##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632368.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8649
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74297606659729	34.0	31.0	34.0	31.0	34.0
2	32.89663544918488	34.0	31.0	34.0	31.0	34.0
3	32.86449300497167	34.0	31.0	34.0	31.0	34.0
4	36.37322233784253	37.0	37.0	37.0	35.0	37.0
5	36.27286391490345	37.0	35.0	37.0	35.0	37.0
6	36.314718464562375	37.0	37.0	37.0	35.0	37.0
7	36.33055844606313	37.0	36.0	37.0	35.0	37.0
8	36.304428257602034	37.0	37.0	37.0	35.0	37.0
9	38.07596253902185	39.0	39.0	39.0	37.0	39.0
10	38.04416695571742	39.0	38.0	39.0	37.0	39.0
11	38.12695109261186	39.0	38.0	39.0	37.0	39.0
12	38.090299456584575	39.0	38.0	39.0	37.0	39.0
13	38.21574748525841	39.0	39.0	39.0	37.0	39.0
14	39.506995028326976	40.0	39.0	41.0	37.0	41.0
15	39.477743091686904	40.0	39.0	41.0	37.0	41.0
16	39.31541218637993	40.0	39.0	41.0	37.0	41.0
17	39.3873280147994	40.0	39.0	41.0	37.0	41.0
18	39.526303618915485	40.0	39.0	41.0	37.0	41.0
19	39.577292172505494	41.0	39.0	41.0	37.0	41.0
20	39.59810382703203	41.0	39.0	41.0	37.0	41.0
21	39.54434038617181	41.0	39.0	41.0	37.0	41.0
22	39.437738466874784	40.0	39.0	41.0	37.0	41.0
23	39.48040235865418	40.0	39.0	41.0	37.0	41.0
24	39.3882529772228	40.0	39.0	41.0	37.0	41.0
25	39.274366978841485	40.0	39.0	41.0	36.0	41.0
26	39.225575211007055	40.0	39.0	41.0	36.0	41.0
27	39.01676494392415	40.0	39.0	41.0	35.0	41.0
28	39.08821829113192	40.0	39.0	41.0	35.0	41.0
29	38.83894091802521	40.0	38.0	41.0	35.0	41.0
30	38.79361775927853	40.0	38.0	41.0	35.0	41.0
31	38.72170193085906	40.0	38.0	41.0	35.0	41.0
32	38.56330211585154	40.0	38.0	41.0	35.0	41.0
33	38.50630130650942	40.0	38.0	41.0	35.0	41.0
34	38.4910394265233	40.0	38.0	41.0	35.0	41.0
35	38.395537056307084	40.0	38.0	41.0	34.0	41.0
36	38.397502601456814	40.0	38.0	41.0	34.0	41.0
37	38.329286622730955	40.0	38.0	41.0	34.0	41.0
38	38.282807260955025	40.0	38.0	41.0	34.0	41.0
39	38.00890276332524	40.0	37.0	41.0	34.0	41.0
40	37.941264886114	40.0	37.0	41.0	33.0	41.0
41	37.95987975488496	40.0	37.0	41.0	34.0	41.0
42	37.763672100820905	40.0	37.0	41.0	33.0	41.0
43	36.85420279801133	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	2.0
24	5.0
25	8.0
26	10.0
27	18.0
28	21.0
29	35.0
30	53.0
31	65.0
32	106.0
33	164.0
34	223.0
35	340.0
36	586.0
37	1058.0
38	2221.0
39	3732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.021852237252865	18.198635680425483	16.383396924499944	26.396115157821715
2	19.065787952364435	18.927043588854204	35.160134119551394	26.84703433922997
3	18.64955486183374	22.106601919297027	36.420395421436005	22.82344779743323
4	14.175049138628742	19.007977800901838	29.07850618568621	37.738466874783214
5	13.966932593363396	33.05584460631287	34.61671869580299	18.360504104520754
6	31.078737426292058	32.82460400046248	16.152156318649556	19.944502254595907
7	24.24557752341311	31.42559833506764	18.6264308012487	25.70239334027055
8	30.30408139669326	30.188461093768066	19.52826916406521	19.979188345473464
9	24.673372644236327	12.209503988900451	16.718695802983007	46.39842756388022
10	20.487917678344317	24.731182795698924	25.436466643542605	29.34443288241415
11	33.42582957567349	21.14695340501792	18.29113192276564	27.136085096542956
12	20.141056769568735	28.98600994334605	24.176205341657994	26.696727945427217
13	34.397040120245116	16.880564227078278	22.673141403630478	26.049254249046133
14	25.112729795352063	21.424442132038386	21.70193085905885	31.7608972135507
15	28.44259451959764	23.887154584345012	19.16984622499711	28.500404671060238
16	27.99167533818939	22.65001734304544	22.014105676956873	27.3442016418083
17	20.67291016302463	32.327436697884146	21.320383859405712	25.679269279685514
18	25.170539946814664	19.63232743669788	24.950861371256792	30.24627124523066
19	25.078043704474506	25.170539946814664	26.662041854549656	23.089374494161174
20	26.268932824604	20.97352295063013	23.864030523759972	28.893513701005897
21	32.99803445485027	20.106370678691178	22.152850040467108	24.742744825991444
22	26.65047982425714	26.44236327899179	21.620996647011214	25.286160249739854
23	27.58700427795121	20.67291016302463	22.76563764597063	28.97444791305353
24	26.927968551277605	23.875592554052492	24.846803098624118	24.349635796045785
25	22.61533125216788	23.89871661463753	24.835241068331598	28.65071106486299
26	28.720083246618106	21.805989131691526	22.31471846456238	27.15920915712799
27	23.401549312059196	26.638917793964623	24.511504220141056	25.448028673835125
28	23.783096311712338	20.696034223609665	25.494276795005206	30.026592669672798
29	24.742744825991444	23.239680887963925	26.719852006012257	25.297722280032374
30	22.337842525147416	21.655682737888775	29.51786333680194	26.488611400161872
31	26.153312521678806	21.320383859405712	23.736848190542258	28.789455428373223
