##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632366.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27604
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52771337487321	33.0	31.0	34.0	31.0	34.0
2	32.671243298072746	34.0	31.0	34.0	31.0	34.0
3	32.59915229676859	34.0	31.0	34.0	31.0	34.0
4	36.150014490653525	37.0	35.0	37.0	35.0	37.0
5	36.106941023040136	37.0	35.0	37.0	35.0	37.0
6	36.185081872192434	37.0	35.0	37.0	35.0	37.0
7	36.14664541370816	37.0	35.0	37.0	35.0	37.0
8	36.15631792493841	37.0	35.0	37.0	35.0	37.0
9	37.89512389508767	39.0	38.0	39.0	35.0	39.0
10	37.80919431966382	39.0	37.0	39.0	35.0	39.0
11	37.94268946529488	39.0	38.0	39.0	35.0	39.0
12	37.86433125633966	39.0	38.0	39.0	35.0	39.0
13	37.949862338791476	39.0	38.0	39.0	35.0	39.0
14	39.10900594116794	40.0	38.0	41.0	36.0	41.0
15	39.123931314302276	40.0	38.0	41.0	36.0	41.0
16	38.96924358788581	40.0	38.0	41.0	36.0	41.0
17	39.046551224460224	40.0	38.0	41.0	36.0	41.0
18	39.227793073467616	40.0	39.0	41.0	36.0	41.0
19	39.23308216200551	40.0	39.0	41.0	36.0	41.0
20	39.29691349079844	40.0	39.0	41.0	36.0	41.0
21	39.3079626141139	40.0	39.0	41.0	36.0	41.0
22	39.181060715838285	40.0	39.0	41.0	36.0	41.0
23	39.218337922040284	40.0	39.0	41.0	36.0	41.0
24	39.10632517026518	40.0	39.0	41.0	36.0	41.0
25	38.95087668453847	40.0	38.0	41.0	35.0	41.0
26	38.83973337197508	40.0	38.0	41.0	35.0	41.0
27	38.73569047964063	40.0	38.0	41.0	35.0	41.0
28	38.743515432546005	40.0	38.0	41.0	35.0	41.0
29	38.584879003043035	40.0	38.0	41.0	35.0	41.0
30	38.54839878278511	40.0	38.0	41.0	35.0	41.0
31	38.42551804086364	40.0	38.0	41.0	34.0	41.0
32	38.3392624257354	40.0	38.0	41.0	34.0	41.0
33	38.17265613679177	40.0	38.0	41.0	34.0	41.0
34	38.078177075786115	40.0	37.0	41.0	34.0	41.0
35	38.02894508042313	40.0	37.0	41.0	34.0	41.0
36	37.98040139110274	40.0	37.0	41.0	34.0	41.0
37	37.9437400376757	40.0	37.0	41.0	34.0	41.0
38	37.88027097522098	40.0	37.0	41.0	33.0	41.0
39	37.658165483263296	40.0	37.0	41.0	33.0	41.0
40	37.5689030575279	40.0	37.0	41.0	33.0	41.0
41	37.58277785828141	40.0	37.0	41.0	33.0	41.0
42	37.38135777423562	40.0	36.0	41.0	33.0	41.0
43	36.48746558469787	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	7.0
23	14.0
24	26.0
25	38.0
26	55.0
27	63.0
28	135.0
29	175.0
30	261.0
31	350.0
32	439.0
33	545.0
34	765.0
35	1305.0
36	2079.0
37	3669.0
38	7768.0
39	9909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.54528329227648	19.75800608607448	18.60237646717867	24.094334154470367
2	18.05173163309665	20.35212288074192	34.194319663816835	27.401825822344588
3	17.60976670047819	23.188668308940734	37.82060570931749	21.380959281263586
4	12.284451528763947	18.84871757716273	28.039414577597448	40.827416316475876
5	12.577887262715548	35.00579626141139	33.5023909578322	18.913925518040863
6	30.140559339226197	32.6619330531807	15.265903492247501	21.9316041153456
7	23.188668308940734	34.5203593682075	17.475728155339805	24.815244167511956
8	32.83582089552239	28.753079263874803	19.153021301260686	19.258078539342126
9	23.598029271120126	12.335168816113606	17.58803072018548	46.47877119258079
10	20.888277061295465	24.89494276191856	25.978119113172006	28.238661063613968
11	32.154760179684104	20.540501376612085	17.76554122590929	29.539197217794523
