	37.0	35.0	37.0
6	36.31037957295212	37.0	37.0	37.0	35.0	37.0
7	36.301201591051466	37.0	37.0	37.0	35.0	37.0
8	36.280913420534226	37.0	37.0	37.0	35.0	37.0
9	38.066310164464	39.0	38.0	39.0	35.0	39.0
10	37.98760293151987	39.0	38.0	39.0	35.0	39.0
11	38.100006792914236	39.0	38.0	39.0	37.0	39.0
12	38.062132522209055	39.0	38.0	39.0	35.0	39.0
13	38.09131941037504	39.0	38.0	39.0	37.0	39.0
14	39.470598002883214	40.0	39.0	41.0	37.0	41.0
15	39.50118498615	40.0	39.0	41.0	37.0	41.0
16	39.37701806160418	40.0	39.0	41.0	37.0	41.0
17	39.41814915730125	40.0	39.0	41.0	37.0	41.0
18	39.44244892105879	40.0	39.0	41.0	37.0	41.0
19	39.49419205832849	41.0	39.0	41.0	37.0	41.0
20	39.48612358575299	41.0	39.0	41.0	37.0	41.0
21	39.44553592319478	40.0	39.0	41.0	37.0	41.0
22	39.42485527318837	40.0	39.0	41.0	37.0	41.0
23	39.386203591187325	40.0	39.0	41.0	37.0	41.0
24	39.33205651704644	40.0	39.0	41.0	36.0	41.0
25	39.2702032590893	40.0	39.0	41.0	36.0	41.0
26	39.19849650164917	40.0	39.0	41.0	36.0	41.0
27	39.12272531719135	40.0	39.0	41.0	36.0	41.0
28	39.04584084956714	40.0	39.0	41.0	36.0	41.0
29	38.909688205236584	40.0	39.0	41.0	35.0	41.0
30	38.871425228883474	40.0	38.0	41.0	35.0	41.0
31	38.796529575593816	40.0	38.0	41.0	35.0	41.0
32	38.74765078382683	40.0	38.0	41.0	35.0	41.0
33	38.6109358371512	40.0	38.0	41.0	35.0	41.0
34	38.61266048259882	40.0	38.0	41.0	35.0	41.0
35	38.58981742156071	40.0	38.0	41.0	35.0	41.0
36	38.567683842676104	40.0	38.0	41.0	35.0	41.0
37	38.53967816681888	40.0	38.0	41.0	35.0	41.0
38	38.47188488274675	40.0	38.0	41.0	34.0	41.0
39	38.29858631907073	40.0	38.0	41.0	34.0	41.0
40	38.30094496984701	40.0	38.0	41.0	34.0	41.0
41	38.30269603218332	40.0	38.0	41.0	34.0	41.0
42	38.17631008898718	40.0	38.0	41.0	34.0	41.0
43	37.31890468031791	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	3.0
19	12.0
20	18.0
21	31.0
22	60.0
23	111.0
24	175.0
25	247.0
26	385.0
27	593.0
28	870.0
29	1175.0
30	1607.0
31	2196.0
32	2990.0
33	4415.0
34	6446.0
35	9787.0
36	15863.0
37	28555.0
38	67265.0
39	122172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98782558815316	18.320867077763772	12.188375059437998	26.50293227464507
2	20.598380267338914	19.95456295144576	32.229736359450825	27.217320421764494
3	21.169739831384774	20.998407438995855	28.917058517182298	28.914794212437073
4	15.611626450098498	14.979130657931483	33.46944320746315	35.93979968450687
5	16.741891902091464	34.0083477368274	32.74826214610804	16.501498214973093
6	36.63154478417402	33.261881939150584	14.523628020016455	15.582945256658942
7	30.37036477949446	28.979704281800274	20.190050644949466	20.459880293755802
8	28.847997222452847	30.756428738555826	19.38584507626933	21.009728962721997
9	27.539229079711074	13.237125540602756	19.246590334437812	39.977055045248356
10	18.53408910793941	25.060570151934847	31.75045852171091	24.654882218414837
11	37.79464265497279	20.3138326376886	19.56623468763916	22.32529001969945
12	23.734819723603866	24.57827324120129	26.586711550218507	25.10019548497634
13	32.86034523099682	18.216331675359083	22.53775728162668	26.38556581201742
14	24.34278554769758	19.727377708674553	23.17968767689881	32.750149066729065