32	24.534628280726096	24.661810613943807	28.523528731645275	22.28003237368482
33	22.118163949589547	22.545959070412763	28.14198173199214	27.19389524800555
34	25.586773037345356	24.153081281072954	24.962423401549312	25.297722280032374
35	23.112498554746214	21.066019192970288	32.604925424904614	23.216556827378888
36	23.52873164527691	22.800323736848192	24.765868886576484	28.905075731298417
37	21.944733495201756	23.124060585038734	27.02046479361776	27.91074112614175
38	23.031564342698577	19.73638570933056	29.379118973291714	27.85293097467915
39	23.736848190542258	20.626662041854548	28.500404671060238	27.136085096542956
40	20.82321655682738	18.372066134813274	34.397040120245116	26.40767718811423
41	21.875361313446643	19.782633830500636	29.853162215285007	28.48884264076772
42	21.100705283847844	18.846109376806567	34.096427332639614	25.956758006705975
43	18.742051104173893	19.6438894669904	32.84772806104752	28.766331367788183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	6.0
6	7.0
7	8.0
8	7.0
9	6.0
10	10.0
11	14.0
12	14.0
13	29.5
14	45.0
15	57.5
16	70.0
17	63.5
18	57.0
19	57.0
20	60.5
21	64.0
22	45.5
23	27.0
24	29.0
25	31.0
26	31.0
27	40.5
28	50.0
29	61.0
30	72.0
31	87.0
32	102.0
33	102.0
34	110.5
35	119.0
36	133.0
37	147.0
38	195.5
39	244.0
40	244.0
41	284.0
42	324.0
43	401.0
44	478.0
45	570.0
46	662.0
47	662.0
48	774.5
49	887.0
50	837.5
51	788.0
52	892.0
53	996.0
54	996.0
55	798.5
56	601.0
57	773.0
58	945.0
59	809.5
60	674.0
61	674.0
62	521.5
63	369.0
64	311.0
65	253.0
66	221.5
67	190.0
68	190.0
69	165.0
70	140.0
71	122.0
72	104.0
73	91.0
74	78.0
75	78.0
76	62.0
77	46.0
78	37.0
79	28.0
80	20.5
81	13.0
82	13.0
83	11.5
84	10.0
85	5.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8649.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.28673835125448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.64112903225806	74.37854087177709
2	5.256336405529954	8.440282113539139
3	1.065668202764977	2.5667707249392993
4	0.3744239631336405	1.2024511504220141
5	0.20161290322580644	0.8093421204763557
6	0.14400921658986177	0.693721817551162
7	0.0576036866359447	0.32373684819054227
8	0.02880184331797235	0.18499248468030985
9	0.02880184331797235	0.20811654526534862
>10	0.1152073732718894	2.243033876748757
>50	0.02880184331797235	1.4336917562724014
>100	0.0576036866359447	7.515319690137588
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	211	2.4395883917215864	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	168	1.9424210891432536	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	137	1.5839981500751534	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	134	1.549312059197595	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	66	0.7630939993062782	No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC	58	0.6705977569661232	Illumina PCR Primer Index 7 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.5087293328708521	No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG	39	0.4509191814082553	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.4393571511157359	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.26592669672794544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.19655451497282922	No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA	12	0.1387443635102324	No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC	11	0.12718233321771302	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	10	0.11562030292519365	No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGT	9	0.10405827263267431	No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT	9	0.10405827263267431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.011562030292519366	0.0
22	0.0	0.0	0.0	0.011562030292519366	0.0
23	0.0	0.0	0.0	0.011562030292519366	0.0
24	0.0	0.0	0.0	0.02312406058503873	0.0
25	0.0	0.0	0.0	0.02312406058503873	0.0
26	0.0	0.0	0.0	0.0346860908775581	0.0
27	0.0	0.0	0.0	0.05781015146259683	0.0
28	0.0	0.0	0.0	0.15030639380275176	0.0
29	0.0	0.0	0.0	0.40467106023817784	0.0
30	0.0	0.0	0.0	0.72840790842872	0.0
31	0.0	0.0	0.0	1.4105676956873627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	25	1.1347575E-4	37.0	35
ATGCCGT	25	1.1347575E-4	37.0	34
GCCGTCT	25	1.1347575E-4	37.0	36
GTATGCC	30	3.3135884E-4	30.833332	32
TATGCCG	30	3.3135884E-4	30.833332	33
CCGTCTT	30	3.3135884E-4	30.833332	37
GGTATCA	65	2.2064341E-9	28.461538	1
TCTCGTA	35	8.1712875E-4	26.428572	28
CGTATGC	35	8.1712875E-4	26.428572	31
TCGTATG	35	8.1712875E-4	26.428572	30
CTCGTAT	35	8.1712875E-4	26.428572	29
GTATCAA	80	2.0205334E-8	23.125	2
CAACGCA	90	7.046947E-8	20.555555	6
AACGCAG	95	1.2480268E-7	19.473684	7
TCAACGC	95	1.2480268E-7	19.473684	5
TATCAAC	95	1.2480268E-7	19.473684	3
ATCAACG	95	1.2480268E-7	19.473684	4
ATCTCGT	50	0.006496152	18.5	27
AGTACAT	90	3.679716E-5	16.444445	14
GAGTACA	95	5.8499354E-5	15.578948	13
>>END_MODULE