##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64864
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86073939319191	31.0	31.0	34.0	30.0	34.0
2	31.983118524913664	33.0	31.0	34.0	30.0	34.0
3	31.90848544647262	33.0	31.0	34.0	30.0	34.0
4	35.69004378391712	37.0	35.0	37.0	33.0	37.0
5	35.4266311050814	37.0	35.0	37.0	33.0	37.0
6	35.48441354218056	37.0	35.0	37.0	33.0	37.0
7	35.451467686235816	37.0	35.0	37.0	33.0	37.0
8	35.4697983473113	37.0	35.0	37.0	33.0	37.0
9	37.04820855944746	39.0	37.0	39.0	33.0	39.0
10	36.876927109028124	39.0	37.0	39.0	33.0	39.0
11	37.08158608781451	39.0	37.0	39.0	33.0	39.0
12	37.00390046867292	39.0	37.0	39.0	33.0	39.0
13	37.075465589541196	39.0	37.0	39.0	33.0	39.0
14	38.04885606808091	40.0	37.0	41.0	33.0	41.0
15	38.04526393685249	40.0	37.0	41.0	33.0	41.0
16	37.839633695115936	39.0	37.0	41.0	33.0	41.0
17	37.99485076467686	39.0	37.0	40.0	33.0	41.0
18	38.127343364578195	40.0	38.0	41.0	34.0	41.0
19	38.18335286137148	40.0	38.0	41.0	34.0	41.0
20	38.214510360138135	40.0	38.0	41.0	34.0	41.0
21	38.199201406018744	40.0	38.0	41.0	34.0	41.0
22	38.12794462259497	40.0	37.0	41.0	34.0	41.0
23	38.04722187962506	40.0	37.0	41.0	34.0	41.0
24	37.94506968426246	40.0	37.0	41.0	33.0	41.0
25	37.84280957079428	39.0	37.0	41.0	33.0	41.0
26	37.62826837691169	39.0	37.0	40.0	33.0	41.0
27	37.44634928465713	39.0	36.0	40.0	33.0	41.0
28	37.30278737049827	39.0	36.0	40.0	32.0	41.0
29	37.075573507646766	39.0	35.0	40.0	32.0	41.0
30	36.9074216822891	39.0	35.0	40.0	32.0	41.0
31	36.59948507646769	38.0	35.0	40.0	31.0	41.0
32	36.368555747409964	38.0	35.0	40.0	31.0	41.0
33	36.06663172175629	38.0	35.0	40.0	30.0	41.0
34	35.94246423285644	38.0	35.0	40.0	30.0	41.0
35	35.784610878145045	38.0	35.0	40.0	30.0	41.0
36	35.65083867784904	38.0	35.0	40.0	29.0	41.0
37	35.48114516526887	38.0	35.0	40.0	28.0	41.0
38	35.26629563394179	38.0	35.0	40.0	27.0	41.0
39	34.8528305377405	38.0	34.0	40.0	25.0	41.0
40	34.63702516033547	37.0	34.0	40.0	24.0	41.0
41	34.34819005920079	37.0	33.0	40.0	23.0	41.0
42	34.03286877158362	37.0	33.0	40.0	21.0	41.0
43	33.07500308337445	36.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	1.0
16	0.0
17	3.0
18	3.0
19	6.0
20	11.0
21	43.0
22	40.0
23	100.0
24	150.0
25	247.0
26	379.0
27	585.0
28	806.0
29	1086.0
30	1478.0
31	1710.0
32	2274.0
33	2888.0
34	3851.0
35	5450.0
36	8478.0
37	13122.0
38	14810.0
39	7341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.321410952146024	18.469412925505672	16.64251356684756	21.56666255550074
2	19.39596694622595	22.68901085347805	33.18481746423286	24.730204736063147
3	20.797360631475087	24.631536753823386	33.352861371484956	21.218241243216575
4	14.17118894918599	18.8764183522447	31.055747409965466	35.89664528860384
5	13.35871978293044	37.46608288110508	31.80346571287617	17.37173162308831
6	29.330599407992107	33.05994079921066	17.476566354218058	20.13289343857918
7	24.472742969906268	34.16224716329551	20.2485199802664	21.11648988653182
8	31.24691662555501	30.750493339911202	18.85020966946226	19.152380365071533
9	27.119819930932415	12.452207696102615	19.67963739516527	40.74833497779971
10	20.459731129748395	27.599284657128763	26.49543660582141	25.44554760730143
11	32.216637888505176	23.27022693635915	18.157992106561423	26.35514306857425
12	20.293228909718795	31.724839664528858	26.219474592994573	21.76245683275777
13	33.77065860878145	18.711457819437594	25.787802170695613	21.730081401085346
14	23.108349777997038	23.248643315244202	27.36340651208683	26.27960039467193