15	27.64150017737054	26.00667215131594	20.297982504472	26.053845166841523
16	27.064102467337403	24.415243299544876	22.06376282162562	26.4568914114921
17	25.62475941762082	24.388449026726345	23.60160312775962	26.385188427893212
18	25.73382342951597	23.22044516231291	24.585820923685382	26.45991048448574
19	26.653885924326936	23.405740767297402	24.521288238446385	25.419085069929277
20	27.516963416383	22.863439780815302	23.672551343110097	25.947045459691605
21	27.73962004966375	23.381588183348303	22.99024084654807	25.88855092043988
22	27.649425243978836	23.197047346612223	23.29554460302964	25.857982806379304
23	26.90522375104724	22.790982028968003	23.23138930191485	27.07240491806991
24	26.338015412367632	23.477066366772082	23.678212104973166	26.50670611588712
25	26.467835551094037	23.2276154606728	23.400080005434333	26.90446898279883
26	26.340279717112857	23.631039089447587	23.2762980126952	26.752383180744353
27	26.394245646874126	23.22195469880973	23.74878293619944	26.6350167181167
28	26.007426919564345	23.392909707074445	23.760859228173988	26.838804145187222
29	25.4534270252319	23.65254998452725	24.59638767916311	26.297635311077734
30	25.87760678083794	24.0691820576492	23.852186186231517	26.20102497528134
31	26.32895819338672	24.111826463684324	23.34800099629409	26.211214346634865
32	25.121706380056004	23.851808802107314	23.77067121540331	27.25581360243337
33	24.84130997577194	23.71783743801466	24.580160161822313	26.860692424391093
34	25.978745726124792	22.699655070910477	24.66507158976836	26.656527613196367
35	25.51682755809829	23.143081416850954	24.74658656059657	26.59350446445419
36	25.210014265119895	23.80690009132696	24.40278962344612	26.580296020107024
37	25.402857552588475	22.59474228438158	25.40059324784325	26.601806915186692
38	24.851121963001262	22.615121027088634	25.76741061657018	26.766346393339923
39	24.598274599784137	22.113577526020638	26.714644768323886	26.573503105871342
40	23.858601716342996	21.656187967484584	27.742261738533188	26.742948577639236
41	23.54650504562574	21.657697503981403	28.181159474983208	26.61463797540965
42	23.423855205259226	21.57240869191115	28.583073567261174	26.42066253556845
43	22.3049112769924	21.111245292133052	29.120468560128614	27.463374870745938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	9.0
2	15.0
3	17.0
4	19.0
5	19.0
6	39.5
7	60.0
8	48.0
9	36.0
10	66.5
11	97.0
12	97.0
13	141.5
14	186.0
15	318.0
16	450.0
17	465.5
18	481.0
19	481.0
20	631.5
21	782.0
22	911.5
23	1041.0
24	1319.0
25	1597.0
26	1597.0
27	2023.0
28	2449.0
29	2975.5
30	3502.0
31	4354.5
32	5207.0
33	5207.0
34	6230.5
35	7254.0
36	8489.5
37	9725.0
38	10459.0
39	11193.0
40	11193.0
41	12059.5
42	12926.0
43	13890.5
44	14855.0
45	15597.0
46	16339.0
47	16339.0
48	17072.0
49	17805.0
50	18440.5
51	19076.0
52	19798.5
53	20521.0
54	20521.0
55	20450.0
56	20379.0
57	20534.5
58	20690.0
59	20012.0
60	19334.0
61	19334.0
62	17670.5
63	16007.0
64	14589.0
65	13171.0
66	11559.0
67	9947.0
68	9947.0
69	8679.5
70	7412.0
71	6175.5
72	4939.0
73	3872.0
74	2805.0
75	2805.0
76	2213.0
77	1621.0
78	1348.5
79	1076.0
