FastQCFastQC Report
Fri 10 Feb 2017
ERR1632369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995737
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.15847558140352322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12040.12091546261713684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11470.11519105948659134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5500.026.2363621
ATTACAC806.9581984E-720.81253
GTATTAA2500.019.981
ACGGACC1052.2584572E-819.380958
ATAGGCG701.21898935E-418.53
CTCTATC2050.018.0487829
CTATACT1351.1514203E-917.8148164
AACTTAT1900.017.52631631
ATCTGCG1800.017.47222136
ATAAGAC852.7225236E-517.4117643
CTTATAC5400.017.1296337
ACGGGAC650.001579733817.0769213
TAGAGTC1757.2759576E-1216.9142865
TATTAAT2900.016.5862062
TTAACGA904.444997E-516.4444454
ATCTCTA2500.016.2799997
TATACTG1251.6569356E-716.2799995
CGAACGA700.00259216715.85714316
CTATCCG700.00259216715.8571439
GTATACC700.00259216715.8571431
Produced by FastQC (version 0.11.5)