FastQCFastQC Report
Fri 10 Feb 2017
ERR1632365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64864
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13072.014985199802664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11991.8484829797730635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8161.2580167735569807No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT5540.85409472126295TruSeq Adapter, Index 8 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.713801184015787No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT3670.5657992106561421No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.3715466206216083No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC2230.3437962506166749No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC2070.3191292550567341No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG1830.2821287617168229No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.24358658115441542No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC1340.20658608781450422RNA PCR Primer, Index 8 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.17883571780957078No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1000.15416872224963No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.1295017266896892No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1110014800197336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG690.1063764183522447No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.1017513566847558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGA358.7790936E-426.4285721
AAGCATG400.001911711523.12500224
GTACCTG450.00378759320.55555735
GAAGCAT450.00378759320.55555723
GGTATCA6900.020.3768121
GTTCCGA555.0715404E-420.1818181
TAGGACC500.006965284718.536
TATACAC609.109356E-418.53
CTTAGGA500.006965284718.534
CACGAAT500.006965284718.512
CTTATTG609.109356E-418.528
GGCCACA500.006965284718.530
CCTTATT609.109356E-418.527
CCTTAGG500.006965284718.533
TGGCGTA500.006965284718.518
ACGATGG500.006965284718.525
ACGAATG500.006965284718.513
GCCGTCT752.029634E-417.26666636
TTCCTCG650.001558310217.07692120
AATGGCG650.001558310217.07692116
Produced by FastQC (version 0.11.5)