FastQCFastQC Report
Fri 10 Feb 2017
ERR1632364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT27911.892583120204604TruSeq Adapter, Index 6 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC1305.541346973572037No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT1134.816709292412617No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC893.7936913895993176RNA PCR Primer, Index 6 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC863.6658141517476555No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG532.2591645353793695No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG230.9803921568627451No Hit
ATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT130.5541346973572037RNA PCR Primer, Index 40 (95% over 21bp)
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT120.5115089514066496No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA100.4262574595055414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70.29838022165387895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2131287297527707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.2131287297527707No Hit
TCTCCGAGCCCACGAGACCGATCAGTACTCGTATGCCGTCTTC30.1278772378516624No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTA30.1278772378516624No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATG30.1278772378516624No Hit
CACCATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC30.1278772378516624No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC30.1278772378516624No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT30.1278772378516624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT501.2380588E-729.59999837
GCCGTCT501.2380588E-729.59999836
TGCCGTC651.2377768E-622.7692335
TATGCCG651.2377768E-622.7692333
TCTCGTA651.2377768E-622.7692328
ATGCCGT651.2377768E-622.7692334
TCTCCGA651.2377768E-622.769231
GTATGCC702.3646626E-621.14285732
ATCTCGT702.3646626E-621.14285727
CAGTATC702.3646626E-621.14285723
GTATCTC702.3646626E-621.14285725
AGTATCT702.3646626E-621.14285724
TCAGTAT702.3646626E-621.14285722
TATCTCG702.3646626E-621.14285726
CGTATGC702.3646626E-621.14285731
TCGTATG702.3646626E-621.14285730
CTCGTAT702.3646626E-621.14285729
AGCCCAC754.314201E-619.7333327
GCCCACG754.314201E-619.7333328
CCACGAG754.314201E-619.73333210
Produced by FastQC (version 0.11.5)