15	29.099346324617663	25.271336951159352	23.57856438085841	22.05075234336458
16	22.49167488899852	25.02466699555994	28.15275037000493	24.330907745436605
17	19.309632461766157	31.171373951652686	26.49081154415392	23.028182042427233
18	20.38881351751357	23.180809077454366	30.244819930932415	26.185557474099653
19	22.23729649728663	27.230821410952146	31.657005426739023	18.8748766650222
20	22.84626295017267	22.556425752343365	32.82714602861371	21.77016526887025
21	26.672730636408488	22.232671435619142	30.357363098174645	20.73723482979773
22	24.460409472126297	24.572952639368527	29.1255550074001	21.84108288110508
23	22.295880611741488	25.75388505180069	29.902565367538237	22.047668968919584
24	21.546620621608287	26.194807597434632	31.14979033053774	21.10878145041934
25	21.147323630981745	26.43222742969906	29.78693882585101	22.63351011346818
26	22.57492599901332	25.10791810557474	30.836827824370992	21.480328071040947
27	21.427910705476073	25.804760730143066	30.01202516033547	22.75530340404539
28	20.2485199802664	25.730759743463246	32.51572520966946	21.504995066600888
29	21.438702516033548	25.166502220029603	32.060927479033055	21.3338677849038
30	20.142143561914157	26.48310310804144	32.88573014306858	20.489023186975828
31	20.011100148001972	27.027318697582636	31.715589541193882	21.24599161322151
32	20.129810064134187	27.064319190922546	32.61593487913172	20.189935865811545
33	19.309632461766157	27.46361618154909	33.02139861864825	20.20535273803651
34	20.038850518006907	27.653243709916133	30.974037987173165	21.3338677849038
35	19.258756783423777	26.788357178095705	32.93660582141095	21.01628021706956
36	19.463801184015786	27.19073754316724	30.377405032067095	22.968056240749878
37	18.449370991613222	26.84231623088308	31.56296250616675	23.14535027133695
38	18.981253083374444	24.690120868278242	32.64831031080415	23.680315737543168
39	19.355883078441046	24.401825357671434	34.02041193882585	22.221879625061668
40	18.541872224963	23.9994449925999	34.61858658115442	22.840096201282684
41	17.505858411445484	24.24148988653182	34.48754316724223	23.765108534780463
42	17.131228416378885	24.022570300937346	35.37092994573261	23.47527133695116
43	16.090589541193882	25.078626048347314	35.13659348791317	23.694190922545634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	6.5
2	12.0
3	67.5
4	123.0
5	123.0
6	180.0
7	237.0
8	267.0
9	297.0
10	434.0
11	571.0
12	571.0
13	1022.0
14	1473.0
15	2156.0
16	2839.0
17	2466.5
18	2094.0
19	2094.0
20	2178.0
21	2262.0
22	1598.5
23	935.0
24	833.0
25	731.0
26	731.0
27	785.5
28	840.0
29	882.0
30	924.0
31	995.5
32	1067.0
33	1067.0
34	1118.5
35	1170.0
36	1227.0
37	1284.0
38	1567.5
39	1851.0
40	1851.0
41	2203.5
42	2556.0
43	3056.0
44	3556.0
45	4280.0
46	5004.0
47	5004.0
48	5916.0
49	6828.0
50	6418.5
51	6009.0
52	5257.0
53	4505.0
54	4505.0
55	4367.5
56	4230.0
57	3880.5
58	3531.0
59	2992.0
60	2453.0
61	2453.0
62	2236.0
63	2019.0
64	1797.0
65	1575.0
66	1376.0
67	1177.0
68	1177.0
69	1072.0
70	967.0
71	845.0
72	723.0
73	587.0
74	451.0
75	451.0
76	373.5
77	296.0
78	223.5
79	151.0
80	111.0
81	71.0
82	71.0
83	53.0
84	35.0
85	23.0
86	11.0
87	7.0
88	3.0
89	3.0
90	2.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	64864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.42359398125308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.52466055471741	58.137025160335476
2	7.076708831379832	9.401208682782437
3	2.1097829871184866	4.20418105574741
4	1.083903910873854	2.8798717316230884
5	0.533828478588836	1.772940305870745
6	0.34350702100499014	1.3690182535767144
