FastQCFastQC Report
Fri 10 Feb 2017
ERR1632366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27604
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT10483.7965512244602233RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT6212.2496739602956093No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC5051.8294450079698594RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC4491.6265758585712216No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG3641.3186494710911463No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC2811.017968410375308RNA PCR Primer, Index 29 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1890.6846833792204028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.5760034777568469No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG1450.5252861904071874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1120.40573829879727574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.1956238226344008No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT430.15577452543109693No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT410.14852919866685987No Hit
ATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT410.14852919866685987Illumina PCR Primer Index 10 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA290.10505723808143747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC251.2026881E-437.037
GGTATCA1550.029.838711
TAAGGCG250.005405048429.67
CCGTCTT1350.028.77777937
GCCGTCT1400.027.7536
CTTATAC856.184564E-1126.1176471
TTATACA901.2551027E-1024.6666682
TCTTATA652.5289846E-622.7692337
GTATCAA2050.022.5609742
TATACAC1004.7111826E-1022.23
TGCCGTC1900.020.44736935
ATGCCGT1900.020.44736934
TATGCCG2000.019.42500133
CTCTTAT801.5256441E-518.536
GTATGCC2350.016.53191632
CGTATGC2500.015.5431
ATACACA1701.2587407E-915.2352941
TACACAT1609.502401E-915.031252
CATGGGA1252.714156E-614.79999918
TATCAAC3250.014.2307693
Produced by FastQC (version 0.11.5)