12	19.41747572815534	32.16925083321257	21.35922330097087	27.054050137661207
13	35.04926822199681	16.443269091436026	22.768439356614984	25.739023329952182
14	25.31517171424431	23.89146500507173	22.112737284451526	28.68062599623243
15	30.104332705405014	25.032603970439066	19.058832053325606	25.804231270830314
16	29.234893493696568	22.417041008549486	22.04028401680916	26.30778148094479
17	19.79061005651355	34.51673670482539	19.750760759310246	25.94189247935082
18	24.15591943196638	19.649326184610928	24.539921750470945	31.654832632951745
19	25.416606288943633	25.873061875090563	27.115635415157225	21.594696420808578
20	29.013911027387334	19.239965222431533	24.496449789885524	27.249673960295613
21	31.81785248514708	18.645848427764093	22.583683524126936	26.95261556296189
22	29.62251847558325	27.401825822344588	20.982466309230546	21.993189392841618
23	30.36878713229967	19.518910302854657	21.74322561947544	28.369076945370235
24	25.30430372409796	23.819011737429356	24.673960295609334	26.20272424286335
25	25.101434574699322	24.789885523837125	25.847703231415736	24.260976670047818
26	24.445732502535865	25.452832922764816	23.724822489494276	26.376612085205043
27	23.40965077524996	28.18794377626431	25.52166352702507	22.880741921460658
28	22.50036226633821	22.681495435444138	27.267787277206203	27.55035502101145
29	21.529488479930446	24.217504709462396	26.8765396319374	27.376467178669756
30	24.981886683089407	22.21417185915085	30.227503260397043	22.576438197362698
31	22.112737284451526	23.66323721199826	22.82640197072888	31.397623532821328
32	23.127083031444716	25.76438197362701	28.745833937110564	22.36270105781771
33	22.420663671931603	23.645123895087668	29.923199536299087	24.011012896681642
34	22.808288653818288	25.731778003187944	25.854948558179974	25.604984784813794
35	21.19982611215766	21.44978988552384	35.023909578321984	22.326474423996522
36	23.91320098536444	25.46732357629329	24.692073612519923	25.927401825822344
37	20.420953485002176	23.500217359802928	28.398058252427184	27.680770902767716
38	21.630923054629765	20.4064628314737	29.213157513403853	28.749456600492685
39	24.000144906535283	20.90639037820606	28.67338066946819	26.420084045790464
40	19.290682509781192	18.65671641791045	36.30270975220983	25.749891320098538
41	21.783074916678743	20.1130270975221	29.061005651354876	29.042892334444282
42	19.950007245326766	19.102304013911027	36.11433125633966	24.833357484422546
43	18.015504999275468	19.051586726561368	34.596435299232	28.33647297493117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	7.5
4	13.0
5	13.0
6	17.0
7	21.0
8	22.0
9	23.0
10	41.0
11	59.0
12	59.0
13	109.0
14	159.0
15	234.5
16	310.0
17	277.0
18	244.0
19	244.0
20	283.0
21	322.0
22	219.5
23	117.0
24	124.5
25	132.0
26	132.0
27	139.5
28	147.0
29	166.0
30	185.0
31	206.5
32	228.0
33	228.0
34	264.0
35	300.0
36	367.0
37	434.0
38	540.5
39	647.0
40	647.0
41	788.5
42	930.0
43	1218.5
44	1507.0
45	1839.0
46	2171.0
47	2171.0
48	2512.0
49	2853.0
50	3314.0
51	3775.0
52	3171.0
53	2567.0
54	2567.0
55	3136.0
56	3705.0
57	3189.0
58	2673.0
59	1972.5
60	1272.0
61	1272.0
62	1111.5
63	951.0
64	807.5
65	664.0
66	543.5
67	423.0
68	423.0
69	355.0
70	287.0
71	234.5
72	182.0
73	157.5
74	133.0
75	133.0
76	111.5
77	90.0
78	63.5
79	37.0
80	33.0
81	29.0
82	29.0
83	18.0
84	7.0