7	0.204247417894859	0.9496793290577208
8	0.17407450388766393	0.92501233349778
9	0.12997562956945571	0.7770103601381352
>10	0.7636068237205523	9.23316477553034
>50	0.025530927236857376	1.1500986679822398
>100	0.02088894046651967	3.22520966946226
>500	0.004641986770337704	2.112111494819931
>1k	0.004641986770337704	3.8634681795757277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1307	2.014985199802664	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1199	1.8484829797730635	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	816	1.2580167735569807	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	554	0.85409472126295	TruSeq Adapter, Index 8 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	463	0.713801184015787	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	367	0.5657992106561421	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.3715466206216083	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	223	0.3437962506166749	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	207	0.3191292550567341	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	183	0.2821287617168229	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.24358658115441542	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	134	0.20658608781450422	RNA PCR Primer, Index 8 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.17883571780957078	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	100	0.15416872224963	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84	0.1295017266896892	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72	0.1110014800197336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	69	0.1063764183522447	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66	0.1017513566847558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0015416872224963	0.0
7	0.0	0.0	0.0	0.0015416872224963	0.0
8	0.0	0.0	0.0	0.0015416872224963	0.0
9	0.0	0.0	0.0	0.0015416872224963	0.0
10	0.0	0.0	0.0	0.0015416872224963	0.0
11	0.0	0.0	0.0	0.0015416872224963	0.0
12	0.0	0.0	0.0	0.0015416872224963	0.0
13	0.0	0.0	0.0	0.0015416872224963	0.0
14	0.0	0.0	0.0	0.0015416872224963	0.0
15	0.0	0.0	0.0	0.0015416872224963	0.0
16	0.0	0.0	0.0	0.0030833744449926	0.0
17	0.0	0.0	0.0	0.0061667488899852	0.0
18	0.0	0.0	0.0	0.0061667488899852	0.0
19	0.0	0.0	0.0	0.0092501233349778	0.0
20	0.0	0.0	0.0	0.0107918105574741	0.0
21	0.0	0.0	0.0	0.0185002466699556	0.0
22	0.0	0.0	0.0	0.0231253083374445	0.0
23	0.0	0.0	0.0	0.0354588061174149	0.0
24	0.0	0.0	0.0	0.0585841144548594	0.0
25	0.0	0.0	0.0	0.0647508633448446	0.0
26	0.0	0.0	0.0	0.0801677355698076	0.0
27	0.0	0.0	0.0	0.1264183522446966	0.0
28	0.0	0.0	0.0	0.2250863344844598	0.0
29	0.0	0.0	0.0	0.416255550074001	0.0
30	0.0	0.0	0.0	0.7754686729156389	0.0
31	0.0	0.0	0.0	1.4615194869264923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTGGA	35	8.7790936E-4	26.428572	1
AAGCATG	40	0.0019117115	23.125002	24
GTACCTG	45	0.003787593	20.555557	35
GAAGCAT	45	0.003787593	20.555557	23
GGTATCA	690	0.0	20.376812	1
GTTCCGA	55	5.0715404E-4	20.181818	1
TAGGACC	50	0.0069652847	18.5	36
TATACAC	60	9.109356E-4	18.5	3
CTTAGGA	50	0.0069652847	18.5	34
CACGAAT	50	0.0069652847	18.5	12
CTTATTG	60	9.109356E-4	18.5	28
GGCCACA	50	0.0069652847	18.5	30
CCTTATT	60	9.109356E-4	18.5	27
CCTTAGG	50	0.0069652847	18.5	33
TGGCGTA	50	0.0069652847	18.5	18
ACGATGG	50	0.0069652847	18.5	25
ACGAATG	50	0.0069652847	18.5	13
GCCGTCT	75	2.029634E-4	17.266666	36
TTCCTCG	65	0.0015583102	17.076921	20
AATGGCG	65	0.0015583102	17.076921	16
>>END_MODULE