80	969.0
81	862.0
82	862.0
83	656.5
84	451.0
85	383.0
86	315.0
87	244.5
88	174.0
89	174.0
90	148.5
91	123.0
92	77.5
93	32.0
94	22.5
95	13.0
96	13.0
97	11.5
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	264982.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.47390937720527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.12637782521884	70.4526426905596
2	4.279661258478481	6.545648544920393
3	1.205464494922242	2.765597476263662
4	0.619643909444249	1.8954636870790675
5	0.3726833354698777	1.425027581155901
6	0.23615677054319462	1.0835898881600237
7	0.18089176598012816	0.9683450363052861
8	0.13790744681774308	0.8437057272305473
9	0.09963698985638737	0.6857667119605401
>10	0.6849726430260963	9.913024342042862
>50	0.04617658861911723	2.2945883444449087
>100	0.010426971623671621	1.1265999698772196
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.7738412420466297E-4	0.0	0.0	0.0
4	0.0	3.7738412420466297E-4	0.0	0.0	0.0
5	0.0	3.7738412420466297E-4	0.0	0.0	0.0
6	0.0	3.7738412420466297E-4	0.0	0.0	0.0
7	0.0	3.7738412420466297E-4	0.0	0.0	0.0
8	0.0	3.7738412420466297E-4	0.0	0.0	0.0
9	0.0	3.7738412420466297E-4	0.0	0.0	0.0
10	0.0	3.7738412420466297E-4	0.0	0.0	0.0
11	0.0	3.7738412420466297E-4	0.0	0.0	0.0
12	0.0	3.7738412420466297E-4	0.0	0.0	0.0
13	0.0	3.7738412420466297E-4	0.0	0.0	0.0
14	0.0	3.7738412420466297E-4	0.0	0.0	0.0
15	0.0	3.7738412420466297E-4	0.0	3.7738412420466297E-4	0.0
16	0.0	3.7738412420466297E-4	0.0	0.0015095364968186519	0.0
17	0.0	3.7738412420466297E-4	0.0	0.0033964571178419665	0.0
18	0.0	3.7738412420466297E-4	0.0	0.004151225366251293	0.0
19	0.0	3.7738412420466297E-4	0.0	0.004528609490455956	0.0
20	0.0	3.7738412420466297E-4	0.0	0.004528609490455956	0.0
21	0.0	3.7738412420466297E-4	0.0	0.00641553011147927	0.0
22	0.0	3.7738412420466297E-4	0.0	0.009057218980911911	0.0
23	0.0	3.7738412420466297E-4	0.0	0.009811987229321237	0.0
24	0.0	3.7738412420466297E-4	0.0	0.013208444347163204	0.0
25	0.0	3.7738412420466297E-4	0.0	0.015850133216595844	0.0
26	0.0	3.7738412420466297E-4	0.0	0.022265663328075114	0.0
27	0.0	3.7738412420466297E-4	0.0	0.038870564793080285	0.0
28	0.0	3.7738412420466297E-4	0.0	0.11019616426776159	0.0
29	0.0	3.7738412420466297E-4	0.0	0.203787427070518	0.0
30	0.0	3.7738412420466297E-4	0.0	0.3309658769274894	0.0
31	0.0	3.7738412420466297E-4	0.0	0.6223064208134892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGT	20	0.001839381	37.0	17
ATTAGTA	35	8.85132E-4	26.42857	2
CTCAATA	35	8.85132E-4	26.42857	32
ATAATAC	35	8.85132E-4	26.42857	3
CAAGACA	35	8.85132E-4	26.42857	4
TATTAAT	45	1.3194613E-4	24.666666	2
GTTAGCC	40	0.0019272882	23.125002	3
GTATAAT	40	0.0019272882	23.125002	1
GTACTGG	50	2.6945054E-4	22.199999	1
GGTATCA	210	0.0	22.023811	1
GTATTAA	55	5.1287503E-4	20.181818	1
TAGCCCA	55	5.1287503E-4	20.181818	5
CCAAGAC	65	6.876544E-5	19.923077	3
ATACCCT	60	9.2112675E-4	18.5	33
CCACAAG	50	0.0070209526	18.499998	19
ACAGTAC	50	0.0070209526	18.499998	3
GTTTAGG	50	0.0070209526	18.499998	1
TAACACA	50	0.0070209526	18.499998	4
GATCTCA	50	0.0070209526	18.499998	20
GATACCC	65	0.0015756001	17.076923	32
>>END_MODULE