FastQCFastQC Report
Fri 10 Feb 2017
ERR1632368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT2112.4395883917215864No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT1681.9424210891432536No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC1371.5839981500751534No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC1341.549312059197595No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG660.7630939993062782No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC580.6705977569661232Illumina PCR Primer Index 7 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT440.5087293328708521No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG390.4509191814082553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT380.4393571511157359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230.26592669672794544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.19655451497282922No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA120.1387443635102324No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC110.12718233321771302No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG100.11562030292519365No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGT90.10405827263267431No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT90.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC251.1347575E-437.035
ATGCCGT251.1347575E-437.034
GCCGTCT251.1347575E-437.036
GTATGCC303.3135884E-430.83333232
TATGCCG303.3135884E-430.83333233
CCGTCTT303.3135884E-430.83333237
GGTATCA652.2064341E-928.4615381
TCTCGTA358.1712875E-426.42857228
CGTATGC358.1712875E-426.42857231
TCGTATG358.1712875E-426.42857230
CTCGTAT358.1712875E-426.42857229
GTATCAA802.0205334E-823.1252
CAACGCA907.046947E-820.5555556
AACGCAG951.2480268E-719.4736847
TCAACGC951.2480268E-719.4736845
TATCAAC951.2480268E-719.4736843
ATCAACG951.2480268E-719.4736844
ATCTCGT500.00649615218.527
AGTACAT903.679716E-516.44444514
GAGTACA955.8499354E-515.57894813
Produced by FastQC (version 0.11.5)