85	5.0
86	3.0
87	1.5
88	0.0
89	0.0
90	1.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	27604.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.66657006230982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.07641116483939	69.67106216490365
2	5.611145688705894	8.491522967685842
3	1.2304304112605926	2.7930734676133895
4	0.43089002728970177	1.3041588175626722
5	0.2681093503135922	1.0143457469931894
6	0.11969167424713938	0.54339950731778
7	0.05266433666874132	0.27894508042312705
8	0.03351366878919902	0.20286914939863787
9	0.02872600181931345	0.1956238226344008
>10	0.0957533393977115	1.2788001738878423
>50	0.004787666969885574	0.1956238226344008
>100	0.03351366878919902	6.154905086219388
>500	0.009575333939771148	4.079118968265469
>1k	0.004787666969885574	3.7965512244602233
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	1048	3.7965512244602233	RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	621	2.2496739602956093	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	505	1.8294450079698594	RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	449	1.6265758585712216	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	364	1.3186494710911463	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	281	1.017968410375308	RNA PCR Primer, Index 29 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.6846833792204028	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	159	0.5760034777568469	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	145	0.5252861904071874	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	112	0.40573829879727574	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.1956238226344008	No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT	43	0.15577452543109693	No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT	41	0.14852919866685987	No Hit
ATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT	41	0.14852919866685987	Illumina PCR Primer Index 10 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA	29	0.10505723808143747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0036226633821185334	0.0
20	0.0	0.0	0.0	0.0036226633821185334	0.0
21	0.0	0.0	0.0	0.0036226633821185334	0.0
22	0.0	0.0	0.0	0.0036226633821185334	0.0
23	0.0	0.0	0.0	0.0108679901463556	0.0
24	0.0	0.0	0.0	0.025358643674829734	0.0
25	0.0	0.0	0.0	0.036226633821185336	0.0
26	0.0	0.0	0.0	0.0434719605854224	0.0
27	0.0	0.0	0.0	0.07969859440660773	0.0
28	0.0	0.0	0.0	0.15939718881321546	0.0
29	0.0	0.0	0.0	0.3332850311549051	0.0
30	0.0	0.0	0.0	0.6557020721634546	0.0
31	0.0	0.0	0.0	1.3222721344732646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	25	1.2026881E-4	37.0	37
GGTATCA	155	0.0	29.83871	1
TAAGGCG	25	0.0054050484	29.6	7
CCGTCTT	135	0.0	28.777779	37
GCCGTCT	140	0.0	27.75	36
CTTATAC	85	6.184564E-11	26.117647	1
TTATACA	90	1.2551027E-10	24.666668	2
TCTTATA	65	2.5289846E-6	22.76923	37
GTATCAA	205	0.0	22.560974	2
TATACAC	100	4.7111826E-10	22.2	3
TGCCGTC	190	0.0	20.447369	35
ATGCCGT	190	0.0	20.447369	34
TATGCCG	200	0.0	19.425001	33
CTCTTAT	80	1.5256441E-5	18.5	36
GTATGCC	235	0.0	16.531916	32
CGTATGC	250	0.0	15.54	31
ATACACA	170	1.2587407E-9	15.235294	1
TACACAT	160	9.502401E-9	15.03125	2
CATGGGA	125	2.714156E-6	14.799999	18
TATCAAC	325	0.0	14.230769	3
>>END_MODULE